Stability Indicating HPLC-DAD Method for the Determination of Oxcarbazepine-An Anticonvulsant

Oxcarbazepine is an anticonvulsant drug used for the treatment of epilepsy. It acts by decreasing the abnormal electrical activity in the brain. A new stability indicating HPLC method was developed for the quantification of Oxcarbazepine in tablets. Waters e2695 Separations module with Waters 2489 UV detector with Hypersil BDS C18 column (250 mm x 4.6 mm, 5μ) was operated with column temperature 50°C. The detector was monitored at 215 nm and the chromatographic study was run for 20 min. Linearity was observed over 5.0-150 μg/mL with linear regression equation y = 47,910x + 6493.8 with correlation coefficient R² = 0.9999. The LOQ and LOD were found to be 4.5129 μg/mL and 1.4898 μg/mL respectively. Forced degradation studies were performed for Oxcarbazepine capsules and the method was validated as per ICH guidelines.