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Detection of Legionella Spp. in Public Hospitals Water Supplies in Lattakia-Syria


Affiliations
1 Department of Microbiology, Tishreen University, Lattakia, Syrian Arab Republic
2 Department of Medical Laboratory, Tishreen University, Lattakia, Syrian Arab Republic
     

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The present study was designed to evaluate the occurrence of Legionella spp. contamination in water taps and water tanks of public hospitals, Lattakia, Syria. It is the first study about Legionella in Syria. One hundred and thirty swabs from water taps and 26L of water from water tanks of seven hospitals were collected and detected for the presence of Legionella spp. by using GVPC, BCYE, BCY agar. The results were 49 of 130 (37.69%) water tap samples which were positive for Legionella spp, and most water tank samples were positive for Legionella spp.,too . In conclusion, Legionella spp. are present in aquatic hospitals environment of Lattakia, Syria, so hospital water systems may be the primary reservoirs for Legionnaires’ disease. Due to the serious risk of infections, it is better to make efforts to eliminate Legionella spp. in water supplies in Hospitals.

Keywords

Legionella spp., Legionelosis, Water System, GVPC, BCY and BCYE.
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  • Cloud JL, Carroll KC, Pixton P, Erali M, Hillyard DR. Detection of legionella species in respiratory specimens using PCR with sequencing confirmation. J Clin Microbiol 2000;38(5):1709-12.
  • Jonas D, Rosenbaum A, Weyrich S, Bhakdi S. Enzyme-linked immunoassay for detection of PCR-amplified DNA of legionellae in bronchoal veolar fluid. J Clin Microbiol 1995;33(5):1247-52.
  • Zhan XY, Li LQ, Hu CH, Zhu QY. Two-step scheme for rapid identification and differentiation of Legionella pneumophila and non-Legionella pneumophila species. J Clin Microbiol 2010;48(2):433-9.
  • Wellinghausen N, Frost C, Marre R. Detection of Legionellae in hospital water samples by quantitative real-time Light Cycler PCR. Appl Environ Microbiol 2001;67(9):3985-93.
  • Reischl U, Linde HJ, Lehn N, Landt O, Barratt K, Wellinghausen N. Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-colorreal-time PCR and melting curve analysis. J Clin Microbiol 2002;40(10):3814-7.
  • Squinazi F., Nahapetian K. - Legionella pneumophila methods de recherche chezl’ homme et dansl’ environnement - Revue française des laboratoires, avril 1988, Vol.172, p 33-41.
  • www.biomerieux-diagnositics.com
  • Centers for Disease Control and Prevention (CDC) (2005b). "Procedures for the Recovery of Legionella from the Environment."

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  • Detection of Legionella Spp. in Public Hospitals Water Supplies in Lattakia-Syria

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Authors

Eiman Shahrour
Department of Microbiology, Tishreen University, Lattakia, Syrian Arab Republic
Atiia Khamees
Department of Microbiology, Tishreen University, Lattakia, Syrian Arab Republic
Iyad Almahmoud
Department of Medical Laboratory, Tishreen University, Lattakia, Syrian Arab Republic

Abstract


The present study was designed to evaluate the occurrence of Legionella spp. contamination in water taps and water tanks of public hospitals, Lattakia, Syria. It is the first study about Legionella in Syria. One hundred and thirty swabs from water taps and 26L of water from water tanks of seven hospitals were collected and detected for the presence of Legionella spp. by using GVPC, BCYE, BCY agar. The results were 49 of 130 (37.69%) water tap samples which were positive for Legionella spp, and most water tank samples were positive for Legionella spp.,too . In conclusion, Legionella spp. are present in aquatic hospitals environment of Lattakia, Syria, so hospital water systems may be the primary reservoirs for Legionnaires’ disease. Due to the serious risk of infections, it is better to make efforts to eliminate Legionella spp. in water supplies in Hospitals.

Keywords


Legionella spp., Legionelosis, Water System, GVPC, BCY and BCYE.

References