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Hypoglycemic Activity of Ethyl Acetate Fraction of Mengkudu Fruit (Morinda citrifolia L.) in Mice Induced Alloxan


Affiliations
1 Department of Pharmacology and Pharmacy Clinic, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, Indonesia
2 Department of Pharmaceutical Biology, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, Indonesia
     

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Complementary medicine for diabetes mellitus using medicinal plants is the solution chosen as an alternative treatment. Mengkudu fruit (Morinda citrifolia L.) is empirically used by the community for the treatment of diabetes mellitus. To study the hypoglycemic activity of subfraction mengkudu fruit in mice induced alloxan. The test results on subfraction of ethyl acetate fraction (dose 150 mg/kgBW) induced alloxan (dose 175 mg/kgBW) showed that there was a decrease in blood glucose levels including Mc-IIb (33.94%), Mc-IIc (32.21%), Mc-IIa (29.58%), Mc-IId (18.16%) and Mc-IIe (8.09%) were significantly different compared to the negative control (p<0.05). Subfraction Mc-IIb shows that the most active hypoglycemic activity may be due to some secondary metabolites such as flavonoids, saponins, alkaloids, and polyphenols as antidiabetic bioactive principles. These research showed that Mc-IIb subfraction indicates the highest hypoglycemic activity. However, the research for the active hypoglycemic compound of Mc-IIb subfraction needs to be explored further.

Keywords

Morinda citrifolia, Ethyl Acetate Fraction, Subfraction, Hypoglycemic, Alloxan.
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  • International Diabetes Federation. Diabetes Atlas 8th edition. Diabetes Atlas. 2017

  • Novo Nordisk. Where Economics and Health Meet: Changing Diabetes in Indonesia. In N. Nordisk, The Blueprint for Change Programme Novo Nordisk: Indonesia. 2013; pp. 3.

  • Nerurkar, P.V., Phoebe W. Hang and Erik Saksa. Anti-Diabetic Potential of Noni : The Yin and the Yang, Molecules. 2015; 20: 17684-17719.

  • Adnyana IK, Elin Yulinah, Andreanus A. Soemardji, Endang Kumolosasi, Maria Immaculata Iwo, Joseph Iskendiarso Sigit, and Suwendar. Antidiabetic Activity of Ethanol Extract Noni Fruit (Morinda citrifolia L.). Acta Pharmaceutica Indonesia. 2004; 29(2): 43-49.

  • Rao, U.S.Mahadeva, and Subramanian, S. Biochemical Evaluation of Antihyperglycemic and Antioxidative Effects of Morinda citrifolia Fruit Extract Studied in Streptozotocin-Induced Diabetic Rats. Medical Chemistry Research. 2008; 18, 433-446.

  • Kustarini, I., Dewi, S.S., and Ika, P.M. Ethanol Extract of Morinda citrifolia L. (Mengkudu), Blood Glucose, Neutrophyl Count, and Glomerulus Fibronectin in Diabetes Mellitus Rat. Media Medika Indonesiana. 2012; 46(3): 178-183.

  • Hadijah, H., Ayub, M.Y., Zaridah, H., and Normah, A. Hypolipidemic Activity of an Aqueous Extract Of Morinda Citrifolia Fruit in Normal and Streptozotocin-Induced Diabetic Rats. Journal of Tropical Agriculture and Food Science. 2008; 36(1): 77-85.

  • Nayak, B. Shivananda, Marshall, Julien R., Isitor, Godwin, Adogwa, Andrew. Hypoglycemic and Hepatoprotective Activity of Fermented Fruit Juice of Morinda citrifolia (Noni) in Diabetic Rats. Evidence-Based Complementary and Alternative Medicine. 2011; 1-5.

  • Ramdhini, N.S. Antidiabetic Activity of Water Fraction, Ethyl acetate and n-hexane of Noni Fruit (Morinda citrifolia L.) in White Mice. Essay. Faculty of Pharmacy, Padjadjaran University, Indonesia. (2005).

  • Farnsworth, N.R. Biological and Phytochemical Screening of Plants. Journal of Pharmaceutical Sciences. 1966; 55(3): 225-276.

  • Zhou, Z.I., L. Jing, G. Cui, Q. Feng and Y. Xiao. Effect of polysaccharide from Lycium barbarum in Alloxan-Induced Diabetic Mice. African J. Biotech. 2009; 8(23): 6634-6637.

  • Diniz, S.F., Amorim, F.F. Cavalcante-Neto, A.L. Bocca, A.C. Batista, G.E.P.M. Simm and T.A. Silva. Alloxan Induced Diabetes Delays Repair in a Rat Model of Closed Tibial Fracture. Brazilian J. Med. Biol. Res. 2008; 41:373-379.

  • Goodman Gilman, A., Hardman, J. G., and Limbird, L. E. (Eds.). Goodman & Gilman’s the Pharmacological Basis of Therapeutics, 10th ed., McGraw-Hill, New York. 2001; pp. 1701.

  • Harvey, R.A. and Champe, P.C. Lippincott’s Illustrated Reviews: Pharmacology, Lippincott-Raven, New York, 1992; pp. 260.

  • Heinicke RM. The Pharmacologically Active Ingredient of Noni. Pacific Tropical Botanical Garden Bulletin. 1985; 15:10-14.

  • Zega VL, Pemsi M. Wowor, Christi Mambo. Test multiple doses of Morinda citrifolia Extract on Blood Glucose Levels in Alloxaninduced Wistar Rats (Rattus norvegicus). Journal e-Biomedik (eBm). 2016; 4(2): 1-5.


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  • Hypoglycemic Activity of Ethyl Acetate Fraction of Mengkudu Fruit (Morinda citrifolia L.) in Mice Induced Alloxan

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Authors

Asman Sadino
Department of Pharmacology and Pharmacy Clinic, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, Indonesia
Ahmad Muhtadi
Department of Pharmacology and Pharmacy Clinic, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, Indonesia
Yasmiwar Susilawati
Department of Pharmaceutical Biology, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, Indonesia
Shintia Lintang Charisma
Department of Pharmaceutical Biology, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, Indonesia

Abstract


Complementary medicine for diabetes mellitus using medicinal plants is the solution chosen as an alternative treatment. Mengkudu fruit (Morinda citrifolia L.) is empirically used by the community for the treatment of diabetes mellitus. To study the hypoglycemic activity of subfraction mengkudu fruit in mice induced alloxan. The test results on subfraction of ethyl acetate fraction (dose 150 mg/kgBW) induced alloxan (dose 175 mg/kgBW) showed that there was a decrease in blood glucose levels including Mc-IIb (33.94%), Mc-IIc (32.21%), Mc-IIa (29.58%), Mc-IId (18.16%) and Mc-IIe (8.09%) were significantly different compared to the negative control (p<0.05). Subfraction Mc-IIb shows that the most active hypoglycemic activity may be due to some secondary metabolites such as flavonoids, saponins, alkaloids, and polyphenols as antidiabetic bioactive principles. These research showed that Mc-IIb subfraction indicates the highest hypoglycemic activity. However, the research for the active hypoglycemic compound of Mc-IIb subfraction needs to be explored further.

Keywords


Morinda citrifolia, Ethyl Acetate Fraction, Subfraction, Hypoglycemic, Alloxan.

References