Modification of Chicken Egg Shell and Lime Extract (Citrus amblycarpa) for Minimizing Lead (Pb) Level on Blood Cockles (Anadara granosa)

Heavy metal in human body generally is from food consumed by the human either through fish or other aquatic animals in fresh water or seawater which are polluted by heavy metal. The presence of heavy metal in human body in certain level can cause several diseases, such as kidney disease, liver, nerve, and brain disease. Moreover, this research aimed at analyzing the decrease of lead (Pb) level on blood cockles (A. granosa) through engineering of stirring time, adsorption temperature, and variation of lime extract (C. amblycarpa) concentration by using Stirring Chamber tools through using chicken egg shell adsorbent. The research design used simple experiment in posttest only control group design. Meanwhile, the subject in this research was divided by two groups randomly in which a treatment was given to one group as control group and experiment group. Sample of blood cockles that was used was in 72 samples. Lime extract concentration in 1%, 1.5%, and 2% in stirring time of 5 minutes, 10 minutes, and 15 minutes before and after heating in 35°C utilized stirring speed in 150 rpm. Meanwhile, the chicken egg shell adsorbent was in 50 gram for each treatment. The sampling technique in this research utilized purposive sampling and data was analyzed by utilizing Statistic test of Two-Way Anova. Result showed that Pb level in fresh blood cockles before heating was 0.223 ppm and after heating, it reduced to be 0.062 ppm. Result of hypothesis test was obtained p-value <0.05, which was 0.000, and it indicated that there was a significant enhancement of chicken egg shell adsorption capacity in minimizing Pb level on blood cockles after being conducted the addition of lime extract with temperature variation and stirring time in certain stirring speed through engineering tool of Stirring Chamber. All in all, conclusion of this research was there was a significant enhancement of modified chicken egg shell adsorption capacity and lime extract in minimizing Pb level on blood cockles. Moreover, it was expected that this research could give contribution to food technology in reducing heavy metal of Pb level in pre-treatment of blood cockles (Anadara granosa) with modifying lime extract and chicken egg shell through Stirring chamber tool.