Research Journal of Pharmacy and Technology

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A New Stability- Indicating RP-HPLC- Pda Method for Simultaneous Estimation of Glecaprevir and Pibrentasvir in Tablet Dosage Form


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1 Department of Chemistry, Sri Krishnadevaraya University, Ananthapuramu-515003 A.P, India
     

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A simple, accurate, precise method was developed for the simultaneous estimation of the Glecaprevir and Pibrentasvir in tablet dosage form. Chromatogram was run through Std Zodiac 150 x 4.6 mm, 5. Mobile phase containing buffer 0.1% OPA: Acetonitrile taken in the ratio 55:45 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 260 nm. Retention time of Glecaprevir and Pibrentasvir were found to be 2.207 min and 3.263 min. %RSD of the Glecaprevir and Pibrentasvir were and found to be 0.4 and 0.3 respectively. %Recovery was obtained as 98.90% and 99.61% for Glecaprevir and Pibrentasvir respectively. LOD, LOQ values obtained from regression equations of Glecaprevir and Pibrentasvir were 0.32, 0.97 and 0.04, 0.11 respectively. Regression equation of Glecaprevir is y= 23713.x + 11307, y = 13524x +2221 of Pibrentasvir. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular quality control test in industries.

Keywords

Glecaprevir, Pibrentasvir, RP-HPLC Method Development, Validation.
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  • A New Stability- Indicating RP-HPLC- Pda Method for Simultaneous Estimation of Glecaprevir and Pibrentasvir in Tablet Dosage Form

Abstract Views: 255  |  PDF Views: 0

Authors

Yeragodala Narendra Reddy
Department of Chemistry, Sri Krishnadevaraya University, Ananthapuramu-515003 A.P, India
Jadi Sreeramulu
Department of Chemistry, Sri Krishnadevaraya University, Ananthapuramu-515003 A.P, India
B. Balaswami
Department of Chemistry, Sri Krishnadevaraya University, Ananthapuramu-515003 A.P, India

Abstract


A simple, accurate, precise method was developed for the simultaneous estimation of the Glecaprevir and Pibrentasvir in tablet dosage form. Chromatogram was run through Std Zodiac 150 x 4.6 mm, 5. Mobile phase containing buffer 0.1% OPA: Acetonitrile taken in the ratio 55:45 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 260 nm. Retention time of Glecaprevir and Pibrentasvir were found to be 2.207 min and 3.263 min. %RSD of the Glecaprevir and Pibrentasvir were and found to be 0.4 and 0.3 respectively. %Recovery was obtained as 98.90% and 99.61% for Glecaprevir and Pibrentasvir respectively. LOD, LOQ values obtained from regression equations of Glecaprevir and Pibrentasvir were 0.32, 0.97 and 0.04, 0.11 respectively. Regression equation of Glecaprevir is y= 23713.x + 11307, y = 13524x +2221 of Pibrentasvir. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular quality control test in industries.

Keywords


Glecaprevir, Pibrentasvir, RP-HPLC Method Development, Validation.

References