Isolation and Characterization of two New Flavonoids Derived from Leaves of Jatropha gossypifolia Linn

Jatropha gossypifolia L. (Euphorbiaceae), widely known as "bellyache bush," is a medicinal plant largely used throughout Africa and America for treatment of inflammatory analgesic antidiarrheal diseases. In spite of some published data on its efficiency, there are still few toxicological data unfolding the safety of this plant. The plant was extracted with many solvents (pet. ether, ethanol, and water). The preliminary phytochemical outcomes revealed that flavonoids and other glycosides as active constituents in ethanol extract of Jatropha gossypifolia. The ethanol extract of Jatropha gossypifolia was undertaken column chromatography with dissimilar solvent fractions. Two compounds were isolated from ethanol extract of Jatropha gossypifolia with the compound 1 was eluted with benzene: Chloroform 90:10 v/v and compound 2 were eluted with eluted with ethyl acetate: ethanol 80:20 v/v. The structures of the two isolated compounds were characterized by using FT-IR, NMR and Mass spectrophotometric methods. Thus, the compound 1 was characterized as 2-8 -4,5 -dihydroxy -6- (hydroxymethyl) - tetrahydro - 2H - pyran-2-yl) -2,5,6-trimethoxy -4-oxo-3,4 -dihydro-2H-chromen-7-yl) -6- (3,4-dihydroxyphenyl) -7- ethoxy -5-hydroxy-8-3-hydroxy-6-(hydroxymethyl) - 4,5 dimethoxy-tetrahydro-2Hpyran- 2-yl)-4H-chromen-4-one(C₄₃H₅₀O₂₀) and compound 2 was characterized as 2 -(2, 4-diethoxy-5- hydroxyphenyl)-7-(5-ethoxy-4-oxo-2-phenylchroman-7-yloxy)-5-hydroxy-8-6-(hydroxymethyl)-5-methoxytetrahydro- 2H-pyran-2-yl)-4H-chromen-4-one(C₄₃H₄₄O₁₃). Consequently, additional biological investigations essential to be carried out isolated compounds present in this plant.