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Derivative Spectrophotometric Estimation of Amoxicillin Trihydrate and Carbocisteine by Third Order Derivative Spectroscopy Method in Combined Dosage Form


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1 Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai-400016, India
     

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In the proposed method UV spectrophotometric, third order derivative method was developed for validation of amoxicillin tri-hydrate and carbocisteine from pharmaceutical formulation. The ICH guidelines were used for validation of amoxicillin and carbocisteine. The derivative method involves the measurement of absorbance, hence wavelengths 236.2 nm and 210 nm were found to be suitable for the estimation of amoxicillin and carbocisteine respectively. The linearity was found in the concentration range of 10 to 100 μg/ml for amoxicillin and 1 to 10 μg/ml for carbocisteine respectively. For amoxicillin the percentage mean recovery was found to be 100.075% and for carbocisteine was 100.031% respectively. The linearity, accuracy and precision of method was validated by statistically. The less percentage (%) RSD values indicates both intra and inter day have high grade of precision.

Keywords

Third Order Derivative UV Spectrophotometric Estimation, Amoxicillin Trihydrate, Carbocisteine.
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  • Derivative Spectrophotometric Estimation of Amoxicillin Trihydrate and Carbocisteine by Third Order Derivative Spectroscopy Method in Combined Dosage Form

Abstract Views: 201  |  PDF Views: 1

Authors

Rajan V. Rele
Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai-400016, India

Abstract


In the proposed method UV spectrophotometric, third order derivative method was developed for validation of amoxicillin tri-hydrate and carbocisteine from pharmaceutical formulation. The ICH guidelines were used for validation of amoxicillin and carbocisteine. The derivative method involves the measurement of absorbance, hence wavelengths 236.2 nm and 210 nm were found to be suitable for the estimation of amoxicillin and carbocisteine respectively. The linearity was found in the concentration range of 10 to 100 μg/ml for amoxicillin and 1 to 10 μg/ml for carbocisteine respectively. For amoxicillin the percentage mean recovery was found to be 100.075% and for carbocisteine was 100.031% respectively. The linearity, accuracy and precision of method was validated by statistically. The less percentage (%) RSD values indicates both intra and inter day have high grade of precision.

Keywords


Third Order Derivative UV Spectrophotometric Estimation, Amoxicillin Trihydrate, Carbocisteine.