Inhibition of Antibiofilm Mediated Virulence Factors in Pseudomonas aeruginosa by Andrographis Paniculata

The focal intent of the study is about Quorum sensing (QS) which is well known for biofilm production, bacterial virulence and antibiotic resistance. Inhibition of quorum sensing in bacteria can reduce the risk of pathogenicity in all bacterial infection. In the present study the methanolic leaf extract of Andrographis paniculata has showed its anti quorum sensing activity against acyl homoserine lactone -dependent showed a concentration dependent (10-100 μg/ml) reduction in biofilm production in Pseudomonas aeruginosa and the inhibition level was upto 10-80%. Microtiter plate (MTP)assay and Light microscopic analysis has further confirmed the antibiofilm activity in Pseudomonas aeruginosa when treated with methanolic leaf extract of Andrographis paniculata. In addition the methanolic ectract of Andrographis paniculata itself showed no antibacterial activity in Pseudomonas aeruginosa.