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Isolation and Screening the Pharmacological Activities of Vegetative and Spore-Crystal Proteins from Bacillus thuringiensis
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Bacillus thuringiensis, the most successful and most widely used microbial insecticide, produces crystal proteins. The physiological significance of the crystal proteins poorly understood except for the potent insecticidal activity. In the current study, an attempt made to isolate vegetative and spore-crystal proteins from Bacillus thuringiensis NCIM2514 and to perform their hemolytic and antioxidant activities. The proteins were separated by salting out methods then by using SDS-PAGE. The molecular protein weight of the vegetative and spore-crystal protein identified as the presence of low molecular weight protein. Pharmacological activities as antioxidant and hemolytic studies were performed for the crude proteins and found as Spore-crystal proteins contain more anti-oxidant when compared to vegetative proteins.
Keywords
Bacillus thuringiensis (Bt), Spore-Crystal Proteins, Vegetative Proteins. SDS-PAGE, Antioxidant.
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