Phytochemical and Physiochemical Standardization of a Siddha Formulation Seenthil Chooranam

The aim of the present study is to investigate the phytochemical and physiochemical investigation of a traditional classical Siddha formulation known as Seenthil chooranam (SC). Preliminary phytochemical analysis, HPTLC analysis and physicochemical parameters such as ash values, extractive values and loss on drying were determined as per standard protocols. The SC upon successive extraction with petroleum ether, chloroform, ethyl acetate and ethanol gave a yield of 0.05, 0.33, 1.50 and 1.90%w/w respectively. Phytochemical analysis of different extracts gave positive test for alkaloids, steroids, terpenoids, flavonoids, tannins, carbohydrates, glycosides, and proteins. HPTLC finger print analysis of the extracts showed the presence of possible number of components. Physicochemical parameters such as total ash, water soluble ash and acid insoluble ash values were found to be 2.98, 1.62 and 1.04%w/w, respectively; extractive value were found to be alcohol soluble 10.20 %w/w, water soluble- 10.24%w/w and moisture content– 0.105% w/w. The present study provides phytochemical and physicochemical details of SC which are useful in laying down standardization and pharmacopoeia parameters.