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Light Green Stain, Nuclear Fast Red Stain used an Alternative Routine Haematoxylin and Eosin Stain


Affiliations
1 Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, India
     

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Objective:-1)To compare the intensity of light green stain as cytoplasm and nuclear fast red as nuclear stain compare with routine haematoxylin and eosin stain.2)To evaluate the accuracy of light green stain and nuclear fast red as an alternative stain used in cytology. Materials and methods: 30 buccal smears were collected from 10 healthy patients. they were categorized into 3 groups according to type stains were used. Result: this present study showed statically significant, p value >0.001.Conclusion:Several modifications have been made to improve their efficiency, There has been a rising need for efficient, accurate and less complex staining procedures is required

Keywords

Stains , Modified Stains.
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  • Anderson J. (2011). An introduction to Routine and special staining. Retrieved on August 18, 2014 from. Godwin, A. (2011).
  • Histochemical uses of haematoxylin-a review. Retrieved August 18, 2014 From www.arpapress.comHarris, T. J., & McCormick, F. (2010).
  • The molecular pathology of cancer. Nat Rev Clin Oncol, 7(5), 251-256.

Abstract Views: 254

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  • Light Green Stain, Nuclear Fast Red Stain used an Alternative Routine Haematoxylin and Eosin Stain

Abstract Views: 254  |  PDF Views: 0

Authors

Aiman Zafar
Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, India
Pratibha Ramani
Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, India
Herald J. Sherlin
Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, India
Gheena
Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, India
R. Abhilasha
Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, India
Giffrina Jayaraj
Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, India
K. R. Don
Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, India
S. Archana
Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, India

Abstract


Objective:-1)To compare the intensity of light green stain as cytoplasm and nuclear fast red as nuclear stain compare with routine haematoxylin and eosin stain.2)To evaluate the accuracy of light green stain and nuclear fast red as an alternative stain used in cytology. Materials and methods: 30 buccal smears were collected from 10 healthy patients. they were categorized into 3 groups according to type stains were used. Result: this present study showed statically significant, p value >0.001.Conclusion:Several modifications have been made to improve their efficiency, There has been a rising need for efficient, accurate and less complex staining procedures is required

Keywords


Stains , Modified Stains.

References