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Tooth for Truth-dental DNA for Gender Determination: a Review


Affiliations
1 Dept. of Pedodontics, A.B. Shetty Memorial Institute of Dental Sciences, Nitte University, Mangalore, India
2 Dept of Oral Medicine & Radiology, Mahe Institute of Dental Sciences, Chalakkara, Mahe UT of Puducherry, India
     

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Forensic odontology occupies a primary niche in the total spectrum of methods applied to medico legal identification that cannot be underrated. While extensive work has led to the rapid development of the subject of forensic odontology in Western countries, there is a need for heightening the awareness about the importance of this specialty amongst Indian dental professionals. The enamel and dentin of human teeth act like an armor coating to protect the DNA rich inner aspect of the tooth, that is, the pulp tissue from various environmental conditions.

Keywords

Forensic Odontology, DNA Analysis, Gender Determination
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  • Lund H & Mornstad H. Gender determination by odontometrics in Swedish population. J. of Forensic Odontostomatology 1999; 17(2):30-4.

  • I A Pretty& L D Addy: ‘‘Associated postmortem dental findings as an aid to personal identification.’’ Science& Justice 2002; 42(2).

  • Duffy JB, Waterfield JD, & Skinner MF: ‘‘Isolation of tooth pulp cells for sex chromatin studies in experimental dehydrated and cremated remains.’’ Forensic. Sci. Int 1991; 49(2): 127-41.

  • Jacqueline B Duffy: Isolation of tooth pulp cells from sex chromatin studies in experimental dehydrated and cremated remains. Forensic Sci.Int 49; 1991:127-141.

  • Barr ML, Bertam LF & Lindsay HA. The morphology of the nerve cell nucleus, according to sex, Anat. Rec. 1950; 107: 283.

  • Seno M & Ishizu H: ‘‘Sex identification of the human tooth.”Int .J. Forensic. Dent 1973; 1: 8-11.

  • Whittaker D K, Llewelyn D R & Jones R W: “Sex determination from necrotic pulp tissue.” BDJ 1975;139 (10): 403 – 5.

  • Johnson K Isaac, Bharath M Mody, Gopakumar R. A comparative study of three clinic-laboratory techniques in sex determination (unpublished data)

  • Saiki R.H: ‘‘Primer –directed enzymatic amplification of DNA with a thermo stable DNA polymerase. “Science 1988; 239: 487-491.

  • Sweet D,Hildebrand D, Philips D: ‘‘Identification of a skeleton using DNA from teeth and a PAP smear.’’ J. Forensic Sci 1999; 44(3):630-3.

  • Anupama Raina & TD Dogra: Application of DNA finger printing In medicolegal practice. Journal of Indian Medical Association 2002: 100(12).

  • Matt Ridley: Genome- The autobiography of a species in 23 chapters

  • Inman K Rudin N : An introduction to Forensic DNA Analysis. Boca Raton FL, CRC Press 1997

  • YamadaY, YamamotoK, YoshiiTetal:‘‘Analysis of DNA from tooth and application to forensic dental medicine.’’ Nippon Hoigaku Zasshi s1989; 43(5): 420-3. (PMID-2625734)

  • Sweet DJ, Sweet CH: ‘‘DNA analysis of dental pulp to link incinerated remains of homicide victim to crime scene.’’ J. Forensic. Sci 1995: 40(2): 310-14.

  • Sweet D & Hilderand DP: “Recovery of DNA from human teeth by cryogenic grinding.” J. Forensic. Sci 1998; 43: 1199.

  • I A Pretty & D Sweet: ‘‘A look at forensic dentistry- Part I: The role of teeth in the determination of human identity.’’ BDJ 2001: 190(7): 359-366.

  • Honda K, Harihara S, Nakamura T etal: ‘‘Sex identification by analysis of DNA extracted from hard tissues.’’Nippon Hoigaku Zasshi 1990; 44(4): 293-301. (PMID-2266607)

  • Dell’Osso G, Avitabile M, Sciacca G etal: ‘‘A polymorphism study of the DNA extracted from dental tissues.’’ Minerva Stomatology 1995; 44(5): 205-9. (PMID: 7476773)

  • Yamamoto K: ‘‘Molecular biological studies on teeth, and inquests.’’ Forensic. Sci. Int 1996: 80(1-2): 79-87.

  • Akane A, Hiroshi Shiono, Kazuo Matsubara, et al: “Sex identification of Forensic specimens by polymerase chain reaction (PCR); Two Alternative Methods”. Forensic. Sci. Int 1991; 49: 81-88.

  • Akane A, Shiono H, Matsubara K etal: ‘‘Purification of forensic specimens for the polymerase chain reaction (PCR) Analysis”. J .Forensic Sci 1993; 38 (3): 691-701.

  • Saiki R.H: ‘‘Primer –directed enzymatic amplification of DNA with a thermo stable DNA polymerase. “Science 1988; 239: 487-491.

  • Potsch L, Meyer U, Rothschild S etal : ‘‘Application of DNA techniques for identification using human dental pulp as a source of DNA.’’ Int-J-Legal-Med 1992; 105(3): 139-43.

  • Sivagami AV. Rao AU. Vaishney U. A sample and cost effective method for preparing DNA from the hard tooth tissue, and its use in PCR amplification of amelogenin gene segment for sex determination in Indian population. Forensic Sci Int. 2000; 110: 107-15.

  • Colson I B, Bailey J. F ,Vercauteren M etal: Ancient Biomol 1997, 1, 109-117

  • Poinar N H ,Hoss, Bada J L etal: Science 1996, 272,864-866

  • Hoss M, Jaruga P, Zastwany T H etal:Nucleic Acids Res 1996,24,1304-1307

  • Joachim Burger, Susanne Hummel, Bernd Hermann etal : Electrophoresis 1999, 20, 1722-1728

  • Chen L, Sun G, Wa M: ‘‘Influence exerted by environmental and physicochemical factors on the results of sex identification of human dental pulp by polymerase chain reaction.’’ Hua Xi Yi Ke Da Xue Xue Bao 1994; 25(3):253-8. (PMID 7896239)

  • Lessig R & Edelmann J: ‘‘Individualization of dental tissue — an aid for odontological identification?’’ J.Forensic Odontostomatoly 1995; 13(1): 1-3.

  • Urbani C, Lastrucci RD, Kramer B: ‘‘The effect of temperature on sex determination using DNAPCR analysis of dental pulp.’’ J.Forensic Odontostomatology 1999; 17(2):35-39.

  • Murakami H, Yamamoto Y, Yoshitome K, et al: ‘‘Forensic study of sex determination using PCR on tooth samples.’’ Acta Med-Okayama 2000; 54(1): 21-32. (PMID 10709619)

  • Komuro T, Nakamura M, Tsutsumi H etal: ‘‘Gender determination from dental pulp by using capillary gel electrophoresis of amelogenin locus.’’ J. Forensic Odontostomatology 1998; 16(2): 23-26.

  • Ovchinnikov IV, Octchinnikova OI, Druzina EB etal: ‘‘Molecular genetic sex determination of Medieval human remains from north Russia: comparison with archaeological and anthropological criteria.’’ Anthropol. Anz 1998; 56(1): 7-15. (PMID 9569974)

  • Malaver P.C, & Yunis J.J: ‘‘Different dental tissues as source of DNA for human identification in forensic cases.’’ Croat Med. J 2003; 44(3):306-9.

  • Hanaoka Y & Kiyoshi Minaguchi: “Sex determination from blood and teeth by PCR amplification of the Alphoid satellite family.”J. Forensic. Sci 1996; 41(5): 855-858

  • Stacks, B: “Sex determination of dried blood stains using the polymerase chain reaction with homologous X-Y-primers of the Zinc finger protein gene”. J. Forensic Sci 1996; 41(2): 287-90.

  • Lo Y, Mehal WZ, Fleming K. False positive results and the polymerase chain reaction Lacet 1990 1, 679

  • Persing DH .Polymerase chain reaction:Trenches to benches. J Clin Microbiol 1990, 29,1281-5

  • JG de Lomas, FJ Sunzeri, MP Busch. False negative results by polymerase chain reaction due to contamination by glove powder

  • Electrophoresis 1998 Jan;19(1):76-9.

  • Kashyap VK, Sahoo S, Sitalaximi T etal: “Deletions in theY-derived amelogenin gene fragment in the Indian population :BMC Med Genet 2006.7: 37.: Retrieved 2008-07-07.

  • Francès F, Portolés O, González JI etal: “Amelogenin test: From forensics to quality control in clinical and biochemical genomics.”.Clin Chim Acta. 2007, 386(1–2): 536.

  • Thangaraj K, Reddy AG, Singh L. “Is the amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis?”.Int J Legal Med 2002,116 (2): 121–3.


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  • Tooth for Truth-dental DNA for Gender Determination: a Review

Abstract Views: 1051  |  PDF Views: 2

Authors

Manju Gopakumar
Dept. of Pedodontics, A.B. Shetty Memorial Institute of Dental Sciences, Nitte University, Mangalore, India
R Gopakumar
Dept of Oral Medicine & Radiology, Mahe Institute of Dental Sciences, Chalakkara, Mahe UT of Puducherry, India

Abstract


Forensic odontology occupies a primary niche in the total spectrum of methods applied to medico legal identification that cannot be underrated. While extensive work has led to the rapid development of the subject of forensic odontology in Western countries, there is a need for heightening the awareness about the importance of this specialty amongst Indian dental professionals. The enamel and dentin of human teeth act like an armor coating to protect the DNA rich inner aspect of the tooth, that is, the pulp tissue from various environmental conditions.

Keywords


Forensic Odontology, DNA Analysis, Gender Determination

References