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Comparative study of Probiotic Bacteria on ornamental fish giant gourami, Osphronemus goramy for its survival and growth


Affiliations
1 PG & Research Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, India
2 PG & Research Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, India
3 Universiti Sultan ZainalAbidin, Terengganu, Malaysia
     

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Production of high quality giant gourami is considered essential to prevent the disease outbreak, survival and growth at aquarium. This paper deals with the production of high quality giant gourami, Osphronemus goramy using probiotics. Materials and Methods: This experiment was designed in such way that stocking the Giant gourami in fourgroups, the first one as control without probiotics and the second as experimental with Bacillus sp, third with Lactobacillus spand the fourth as consortium; a mixture of Bacillus sp and Lactobacillus sp. Results and Discussion: The results were compared in terms of quality of Giant gourami. The fish treated with consortium exhibited highest growth rate (Body Weight-4.13+8.24 gm) and survival of 95% at Total Length– 4.23+8.47 cm stage. The upper most growth rate was noted in group IV treated daily with consortium. This treatment group improved in mean weight from 4.13 to 8.27 with an average growth rate of 0.069% per day. This was significantly better than the control group I which grew from 4.15 to 5.83 g with an average of 0.028 % per day. In the experimental tanks the water treated with probiotics played a key role in maintaining the water quality parameters and keeping the ammonia level less than 1.0 ppm. Conclusion: It was established that the use of probiotics plays a very important role in maintaining the water quality, survival and growth rate in ornamental fish forming systems.

Keywords

Bacillus sp, consortium, Giant gourami, Lactobacillus sp, Osphronemus goramy, Probiotics.
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  • Avella, M.A., G. Gioacchini, O. Decamp, P. Makridis, C. Bracciatelli and O. Carnevali. 2010. Application of multi-species of Bacillus in sea bream larviculture. Aquaculture, 305: 12-19.
  • Taoka, Y., H. Maeda, J. Jo, M. Jeon, S.C. Bai, W. Lee, K. Yuge and S. Koshio. 2006. Growth, stress tolerance and non-specific immune response of Japanese flounderParalichthysolivaceus to probiotics in a closed recirculating system. Fish. Sci., 72: 310-321.
  • Fuller, R. 1989. A review : Probiotics in man and animals. J. Appl. Bacteriol. 66 : 365-378.
  • Tannock, G.W., 1997. Control of gastrointestinal pathogens by normal flora. In Klug, M.J., Reddy, C.A., (Ed), Current perspectives in microbial biology. American Society for Microbiology, Washingtion, DC, pp. 374 – 382.
  • Velmurugan, S. and S. Rajagopal. 2009. Beneficial uses of probiotics in mass scale production of marine ornamental fish. Afr. J. Microbiol. Res., 3: 185-190.
  • Aly, S.M., Y.A. Ahmed, A.A. Ghareeband M.F. Mohamed. 2008. Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromisniloticus) to challenge infections. Fish Shellfish Immunol., 25: 128-136.
  • Rollo, A., R. Sulpizio, M. Nardi, S. Silvi, C. Orpianesi, M. Caggiano, A. Cresci and O. Carnevali. 2006. Live microbial feed supplement in aquaculture for improvement of stress tolerance. Fish Physiol. Biochem., 32: 167-177.
  • Picchietti, S., A.M. Fausto, E. Randelli, O. Carnevali, A.R. Taddei, F. Buonocore, G. Scapgloatiand L. Abelli. 2009. Early treatment with Lactobacillus delbrueckii strain induces an increase in intestinal T-cells and granulocytes and modulates immunerelated genes of larval Dicentrarchuslabrax (L.). Fish Shellfish Immunol., 26: 368-376.
  • Cha, J.H., S. Rahimnejad, S.Y. Yang, K.W. Kim and K.J. Lee. 2013. Evaluations of Bacillus spp. as dietary additives on growth performance, innate immunity and disease resistance of olive flounder (Paralichthysolivaceus) against Streptococcus iniae and as water additives. Aquaculture, 402-403: 50-57.
  • Silvi, S., M. Nardi, R. Sulpizio, C. Orpianesi, M. Caggiano, O. Carnevali and A. Cresci. 2008. Effects of addition of Lactobacillus delbrueckii sp. on gut microbiota composition and contribution to the well-being of the European sea bass (Dicentrarchuslabrax L.). Microbial Ecol. Health Dis., 20: 53-59.
  • Lazado, C.C and C.M.A. Caipang. 2014. Mucosal immunity and probiotics in fish. Fish Shellfish Immunol., 39: 78-89.
  • Picchietti, S., M. Mazzini, A.R. Taddei, R. Renna, A.M. Fausto, V. Mulero, O. Carnevali, A. Cresciand L. Abelli. 2007. Effects of administration of probiotic strains on GALT of larval gilthead seabream: immune histochemical and ultrastructural studies. Fish Shellfish Immunol., 22: 57-67.
  • Rana, K.J. 1997. Status of global productionand production trends, p. 3-16. In Review of the state of the world aquaculture. FAO Fisheries circular no. 886. Food and Agriculture Organization of the United Nations, Rome, Italy.
  • Subasinghe, R. 1997. Fish health and quarantine, P. 45 – 49.In Review of the State of the World Aquaculture.FAO Fisheries circular no. 886.Food and Agriculture Organization of the United Nations, Rome, Italy.
  • Decamp, O., makridis, P., Vercauteren , T., Diereckens, K., and K. Van Driessche, 2004. Selection of probiotics for marine larviculture, Global aquaculture advocate, 38 – 40.
  • Laxmi, P., Dharmendra, S., Baghel and K. Vikas, 2005.Role and prospect of probiotics use in aquaculture. Aquaculture 4 (2): 247 – 251.
  • Kim, H. and G. Lone, 2003. Elucidation of the Vibrio anguillaraum Genetic Response of the potential fish probiontPseudomonas fluorescens, AH2 using RNA Arbitrarily primed PCR. Journal of Bacteriology 183 (3): 831 – 842.
  • Holt, J.G., N.R., Krieg, P.H.A Sneathm, J.T. Staley and S.T. Williams,(1994) Bergey’s Manual of Determinative Bacteriology, 9thedn.Baltimore, MD: Williams and Williams.
  • Strickland, J. D. H. and Parsons, T. R. (1972). A Practical Handbook of Seawater Analysis, London: Bioscience Publishers Inc.
  • Hach, C. C. Klein, Jr., R. L. and Gibbs, C. R. (1992). Introduction to biochemical oxygen demand (Technical information series – Booklet 7), Hach Technical Center for Applied Analytical Chemistry, Hach Company, Loveland, Colorado, USA.
  • J. F. Queiroz and C. E. Boyd, “Effects of a bacterial inoculums in channel catfish ponds,” Journal of the World Aquaculture Society, vol. 29, no. 1, pp. 67–73, 1998.
  • L. Jiqiu, T. Beiping, M. Kangsen et al., “Comparative study between probiotic bacterium Arthrobacter XE-7 and chloramphenicol on protection of Penaeuschinensis post larvae from pathogenic Vibrios,” Aquaculture, vol. 253, no.1–4, pp. 140–147, 2006.
  • Gatesoupe, F. J. 1999. Lactic acid bacteria increase the resistance of turbot larvae, Scophthalmusmaximus, against pathogenic Vibrio. Aquat. Living Resour.7: 277-282.
  • Austin, B, Stuckey, L.F., Robertson, P.A.W., Effendi, I., Griffith, D.R.W. 1995. A probiotic strain of Vibrio alginolyticus effective in reducing diseases caused by Aeromonassalmonicida, Vibrio anguillarum and Vibrio ordalii. Journal of fish diseases 18: 93-96.
  • Gibson, L., J. Woodworth, and A. George. 1998. Probiotic activity of Aeromonas media on the Pacific oyster, Crassostreagigas, when challenged with Vibrio tubiashii. Aquaculture 169 : 111120.
  • Giri S. S., Sen S. S., Sukumaran V. (2012). Effects of dietary supplementation of potential probiotic Pseudomonas aeruginosa VSG-2 on the innate immunity and disease resistance of tropical freshwater fish, Labeorohita. Fish Shellfish Immun. 32, 1135– 1140
  • Sun, Y.Z., H.L. Yang, R.L. Ma, C.X. Zhang and W.Y. Lin, 2011. Effect of dietary administration of Psychrobacter sp. on the growth, feed utilization, digestive enzymes and immune responses of grouper Epinepheluscoioides. Aquaculture Nutrition, 17:733740.
  • Suzer, C., D. Coban, H.O. Kamaci, S. Saka, K. Firat, O. Otgucuoglu and H. Kucuksari, 2011. Lactobacillus spp. bacteria as probiotics in gilthead sea bream (Sparusaurata, L.) larvae: Effects on growth performance and digestive enzyme activities. Aquaculture, 280:140-145
  • Olsson, J.C., A. Westerdahl, P. Conway, and S. Kjelleberg. 1992. Intestinal colonization potential of turbot (Scophthalmusmaximus) and dab (Limandalimanda) associated bacteria with inhibitory effects against Vibrio anguillarum. Appl. Environ. Microbiol. 58: 551-556.
  • Tannock, G.W., 1983. Control of gastrointestinal pathogens by normal flora. In Klug, M.J., Reddy, C.A., (Ed), Current perspectives in microbial biology. American Society for Microbiology, Washington, DC, pp. 374 – 382.
  • Ringo, E., and F.J. Gatesoupe, 1998. Lactic acid bacteria in fish: a review Aquaculture, 169 : 177 – 203.
  • Noh, S.H. Han, K., Won. T.H., Choi, Y.J. 1994. Effect f antibiotics, enzyme, yeast culture and probiotics on growth performance of israeli carp. Korean Journal of Animal Science.,36 : 480-486.
  • Bogut, I., Milakovic, Z., Bukvic, Z., Brkic, S., Zimmer, R., 1998. Influence of probiotic (Streptococcus faecium M74) on growth and content of intestinalmicroflora in carp (Cyprinuscarpio). Journal of Animal Science., 43: 231 – 235.
  • Fuller, R., 1992. Problems and prospects. In: Fuller, R. (Ed), Probiotics. The Scientific basis. Chapman & Hall, London, UK, pp. 377 – 386.
  • Olafsen, J.A., 2001. Interraction between fish larvae and bacteria in marine aquaculture. Aquaculture 200: 223 – 247.
  • Guzmán-Villanueva, L.T., D. Tovar-Ramírez, E. Gisbert, H. Cordero, F.A. Guardiola, A. Cuesta, J. Meseguer, F. AscencioValle & M.A. Esteban. 2014. Dietary administration of ß-1,3/1,6glucan and probiotic strain Shewanellaputrefaciens, single or combined, on gilthead seabream growth, immune responses and gene expression. Fish Shellfish Immunol., 39: 34-41.
  • Garrido-Pereira, M.A.R., A.L. Braga, A.F. Rocha, L.A. Sampaio& P.C. Abreu. 2013. Effect of ultraviolet (UV) radiation on the abundance and respiration rates of probiotic bacteria. Aquacult. Res., 44(2): 261-267.

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  • Comparative study of Probiotic Bacteria on ornamental fish giant gourami, Osphronemus goramy for its survival and growth

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Authors

S. Illanjiam
PG & Research Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, India
J. Sivakumar
PG & Research Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, India
C. Shanmuga Sundaram
PG & Research Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, India
U. S. Mahadeva Rao
Universiti Sultan ZainalAbidin, Terengganu, Malaysia

Abstract


Production of high quality giant gourami is considered essential to prevent the disease outbreak, survival and growth at aquarium. This paper deals with the production of high quality giant gourami, Osphronemus goramy using probiotics. Materials and Methods: This experiment was designed in such way that stocking the Giant gourami in fourgroups, the first one as control without probiotics and the second as experimental with Bacillus sp, third with Lactobacillus spand the fourth as consortium; a mixture of Bacillus sp and Lactobacillus sp. Results and Discussion: The results were compared in terms of quality of Giant gourami. The fish treated with consortium exhibited highest growth rate (Body Weight-4.13+8.24 gm) and survival of 95% at Total Length– 4.23+8.47 cm stage. The upper most growth rate was noted in group IV treated daily with consortium. This treatment group improved in mean weight from 4.13 to 8.27 with an average growth rate of 0.069% per day. This was significantly better than the control group I which grew from 4.15 to 5.83 g with an average of 0.028 % per day. In the experimental tanks the water treated with probiotics played a key role in maintaining the water quality parameters and keeping the ammonia level less than 1.0 ppm. Conclusion: It was established that the use of probiotics plays a very important role in maintaining the water quality, survival and growth rate in ornamental fish forming systems.

Keywords


Bacillus sp, consortium, Giant gourami, Lactobacillus sp, Osphronemus goramy, Probiotics.

References