Fabrication and In-Vitro Evaluation of Liposomal Quercetin and its Optimization

This study intended to explore the influence of formulation factors on the physico-chemical properties of quercetin-loaded liposomes and optimize the fabrication environment. Thin film hydration technique was employed to prepare liposome and optimization was done by 32 factorial designs combined with desirability function. Nine preparations were prepared by using altered drug: lipid and soyphosphatidylcholine: cholesterol (SPC: cholesterol) ratios and assessed for entrapment efficacy and vesicle size. The findings were the mean diameter and drug encapsulation efficiency. Results exhibited that SPC concentration and SPC: cholesterol molar ratio had a solid impact on liposome size. Increasing the lipid ratio produced a reduction in size. The degree of quercetin charging depended on the factors evaluated. Increasing SPC concentration and lipid ratio pointed lyboosted quercetin entrapment. However, higher quercetin concentrations had a negative effect on drug entrapment. Based on this, an optimized design was determined, prepared and investigated. The entrapment efficiency and vesicle size were found to be very adjacent with the predicted values. The formulation was found to be globular shape and also shows sustained release pattern. These results backing the fact that 32 full factorial designs with desirability function might be efficiently used in optimization of quercetin loaded liposome. The overall results showed that SPC concentration and lipid ratio were the key features influencing particle size, while entrapment efficiency was affected primarily by quercetin concentration.