Research Journal of Pharmacy and Technology

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Hepato-Protective Studies of Poloxamer – 188 by Paracetomol Induced Liver Toxicity in Rats


Affiliations
1 Dept. of Pharmacology, MAK College of Pharmacy, Moinabad, Hyderabad, Telanagana, 501504, India
2 Dept. of Pharmacology, Pulla Reddy Institute of Pharmacy, Sangareddy, Hyderabad, Telanagana 502313, India
     

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Poloxamer-188 (P-188) is a polymer well renowned for possessing medical properties. Present study was conducted with an aim to evaluate Hepato-protective potential of P-188 against paracetamol induced liver damage in wistar albino rats. Group-I served as Normal control which received normal saline 5ml/kg of Body weight (BW) for 7 days. Group-II served as Negative control in which animals received same dose as mentioned in Group-I. Group-III, IV and V animals received P-188(50mg/kg), P-188(100mg/kg) and silymarin (25mg/kg) respectively for 7 days. Except Group-I all other groups challenged with a very high dose of paracetamol (750 mg/kg) to induce hepatotoxicity. Biochemical estimation results showed that SGOT, SGPT and ALP levels were increased in negative control group animals which were decreased to normal levels when treated with P-188. There was highly significant (p<0.0001) decrease of these values in all three treatment groups. Moreover decrease in P-188 treated groups is dose dependent. Histopathological study supports the results of biochemical estimation. Negative control animal showed inflammation with mild cholestasis. P-188 (50mg/kg) showed mild inflammation, more number of normal hepatocytes, whereas P-188 (100 mg/kg) showed scattered lymphocytes among normal hepatocytes and kupffer cell hyperplasia, no incidence of inflammation and necrosis. Based on these results we can conclude that P-188 is a hepatoprotective polymer.

Keywords

Poloxamer-188, Paracetamol, Hepato-Protective, Silymarin, Histopathological Study.
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  • Hepato-Protective Studies of Poloxamer – 188 by Paracetomol Induced Liver Toxicity in Rats

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Authors

T. Arif Hussain
Dept. of Pharmacology, MAK College of Pharmacy, Moinabad, Hyderabad, Telanagana, 501504, India
M. D. Imad Uddin
Dept. of Pharmacology, Pulla Reddy Institute of Pharmacy, Sangareddy, Hyderabad, Telanagana 502313, India
M. D. Irfan Uddin
Dept. of Pharmacology, MAK College of Pharmacy, Moinabad, Hyderabad, Telanagana, 501504, India
M. D. Nadeem
Dept. of Pharmacology, MAK College of Pharmacy, Moinabad, Hyderabad, Telanagana, 501504, India
H. Talha
Dept. of Pharmacology, MAK College of Pharmacy, Moinabad, Hyderabad, Telanagana, 501504, India
K. Prashanth
Dept. of Pharmacology, MAK College of Pharmacy, Moinabad, Hyderabad, Telanagana, 501504, India

Abstract


Poloxamer-188 (P-188) is a polymer well renowned for possessing medical properties. Present study was conducted with an aim to evaluate Hepato-protective potential of P-188 against paracetamol induced liver damage in wistar albino rats. Group-I served as Normal control which received normal saline 5ml/kg of Body weight (BW) for 7 days. Group-II served as Negative control in which animals received same dose as mentioned in Group-I. Group-III, IV and V animals received P-188(50mg/kg), P-188(100mg/kg) and silymarin (25mg/kg) respectively for 7 days. Except Group-I all other groups challenged with a very high dose of paracetamol (750 mg/kg) to induce hepatotoxicity. Biochemical estimation results showed that SGOT, SGPT and ALP levels were increased in negative control group animals which were decreased to normal levels when treated with P-188. There was highly significant (p<0.0001) decrease of these values in all three treatment groups. Moreover decrease in P-188 treated groups is dose dependent. Histopathological study supports the results of biochemical estimation. Negative control animal showed inflammation with mild cholestasis. P-188 (50mg/kg) showed mild inflammation, more number of normal hepatocytes, whereas P-188 (100 mg/kg) showed scattered lymphocytes among normal hepatocytes and kupffer cell hyperplasia, no incidence of inflammation and necrosis. Based on these results we can conclude that P-188 is a hepatoprotective polymer.

Keywords


Poloxamer-188, Paracetamol, Hepato-Protective, Silymarin, Histopathological Study.

References