Preparation and Evaluation of Pramipexole Dihydrochloride Loaded Chitosan Nanoparticles for Brain-Targeting

The present investigation was undertaken to develop nanoparticles of a hydrophilic drug pramipexole dihydrochloride and improve the entrapment efficiency of the drug. Pramipexole dihydrochloride nanoparticles where prepared with two methods by using Chitosan and another method by utilizing Chitosan, sodium alginate and Pluronic F-127. Five different trials were prepared with different concentrations of Chitosan in both methods. In two methods variables were found to have significant effect on the particle size, entrapment efficiency of drug which is influenced by Chitosan and sodium alginate. The maximum entrapment efficiency and least particle size were obtained with 3% Chitosan along with sodium alginate PS3 of 220.7 nm with 32.4 mV zeta potential with 91.2 % entrapment efficiency but in PC3 with 3% Chitosan show least particle size of 252nm with 44.7 mV zeta potential but less entrapment efficiency 82.8 % of than PS3.The surface morphology of PC3 and PS3 shows smooth surface with the in vitro release of PC3 with 90.2 %, PS3 with 96.12% in 24 hrs. PS3 shows better entrapment efficiency than PC3, Hence preparation methods along with two polymers may influence in entrapment efficiency of pramipexole dihydrochloride nanoparticles which meet the treatment of Parkinson's disease which has a potential influence on brain-targeting.