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Effect of Host Trees on Berberine Content in Stem of Tinospora cordifolia Climber:A Preliminary Study


Affiliations
1 Lovely Professional University, Phagwara, Punjab, India
2 Herbal Health Research Consortium, Amritsar, Punjab, India
     

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Present study was aimed to analyze berberine concentration in Tinospora cordifolia stems collected from Ficus, Azadirachta, Syzygium, Populus and Eucalyptus. High Performance Liquid Chromatography equipped with UV detector was used for analytical study of berberine from the stem of Tinospora cordifolia. Methanol (100%) as a diluent was used for sample extraction. Using potassium dihydrogen phosphate and acetonitrile as a mobile phase in a ratio of 65 to 35 respectively and Zorbax XDB C-18, 250x 4.6mm 5-micron column, berberine was separated at 254nm wavelength. Maximum concentration of berberine was detected in the stem of Tinospora cordifolia climbed on Ficus (0.087%) followed by Azadirachta (0.084%). The least concentration of berberine was quantified in Eucalyptus (0.011%) on dried wt. basis.

Keywords

Berberine, Tinospora cordifolia, HPLC Chromatograms.
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  • Effect of Host Trees on Berberine Content in Stem of Tinospora cordifolia Climber:A Preliminary Study

Abstract Views: 157  |  PDF Views: 1

Authors

Wazbir Hussain Barlaskar
Lovely Professional University, Phagwara, Punjab, India
Neeta Raj Sharma
Lovely Professional University, Phagwara, Punjab, India
Gaurav Kumar
Lovely Professional University, Phagwara, Punjab, India
Sanjeev Kataria
Herbal Health Research Consortium, Amritsar, Punjab, India

Abstract


Present study was aimed to analyze berberine concentration in Tinospora cordifolia stems collected from Ficus, Azadirachta, Syzygium, Populus and Eucalyptus. High Performance Liquid Chromatography equipped with UV detector was used for analytical study of berberine from the stem of Tinospora cordifolia. Methanol (100%) as a diluent was used for sample extraction. Using potassium dihydrogen phosphate and acetonitrile as a mobile phase in a ratio of 65 to 35 respectively and Zorbax XDB C-18, 250x 4.6mm 5-micron column, berberine was separated at 254nm wavelength. Maximum concentration of berberine was detected in the stem of Tinospora cordifolia climbed on Ficus (0.087%) followed by Azadirachta (0.084%). The least concentration of berberine was quantified in Eucalyptus (0.011%) on dried wt. basis.

Keywords


Berberine, Tinospora cordifolia, HPLC Chromatograms.