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Chromatographic Analysis of Famotidine, Paracetamol and Ibuprofen from Tablet Formulation


Affiliations
1 MUP’s College of Pharmacy (B.Pharm), Degaon 444506, Dist. Washim, M.S., India
     

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Aim of the present work was to develop Reversed phase HPLC method for simultaneous estimation of famotidine (FAM), paracetamol (PCT) and ibuprofen (IBU)in bulk drug and tablet formulation. Separation was carried out on Nucleosil C-18 (15cm, 4.6 mm I.D × 250 mm) using a mobile phase methanol and water (ophosphoric acid, 0.05%) (83:17, v/v) at a flow rate of 0.7 ml/min. The detection wavelength was set at 270 nm. Retention time of FAM, PCT and IBU were found to be 1.81, 2.58 and 8.58 min respectively. Linear relationship was found in the concentration range of 0.5-3 μg/ml for FAM (r2 = 0.999), 5-25 μg/ml for PCT (r2 = 0.999) and 5–30 μg/ml for IBU (r2 = 0.999). The % purity was found to be 98.40±1.49, 99.86±0.27 and 100.31±0.76, for FAM, PCT and IBU, respectively. The proposed method was validated as per ICH Q2 (R1) guidelines. The mean % recoveries were found be 100.90±1.99, 99.96±0.97 and 100.51±1.72 for FAM, PCT and IBU, respectively.

Keywords

Famotidine, Paracetamol, Ibuprofen, RP-HPLC, Validation.
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  • Chromatographic Analysis of Famotidine, Paracetamol and Ibuprofen from Tablet Formulation

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Authors

Wrushali A. Panchale
MUP’s College of Pharmacy (B.Pharm), Degaon 444506, Dist. Washim, M.S., India
Rajnandini Suroshe
MUP’s College of Pharmacy (B.Pharm), Degaon 444506, Dist. Washim, M.S., India
Minakshi S. Rathod
MUP’s College of Pharmacy (B.Pharm), Degaon 444506, Dist. Washim, M.S., India
Yuvraj L. Pandhare
MUP’s College of Pharmacy (B.Pharm), Degaon 444506, Dist. Washim, M.S., India

Abstract


Aim of the present work was to develop Reversed phase HPLC method for simultaneous estimation of famotidine (FAM), paracetamol (PCT) and ibuprofen (IBU)in bulk drug and tablet formulation. Separation was carried out on Nucleosil C-18 (15cm, 4.6 mm I.D × 250 mm) using a mobile phase methanol and water (ophosphoric acid, 0.05%) (83:17, v/v) at a flow rate of 0.7 ml/min. The detection wavelength was set at 270 nm. Retention time of FAM, PCT and IBU were found to be 1.81, 2.58 and 8.58 min respectively. Linear relationship was found in the concentration range of 0.5-3 μg/ml for FAM (r2 = 0.999), 5-25 μg/ml for PCT (r2 = 0.999) and 5–30 μg/ml for IBU (r2 = 0.999). The % purity was found to be 98.40±1.49, 99.86±0.27 and 100.31±0.76, for FAM, PCT and IBU, respectively. The proposed method was validated as per ICH Q2 (R1) guidelines. The mean % recoveries were found be 100.90±1.99, 99.96±0.97 and 100.51±1.72 for FAM, PCT and IBU, respectively.

Keywords


Famotidine, Paracetamol, Ibuprofen, RP-HPLC, Validation.

References