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Effects of Zinc Oxide Nanoparticles on the Growth Inhibition of Fungi Isolated From Cream Cosmetics


Affiliations
1 Department of Biology, University of Kufa, 21-Najaf, Iraq
     

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In this study fungi were isolated from cosmetic industries, 10cosmetics samples which applied to facial skin were tested for the presence of contaminating with fungi. Three species of fungi were isolated from the cosmetics namely Aspergillus flavus, A. niger and Candida albicans. The sabouraud dextrose agar was used to isolate fungal contaminated cosmetics. These fungi were opportunistic pathogens and may cause skin irritation, infections especially in wounded epithelium and immunocompromised consumers. Candida albicans grow on chrom agar after 2days. A. flavus is producing aflatoxin BI, which proved by high performance liquid chromatography (HPLC). Zinc oxide nanoparticles (ZnO NPs) using an alternative to fungicides controlling development is carried out and investigated. ZnO NPs proved high efficiency to decrease growth of Aspergillus flavus, A. niger and Candida albicans by percentage of (80, 85, 90) % respectively in the concentration of 50 ppm; while 150 ppm of ZnO causes complete inhibition growth of fungi under study as compared with a control group which untreated with ZnO NPs. SEM show changing in Aspergillus flavus and A.niger when treated with ZnO NPs which decreased in the number of fungi cells, mycelium and conidia,as well as the opening of conidia as compared with the control group which untreated with ZnO NPs.

Keywords

Cosmetics, Opportunistic Fungi, Zinc Oxide Nanoparticles, HPLC, SEM.
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  • Dubchak S, Ogar A , Mietelski J W, Turnau K. Influence of silver and tianium nanoparticles on arbuscular mycorhiza colonization and accumulation of radiocaesium in Helianthus anus. Spanish journal of Psychology. 2010; 8(1): 103-108.

  • Birajdar G O, Kadam V S, Chintale A G, Halle P D, Nabde M K, Maske K S. A Comprehensive review on nanotechnology. Research J. Pharm. and Tech. 2013; 6(5): 486-495.

  • Szabó T, Németh J, Dékány I. Zinc oxide nanoparticles incorporated in ultra thin layer silicate films and their photocatalytic properties.Colloids Surfaces, Physicochemical and Engineering Aspects journal. 2003; 2(30): 23-25.

  • Pavani K.V, Sunil Kumar N, Sangameshwaran B B. Synthesis of lead nanoparticles by Aspergillus species. Polish Journal of Microbiology. 2012; 61 (1): 61–63.

  • Ananpattarachai J, Yuphada B, Puangrat K. Visible light photocatalytic antibacterial activity of Ni- doped and N-doped TiO2 on Staphylococcus aureus and Escherichia coli bacteria. Environmental Science and Pollution Research journal. 2016;23(5): 4111-4119.

  • Brayner R , Dahoumane S A, ClaudeYéprémian C, Djediat C, Meyer M, Alain Couté A.,Fiévet F. ZnO nanoparticles: synthesis, characterization, and ecotoxicological studies. Langmuir Journal. 2010; 26: 6522-6528.

  • Karimiyan A, Najafzadeh H , Ghorbanpour M, Hekmati Moghaddam S H. Antifungal effect of magnesium oxide, zinc oxide, silicon oxide and copper oxide nanoparticles against candida albicans. Zahedan Journal of Research in Medical Sciences. 2015;17 (10): 2179.

  • Muhammed H J. Bacterial and fungal contamination in three brands of,cosmetic marketed in Iraq. Iraq Journal of Pharmaceutical Sciences. 2011; 20: 38-42.

  • Baskar G, Chandhuru J, Sheraz F K, Praveen A S. Mycological synthesis, characterization and antifungal activity of zinc oxide nanoparticles. Asian J. Pharm. Tech. 2013; 3(4): 142-146.

  • Osungunna M O, Oluremi B B, Adetuyi A. Bacteriological and antibiotic sensitivity patterns of bacterial isolates from creams and lotions hawked in Sagamu Ogun State. Pakistan Journal of Nutrition. 2010; (9): 773.

  • Yorgancioglu A, Bayramoglu E E. Production of cosmetics purpose collagen containing antimicrobial emulsion with certain essentials oils. Industrial Crops and Products is an International Journal. 2013;44: 378-382.

  • Lundov M D, Moesby L, Zachariae C, Johansen J D. Contamination versus preservation of cosmetics: a review on legislation, usage, infections, and contact allergy. American Journal of Contact Dermatitis. 2009; 60: 70-78.

  • Tamali M, Gamal M A, Alghazal M A. Microbiological quality assessment of some brands of cosmetic eye preparations sold in Libyan markets. International Journal of Science and Research.2013;4: 1349-1355.

  • Chen Y C, Cheng H Y, Yang C F, Hsieh Y T. Investigation of the optimal parameters in hydrothermal methods for the synthesis of ZnO nanorods. Journal of Nanomaterials. 2014;vol. : pp245

  • Dashen M M, Patricia F C, Juliet N O, Josephine A. M. Microbiological quality assessment of some brands of, cosmetics powders sold within Jos Metropolis, Plateau State. Journal of Microbiology and Biotechnology Research. 2011;1 (2): 101-106.

  • Behravan J, Bazzaz F, Malaekeh P. Survey of bacteriological contamination of cosmetic creams in Iran .International Journal of Dermatology. 2005; 21(44): 482-485.

  • Pinon A, Alexandre V, Cupferman S, Crozier A, vialette M. Growth, survival and inactivation of Pseudomonas aeruginosa and Staphylococcus aureus strains of various origin in the presence of ethanol. International Journal of Cosmetic Science. 2007; 29: 111-119.

  • Afifi N A, Ramadan A, El-Kashoury E A, El-Banna H A. Some pharmacological activities of essential oils of certain umbelliferous fruits. Veterinary medical journal Giza. 1994; 42(3) : 85.

  • Nabawy G A, Hassan A A, Sayed El-Ahl R H, Refai M K. Effects of metal nanoparticles in comparison with commercial antifungal feed additives on the growth of Aspergillus flavus and alfatoxin b1 production. Journal of Global Biosciences. 2014; 6(3): 54-71.

  • Hiral S, Nirmal K, Khushal P, Rita N. Metallic nanoparticles as alternative antimicrobials. Journal of Nanomedicine Research. 2017; 6 (4).

  • Azam A, Arham S Ahmed, M O, Mohammad S, Khan S.S. Antimicrobial activity of metal oxide nanoparticles against GramPositive and Gram-Negative Bacteria: A comparative study. International Journal of Nanomedicine. 2012; 1: 6003-6009.

  • Savi G D, Bortoluzzi A J, Scussel V M. Antifungal properties of zinc-compounds against toxigenic fungi and mycotoxin. International Journal of Food Science and Technology. 2013; 48: 1834–1840.

  • Lili H, Yang L, Azlin M, Mengshi L. Antifungal activity of zinc oxide nanoparticles against Botrytis cinerea and Penicillium expansum. Microbiological Research Journal. 2011;166(3): 207– 215.


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  • Effects of Zinc Oxide Nanoparticles on the Growth Inhibition of Fungi Isolated From Cream Cosmetics

Abstract Views: 201  |  PDF Views: 0

Authors

R. A. Sahib
Department of Biology, University of Kufa, 21-Najaf, Iraq

Abstract


In this study fungi were isolated from cosmetic industries, 10cosmetics samples which applied to facial skin were tested for the presence of contaminating with fungi. Three species of fungi were isolated from the cosmetics namely Aspergillus flavus, A. niger and Candida albicans. The sabouraud dextrose agar was used to isolate fungal contaminated cosmetics. These fungi were opportunistic pathogens and may cause skin irritation, infections especially in wounded epithelium and immunocompromised consumers. Candida albicans grow on chrom agar after 2days. A. flavus is producing aflatoxin BI, which proved by high performance liquid chromatography (HPLC). Zinc oxide nanoparticles (ZnO NPs) using an alternative to fungicides controlling development is carried out and investigated. ZnO NPs proved high efficiency to decrease growth of Aspergillus flavus, A. niger and Candida albicans by percentage of (80, 85, 90) % respectively in the concentration of 50 ppm; while 150 ppm of ZnO causes complete inhibition growth of fungi under study as compared with a control group which untreated with ZnO NPs. SEM show changing in Aspergillus flavus and A.niger when treated with ZnO NPs which decreased in the number of fungi cells, mycelium and conidia,as well as the opening of conidia as compared with the control group which untreated with ZnO NPs.

Keywords


Cosmetics, Opportunistic Fungi, Zinc Oxide Nanoparticles, HPLC, SEM.

References