Determination of Raltegravir by Derivative and Difference Spectrophotometric Techniques in Film Coated Tablets

Eight simple spectrophotometric techniques have been proposed for the determination of Raltegravir in pharmaceutical preparations. Three zero order (D₀), three first order derivative (D_!) and two difference spectrophotometric methods have been developed in for the determination of Raltegravir in borate buffer (pH 9), hydrochloric acid and sodium acetate buffer solutions. Linearity has been followed over the concentration range 1-150, 10-150 and 10-150 μg/mL in all the buffers in zero order, first order derivative and difference spectroscopy respectively. All the methods were validated and can be very successfully applied for the determination of Raltegravir in pharmaceutical formulations.