Research Journal of Pharmacy and Technology

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Hyper Immune Response against T-2 Mycotoxin in Oryctolagus Cuniculus


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1 Department of Biotechnology, RV College of Engineering, Bangalore, India
     

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Development of standardize protocol for the production of antibodies produced due to hyper immune response in Oryctolagus cuniculus (Rabbit). Rabbits were selected as the ideal model organism because they are easy to bleed subsequently and a better hyper immune response is observed without the death of the organism. The hyper immune response was created against T2 toxin which was diluted; a mixture was made with Freund’s adjuvant (complete and incomplete) and injected into two rabbits kept under observation. One booster dose was given on the 7th day. The injection was given at three different sites and the rabbits were kept for observation. The rabbits were bleeding on 7th, 14th, 21st and 28th day and serum was separated from blood. The serum collected was analyzed for antigen antibody reaction using ELISA. From these studies we have concluded that at 1:100 serum dilutions and 1:50 antigen dilution the antibody-antigen reaction is the highest. It can be further concluded that at the high binding affinity of the toxin to the immobilized antibody, which is evident by the high binding and the low dissociation constant values obtained. This hyper immune serum consists of polyclonal antibodies that can be used for the development of various kit based immunosensors.

Keywords

Rabbit, T-2 Mycotoxin, Hyperimmune Response, Antibody and ELISA.
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  • Hyper Immune Response against T-2 Mycotoxin in Oryctolagus Cuniculus

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Authors

S. Narendra Kumar
Department of Biotechnology, RV College of Engineering, Bangalore, India
Lingayya Hiremath
Department of Biotechnology, RV College of Engineering, Bangalore, India
Praveen Kumar Gupta
Department of Biotechnology, RV College of Engineering, Bangalore, India
Ajeet Kumar Srivastava
Department of Biotechnology, RV College of Engineering, Bangalore, India
Akanksha Jain
Department of Biotechnology, RV College of Engineering, Bangalore, India
Abhishek R. Hegde
Department of Biotechnology, RV College of Engineering, Bangalore, India
L. Hamsa
Department of Biotechnology, RV College of Engineering, Bangalore, India

Abstract


Development of standardize protocol for the production of antibodies produced due to hyper immune response in Oryctolagus cuniculus (Rabbit). Rabbits were selected as the ideal model organism because they are easy to bleed subsequently and a better hyper immune response is observed without the death of the organism. The hyper immune response was created against T2 toxin which was diluted; a mixture was made with Freund’s adjuvant (complete and incomplete) and injected into two rabbits kept under observation. One booster dose was given on the 7th day. The injection was given at three different sites and the rabbits were kept for observation. The rabbits were bleeding on 7th, 14th, 21st and 28th day and serum was separated from blood. The serum collected was analyzed for antigen antibody reaction using ELISA. From these studies we have concluded that at 1:100 serum dilutions and 1:50 antigen dilution the antibody-antigen reaction is the highest. It can be further concluded that at the high binding affinity of the toxin to the immobilized antibody, which is evident by the high binding and the low dissociation constant values obtained. This hyper immune serum consists of polyclonal antibodies that can be used for the development of various kit based immunosensors.

Keywords


Rabbit, T-2 Mycotoxin, Hyperimmune Response, Antibody and ELISA.

References