Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Green Synthesis of Antibacterial Silver Nanoparticles using Yeast Isolates and its Characterization


Affiliations
1 Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India
     

   Subscribe/Renew Journal


The present study had investigated the role of yeasts, isolated from soil and food samples for its ability to synthesize silver nanoparticles. Serial dilution method followed by spread plate technique was used for isolation of yeasts on YEPD agar medium and the morphological characterization was done by simple staining technique. Primary screening was done by visual colour change followed by UV spectrophotometric analysis. Significant color change and prominent peaks around 420-450 nm were noted through UV spectrophotometric analysis in the cell free extract of Isolate 5, Isolate 8 and Isolate 13, revealed the synthesis of Ag-NPs. Optimization of growth parameters for the enhancement of Ag-NPs production was evaluated for each of the potent yeast isolates. Isolate 5 and 13 had showed highest Ag-NPs production after 24 h of incubation period, supplemented by fructose in the production medium of pH 9 along with 5mM substrate concentration of AgNO3, at 45˚C, whereas in case of isolate 8, highest production was observed with 3mM concentration of Ag NO3 at pH 8. FTIR analysis revealed the presence of significant peaks around 3441-3255, 2922-2964, 1576-1579, 1384-1415, 1276, 1153 and 1076cm-1 of Ag-NPs synthesized by isolate 5,8 and 13 respectively, were corresponding to the functional groups produced during the synthesis of Ag-NPs. XRD results showed peaks at 2 theta values 27.29°, 31.76°, 45.91°, 54.26-56.77° and 75.56-75.99°, were corresponding to (111), (200), (222), (311), and (331) lattice of silver nanoparticles. Distinct surface topology of the AgNPs synthesised by the potent yeast isolates were observed by AFM analysis. Positive anti-oxidant and anti-bacterial activity were elucidated by AgNPs synthesised by the potent yeast isolates, wherein AgNPs were found to be bacteriostatic at low concentration (5ug/ml) and bactericidal at high concentrations (100ug/ml).

Keywords

Yeast Strains, Silver Nanoparticles, Optimization, FTIR, XRD, AFM, Antibacterial Activity.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Eugenio, Mateus, et al. "Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria." RSC Advances 6.12 (2016): 9893-9904.

  • BoroumandMoghaddam, Amin, et al. "Nanoparticles biosynthesized by fungi and yeast: a review of their preparation, properties, and medical applications." Molecules 20.9 (2015): 16540-16565.

  • Gajbhiye, Monali, et al. "Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole." Nanomedicine: Nanotechnology, Biology and Medicine 5.4 (2009): 382-386.

  • Nayak, RatiRanjan, et al. "Green synthesis of silver nanoparticle by Penicilliumpurpurogenum NPMF: the process and optimization." Journal of Nanoparticle Research 13.8 (2011): 3129-3137.

  • Kowshik, Meenal, et al. "Extracellular synthesis of silver nanoparticles by a silver-tolerant yeast strain MKY3." Nanotechnology 14.1 (2002): 95.

  • Shahverdi, Ahmad R., et al. "Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli." Nanomedicine: Nanotechnology, Biology and Medicine 3.2 (2007): 168-171.

  • Durán, Nelson, et al. "Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains." Journal of nanobiotechnology 3.1 (2005): 8.

  • Ghodake, Gajanan, Yeong Deuk Seo, and Dae Sung Lee. "Hazardous phytotoxic nature of cobalt and zinc oxide nanoparticles assessed using Allium cepa." Journal of hazardous materials 186.1 (2011): 952-955.

  • Ahmad, Absar, et al. "Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum." Colloids and surfaces B: Biointerfaces 28.4 (2003): 313-318.

  • Jha, Anal K., and K. Prasad. "PbS nanoparticles: biosynthesis and characterization." International Journal of Nanoparticles 5.4 (2012): 369-379.

  • Saxena, J., Sharma, P. K., Sharma, M. M., and Singh, A. Process optimization for green synthesis of silver nanoparticles by Sclerotinia sclerotiorum. Springer Plus5.1 (2016): 1-10.

  • Maiti, Swarnali, et al. "Antimicrobial activities of silver nanoparticles synthesized from Lycopersicon esculentum extract." Journal of Analytical Science and Technology 5.1 (2014): 40.

  • Nagaich, U., Gulati, N., and Chauhan, S. "Antioxidant and Antibacterial Potential of Silver Nanoparticles: Biogenic Synthesis Utilizing Apple Extract." Journal of Pharmaceutics, (2016).

  • Shaligram, Nikhil S., et al. "Biosynthesis of silver nanoparticles using aqueous extract from the compactin producing fungal strain." Process Biochemistry 44.8 (2009): 939-943.

  • Alahmad, Abdalrahim, et al. "Preparation of colloidal silver nanoparticles and structural characterization." Physical Sciences Research International 1.4 (2013): 89-96.

  • Geetha, A.R., et al. "Optimization of green synthesis of silver nanoparticles from leaf extracts of Pimenta dioica (Allspice)." The Scientific World Journal (2013).

  • Madhiyazhagan, Pari, et al. "Sargassum muticum-synthesized silver nanoparticles: an effective control tool against mosquito vectors and bacterial pathogens." Parasitology research 114.11 (2015): 4305-4317.

  • Thakkar, K. N., Mhatre, S. S., and Parikh, R. Y. Biological synthesis of metallic nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine 6.2 (2010): 257-262.

  • Hemath Naveen, K.S., et al. “Extracellular biosynthesis of silver nanoparticles using the filamentous fungus Penicillium sp.”Arch. Appl. Sci. Res 2.6 (2010):161-167.


Abstract Views: 273

PDF Views: 0




  • Green Synthesis of Antibacterial Silver Nanoparticles using Yeast Isolates and its Characterization

Abstract Views: 273  |  PDF Views: 0

Authors

Justina Daphne
Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India
Asha Francis
Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India
Ria Mohanty
Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India
Nupur Ojha
Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India
Nilanjana Das
Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India

Abstract


The present study had investigated the role of yeasts, isolated from soil and food samples for its ability to synthesize silver nanoparticles. Serial dilution method followed by spread plate technique was used for isolation of yeasts on YEPD agar medium and the morphological characterization was done by simple staining technique. Primary screening was done by visual colour change followed by UV spectrophotometric analysis. Significant color change and prominent peaks around 420-450 nm were noted through UV spectrophotometric analysis in the cell free extract of Isolate 5, Isolate 8 and Isolate 13, revealed the synthesis of Ag-NPs. Optimization of growth parameters for the enhancement of Ag-NPs production was evaluated for each of the potent yeast isolates. Isolate 5 and 13 had showed highest Ag-NPs production after 24 h of incubation period, supplemented by fructose in the production medium of pH 9 along with 5mM substrate concentration of AgNO3, at 45˚C, whereas in case of isolate 8, highest production was observed with 3mM concentration of Ag NO3 at pH 8. FTIR analysis revealed the presence of significant peaks around 3441-3255, 2922-2964, 1576-1579, 1384-1415, 1276, 1153 and 1076cm-1 of Ag-NPs synthesized by isolate 5,8 and 13 respectively, were corresponding to the functional groups produced during the synthesis of Ag-NPs. XRD results showed peaks at 2 theta values 27.29°, 31.76°, 45.91°, 54.26-56.77° and 75.56-75.99°, were corresponding to (111), (200), (222), (311), and (331) lattice of silver nanoparticles. Distinct surface topology of the AgNPs synthesised by the potent yeast isolates were observed by AFM analysis. Positive anti-oxidant and anti-bacterial activity were elucidated by AgNPs synthesised by the potent yeast isolates, wherein AgNPs were found to be bacteriostatic at low concentration (5ug/ml) and bactericidal at high concentrations (100ug/ml).

Keywords


Yeast Strains, Silver Nanoparticles, Optimization, FTIR, XRD, AFM, Antibacterial Activity.

References