Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Poly Herbal Hair Oil Preparation, Standardization, Treatment and Evaluation for Alopecia in Male Wistar Rats


Affiliations
1 Department of Pharmacy, Pranveer Singh Institute of Technology, Kanpur, India
     

   Subscribe/Renew Journal


Background: Baldness is very common in males and females but predominantly it is seen more in males. This accounts for approximately 89-99 percent of hair loss. Though there are multiple causes responsible for causing baldness like hormones, hereditary, age factors, pollution, work environment, stress and many more. Objective: The objective of the paper is to evaluate the effectiveness of poly herbal hair oil prepared in the laboratory from sources such as hibiscus, leech, beehive, and onion which could be a potential hair growth promoter. Methods: The hair from the dorsal area of male wistar rats was shaved and they were divided into three groups having 6 rats each. Group I received the application of coconut oil daily, Group II received application of Minodixil (2%) ethanol extract as standard hair growth promoter and Group III received the application of poly-herbal oil prepared in the laboratory. The oil in respective groups was applied daily on rats of each group on the shaved surface. Results: The initiation of hair growth in all groups was calculated as a mean value to compare the results in different groups. Then the length of hair was noted on 15, 21, 30 and 40th day in centimeters. The mean length was calculated on each day for every group and the results were compared. The anagen/telogen ratio was also calculated for all the groups for better evaluation of results. Conclusion: The hair growth was maximum in the case of Group III, on which the poly herbal formulation of oil was applied daily. The hair growth in Group II (Minoxidil) was observed with optimum hair growth while the Group I was observed with least hair growth after the final evaluation and comparison of all the results. A significant hair growth effect from the poly herbal formulation oil was noted from the research conducted.

Keywords

Alopecia, Leech Oil, Hair Loss, Baldness, Poly Herbal Hair Oil, Herbal Extract.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Schmitt J. V., Ribeiro C. F., Souza F. H., Siqueira E. B. and Bebber F. R., Hair Loss Perception and Symptoms of Depression in Female Outpatients Attending a General Dermatology Clinic, Anais Brasileiros de Dermatologia, Vol. 87, No. 3, 2012, pp. 412-417. Doi: 10.1590/S0365-05962012000300010

  • Shrivastava S. B., Diffuse Hair Loss in an Adult Female: Approach to Diagnosis and Management, Indian Journal of Dermatology, Venereology and Leprology, Vol. 75, No. 1, 2009, pp. 20-28. doi:10.4103/0378-6323.45215

  • Chartier M. B., Hoss D. M. and Grant-Kels J. M ., Approach to the Adult Female Patient with Diffuse Nonscar- ring Alopecia, Journal of the American Academy of Dermatology, Vol. 47, No. 6, 2002, pp. 809-818. doi:10.1067/mjd.2002.128771

  • Tosti A., Piraccini B. M., Sisti A. and Duque-Estrada B. Hair Loss in Women, Minerva Ginecologica, Vol. 61, No. 5, 2009, pp. 445-52

  • Hawryluk E. B. and English J. C., Female Adolescent Hair Disorders, Journal of Pediatric and Adolescent Gynecology, Vol. 22, No. 4, 2009, pp. 271-281. doi:10.1016/j.jpag.2009.03.007

  • Mounsey A. L. and Reed S. W., Diagnosing and Treat- ing Hair Loss, American Family Physician, Vol. 80, No. 4, 2009, pp. 356-362.

  • Shapiro J., Wiseman M., Lui H. Practical Management of Hair Loss, Canadian Family Physician, Vol. 46, No. 7, 2000, pp. 1469-1477.

  • Mulinari-Brenner F. and Bergfeld W. F., Hair Loss: An Overview, Dermatology Nursing, Vol. 13, No. 4, 2001, pp. 269-272, 277-278.

  • Franca K, Rodrigues TS, Ledon J, Savas J, Chacon A. Comprehensive overview and treatment update on hair loss. J Cosmet Dermatol Sci Appl. 2013; 3:1–8.-R

  • Upadhyay S, Ghosh AK, Singh V. Hair growth promotant activity of petroleum ether extract of Glycyrrhiza Glabra L (Fabaceae) in female rats. Trop J Pharm Res. 2012; 11:753–8.-R

  • Sharquie KE, Al-Obaidi HK. Onion juice (Allium cepa L.), a new topical treatment for alopecia areata.J Dermatol. 2002; 29(6):343-6.-R

  • Hillmer, A.M. et al.: Genetic Variation in the Human Androgen Receptor Gene Is the Major Determinant of Common Early Onset Androgenetic Alopecia, Am. J. Hum. Genet. 77 (2001):140–148.-R

  • Trueb RM: Oxidative stress in ageing of hair. International Journal of Trichology 2009; 1(1)

  • Koh CY and Kini RM. Anticoagulants from hematophagous animals. Expert Rev Hematol. 2008; 1:135–9.

  • Faria F, Kelen EM, Sampaio CA, Bon C, Duval N, Chudzinski-Tavassi AM. A new factor Xa inhibitor (lefaxin) from the Haementeria depressa leech. Thromb Haemost. 1999; 82:1469–73.

  • Whitaker IS, Rao J, Izadi D, Butler PE. Historical article: Hirudo medicinalis: Ancient origins of, and trends in the use of medicinal leeches throughout history. Br J Oral Maxillofac Surg. 2004; 42:133–7.

  • Smith DB. On the medicinal leeches. J Phila Coll Pharm. 1833; 4:265–71.

  • Moore JP. Professor A.E. Verrill's fresh-water leeches-A tribute and a critique. Acad Nat Sci Phila. 1952; 245:1–16.

  • Kim DR, Kang KW. Amino acid sequence of piguamerin, an antistasin-type protease inhibitor from the blood sucking leech Hirudo nipponia. Eur J Biochem. 1998; 254:692–7.

  • Jung HI, Kim SI, Ha KS, Joe CO, Kang KW. Isolation and characterization of guamerin, a new human leukocyte elastase inhibitor from Hirudo nipponia. J Biol Chem. 1995; 270:13879–84.

  • Chudzinski-Tavassi AM, Kelen EM, de Paula Rosa AP, Loyau S, Sampaio CA, Bon C, et al. Fibrino (geno) lytic properties of purified hementerin, a metalloproteinase from the leech Haementeria depressa. Thromb Haemost. 1998; 80:155–60.

  • Electricwala A, Sawyer RT, Jones CP, Atkinson T. Isolation of thrombin inhibitor from the leech Hirudinaria manillensis. Blood Coagul Fibrinolysis. 1991; 2:83–9.

  • Baskova IP, Kostrjukova ES, Vlasova MA, Kharitonova OV, Levitskiy SA, Zavalova LL, et al. Proteins and peptides of the salivary gland secretion of medicinal leeches Hirudo verbana, H. medicinalis, and H. orientalis. Biochemistry (Mosc) 2008; 73:315–20.

  • Maton A. New Jersey: Prentice Hall; 1993. Human Biology and Health.

  • Corral-Rodríguez MA, Macedo-Ribeiro S, Pereira PJ, Fuentes-Prior P. Leech-derived thrombin inhibitors: From structures to mechanisms to clinical applications. J Med Chem. 2010; 53:3847–61.

  • Haycraft JB. On the action of a secretion obtained from the medicinal leech on the coagulation of the blood. Proc R Soc Lond. 1883; 36:478–87.

  • Markwardt F. Hirudin as an inhibitor of thrombin. Methods Enzymol. 1970; 19:924–32.

  • Conforti ML, Connor NP, Heisey DM, Vanderby R, Kunz D, Hartig GK. Development of a mechanical device to replace medicinal leech (Hirudo medicinalis) for treatment of venous congestion. J Rehabil Res Dev. 2002; 39:497–504

  • Walsmann P and Markwardt F. On the isolation of the thrombin inhibitor hirudin. Thromb Res. 1985; 40:563–9.

  • Markwardt F. Historical perspective of the development of thrombin inhibitors. Pathophysiol Haemost Thromb. 2002; 32(Suppl 3):15–22.

  • Strube KH, Kröger B, Bialojan S, Otte M, Dodt J. Isolation, sequence analysis, and cloning of haemadin. An anticoagulant peptide from the Indian leech. J Biol Chem. 1993; 268:8590–5.

  • Hong SJ and Kang KW. Purification of granulin-like polypeptide from the blood-sucking leech, Hirudo nipponia. Protein Expr Purif. 1999; 16:340–6.

  • Salzet M, Chopin V, Baert J, Matias I, Malecha J. Theromin, A novel leech thrombin inhibitor. J Biol Chem. 2000; 275:30774–80.

  • 34.. Baskova IP, Korostelev AN, Chirkova LD, Zavalova LL, Basanova AV, Doutremepuich C. Piyavit from the medicinal leech is a new orally active anticoagulating and antithrombotic drug. Clin Appl Thromb Hemost. 1997; 3:40–5.

  • Cherniack EP. Bugs as drugs, part two: Worms, leeches, scorpions, snails, ticks, centipedes, and spiders. Altern Med Rev. 2011; 16:50–8.

  • Michalsen A, Roth M, Dobos G, Aurich M. Stattgurt, Germany: Apple Wemding; 2007. Medicinal Leech Therapy.

  • Upadhyay S, Upadhyay P. Hibiscus rosa-sinensis: pharmacological review. International Journal of Research in Pharmaceutical and Biomedical Sciences 2011; 2: 1449-1450

  • Upadhyay S, Upadhyay P, Vinode R, Dixit VK. Effect of ethanolic fraction of Hibiscus rosa sinensis l., leaves in androgenic alopecia. Egyptian Dermatology Online Journal 2013; 9: 2.

  • Adhirajan N, Kumar TR, Shanmugasundaram N, Babu M. In vivo and in vitro evaluation of hair growth potential of Hibiscus rosa-sinensis Linn. Journal of ethnopharmacology 2003; 88: 235-239.

  • Vyjayanthi G, Kulkarni C, Abraham A, Kolhapure SA. Evaluation of antidandruff activity and safety of polyherbal hair oil: An open pilot clinical trial. The Antiseptic 2004; 101: 368-372

  • Lim TK. Edible Medicinal and Non Medicinal Plants. 1st edition. Springer, Netherlands2014; 8: 309-310

  • Park Y, Kim Y, Han J (2014) Synthesis and Rat Prostate 5-Alpha Reductase Inhibitions of Methylated Quercetin Derivatives. KKU Res 19: 168-71.

  • Brandon B (2014) Apple Cider Vinegar for Health: 100 Amazing and Unexpected Uses for Apple Cider Vinegar. 1st edition, Adams Media, USA, p: 105.

  • Thorat RM, Jadhav VM, Kadam VJ, Kamble SS, Salaskar KP.Development of HPTLC method for estimation of Wedelolactone, Quercetin and Jatamansone in Polyherbal Formulation. International Journal of ChemTech Research2009; 1:1079-86.

  • Kaushik R, Gupta D, Yadav R. ALOPECIA: HERBAL REMEDIES. IJPSR, 2011; Vol. 2(7): 1631-1637.

  • Pal RS, Pal Y, Wal P. In-House Preparation and Standardization of Herbal Face Pack. The Open Dermatology Journal. 2017, volume 11: 72-80.

  • Cobb C. Can Onion Juice Stop Hair Loss? Healthline. August 10, 2017 [cited 21 May 2018]. Available on /www.healthline.com/health/onion-juice-for-hair.

  • Bleumink E, Doeglas H, Klokk A, Nater J.Allergic Contact Dermatitis to Garlic J Dermatol 1972;87:6–9.

  • Burks JW: Classic aspects of onion and garlic dermatitis in housewives, Ann Allergy, 12: 592, 1954.

  • Burgess JF: Occupational dermatitis to onion and garlic. Can Med Ass J 1952; 66: 235.

  • Dorsch W, Ring J: Suppression of immediate and late anti-IgE induced skin reactions by topically applied alcohol/onion extract.J Allergy 1984; 39: 43–49.

  • Happle R: Antigenic competition as a therapeutic concept for alopecia areata, Arch Dermatol Res, 267: 109–114, 1980.

  • WHO monographs on selected medicinal plants, Vol. 1, Bulbus Allii cepa, Geneva, 5–15, 1999.

  • Mursy M, Al Habasha KM, Al-Aziz N (eds): Onion, Anglo Egyptian Comp, Cairo, 1973, pp 21–28.

  • Middleton E: The flavonoids trends in pharmacological sciences (TIPS), 5: 335–338, 1984.

  • Waili NS. Therapeutic and prophylactic effects of crude honey on chronic seborrheic dermatitis and dandruff. Eur J Med Res. 2001; 6(7):306-8.

  • American Chemical Society News Press Pacs 2015 ACS News Service Weekly Press Pac: January 7, 2015 Honeybee hive sealant promotes hair growth in mice FOR IMMEDIATE RELEASE.A PressPac Instant Replay*ACS News Service Weekly PressPac: January 07, 2015.

  • Miyata S, Oda Y, Matsuo C, Kumura H, Kobayashi K. J Agric. Food Chem., 2014, 62 (49), pp 11854–861.

  • Marcucci MC. Propolis: Chemical composition, biological properties and therapeutic activity. J Apidologie. 1995; 26(2):83–99.

  • Yaghoubi SM, Ghorbani GR, Soleimanian-Zade S, Satari R. Antimicrobial activity of Iranian propolis and its chemical composition. Iran Biomed J. 2018 J; 22(1): 50–65.

  • Rebiai A, Lanez T, Belfar ML. Total polyphenol contents, radical scavenging and cyclic voltammetry of Algerian propolis. Int J Pharm Pharm Sci. 2014; 6:395–400.

  • Jastrzebska-Stojko Z, Stojko R, Rzepecka-Stojko A, Kabala-Dzik A, Stojko J. Biological activity of propolis-honey balm in the treatment of experimentally-evoked burn wounds. Molecules. 2013; 18:14397–413.

  • Miyata S, Oda Y, Matsuo C, Kumura H, Kobayashi K. Stimulatory effect of Brazilian propolis on hair growth through proliferation of keratinocytes in mice. J Agric Food Chem. 2014; 62:11854–61.

  • Organic facts. Hair Loss Treatment with Honey, August 30, 2017 by Organic Facts. [cited April 30.2018]. Available from https://www.organicfacts.net/health-benefits/animal-product/hair-loss-treatment-with-honey.

  • Takahashi T1, Kamimura A, Yokoo Y, Honda S, Watanabe Y. The first clinical trial of topical application of procyanidin B-2 to investigate its potential as a hair growing agent.J Phytother Res. 2001;15(4):331-6..

  • Takahashi T, Kamiya T, Hasegawa A, Yokoo Y. Procyanidin oligomers selectively and intensively promote proliferation of mouse hair epithelial cells in vitro and activate hair follicle growth in vivo.J Invest Dermatol. 1999; 112(3):310-6.

  • Takahashi T, Yokoo Y, Inoue T, Ishii A. Toxicological studies on procyanidin B-2 for external application as a hair growing agent. J Food Chem Toxicol. 1999; 37(5):545-52.

  • Rushton DH. DH.Nutritional factors and hair loss. J Clin Exp Dermatol 2002; 27(5):396-404.

  • Prager N, Bickett K, French N, Marcovici G.A randomized, double-blind, placebo-controlled trial to determine the effectiveness of botanically derived inhibitors of 5-alpha-reductase in the treatment of androgenetic alopecia. J Altern Complement Med. 2002; 8(2):143-52.

  • Esfandiari A, Kelly AP. The effects of tea polyphenolic compounds on hair loss among rodents. J Natl Med Assoc 2005; 97(8):1165-9.

  • Claude A., Spreading properties of leech extracts and the formation of lymph, PLATE-9, Laboratories of The Rockefeller Institute for Medical Research, April 15, 1937.

  • Afiune LAF, Leal-Silva T, Sinzato YK, Moraes-Souza RQ, Soares TS, Campos KE, et al. (2017) Beneficial effects of Hibiscus rosa-sinensis L. flower aqueous extract in pregnant rats with diabetes. PLOS ONE | https://doi.org/10.1371/journal.pone.0179785 June 23, 2017.

  • Shinkafi, S. A. and Dauda, H. Antibacterial Activity of Allium Cepa (Onion) On Some Pathogenic Bacteria Associated With Ocular Infections. Sch. J. App. Med. Sci., 2013; 1(3):147-5.

  • Wallis TE, Text book of Pharmacognosy, 5th Edition. New Delhi: CBS Publishers & Distributors, 2002; 123-132, 210-15.

  • Rajpal V.Standardization of Botanicals. Vol. 1. New Delhi: Eastern Publishers; 2002. pp. 39–44.

  • Tandon N, Sharma M. Quality Standards of Indian Medicinal Plants. Vol. New Delhi: Indian Council of Medical Research 2010; 8:pp. 161–3.

  • Ayurvedic Pharmacopoeia of India, Part – 1, Vol – IV, First Edition, Appendix 3 / 1.3.

  • Ayurvedic Pharmacopoeia of India, Part – 1, Vol – IV, First Edition, Appendix 3 / 12.

  • Bureau of Indian standards, BIS Specification, IS 7123:1993.

  • Indian Pharmacopoeia, ministry of health and family welfare, Controller of publications, Govt. of India, volume 1, 1996, A-78.

  • Joshi P,Nanda D,Nainwal P and Saini P, Standardization of herbal ayurvedic oil formulation- Ksheer Bala Taila. Asian Journal of Pharmaceutical Research and Development.2013; 11: 23- 126.

  • Pal RS, Pal Y. A Research on the physicochemical aspects of commonly used Herbal Hair oil preparations. International journal of pharmaceutics & drug analysis V 2016; 4(6): 274 – 275.

  • Ayurvedic Pharmacopoeia of India, Part – 1, Vol – IV, First Edition, Appendix 3 / 10.

  • L. Saraf S, Pathak AK, Dixit VK. Hair growth promoting activity of Tridax procumbens. Fitoterapia 1991; 62: 495-98.


Abstract Views: 247

PDF Views: 0




  • Poly Herbal Hair Oil Preparation, Standardization, Treatment and Evaluation for Alopecia in Male Wistar Rats

Abstract Views: 247  |  PDF Views: 0

Authors

Saraswat Nikita
Department of Pharmacy, Pranveer Singh Institute of Technology, Kanpur, India
Pal S. Rashmi
Department of Pharmacy, Pranveer Singh Institute of Technology, Kanpur, India
Pal Yogendra
Department of Pharmacy, Pranveer Singh Institute of Technology, Kanpur, India
Wal Pranay
Department of Pharmacy, Pranveer Singh Institute of Technology, Kanpur, India
Wal Ankita
Department of Pharmacy, Pranveer Singh Institute of Technology, Kanpur, India
A. K. Rai
Department of Pharmacy, Pranveer Singh Institute of Technology, Kanpur, India

Abstract


Background: Baldness is very common in males and females but predominantly it is seen more in males. This accounts for approximately 89-99 percent of hair loss. Though there are multiple causes responsible for causing baldness like hormones, hereditary, age factors, pollution, work environment, stress and many more. Objective: The objective of the paper is to evaluate the effectiveness of poly herbal hair oil prepared in the laboratory from sources such as hibiscus, leech, beehive, and onion which could be a potential hair growth promoter. Methods: The hair from the dorsal area of male wistar rats was shaved and they were divided into three groups having 6 rats each. Group I received the application of coconut oil daily, Group II received application of Minodixil (2%) ethanol extract as standard hair growth promoter and Group III received the application of poly-herbal oil prepared in the laboratory. The oil in respective groups was applied daily on rats of each group on the shaved surface. Results: The initiation of hair growth in all groups was calculated as a mean value to compare the results in different groups. Then the length of hair was noted on 15, 21, 30 and 40th day in centimeters. The mean length was calculated on each day for every group and the results were compared. The anagen/telogen ratio was also calculated for all the groups for better evaluation of results. Conclusion: The hair growth was maximum in the case of Group III, on which the poly herbal formulation of oil was applied daily. The hair growth in Group II (Minoxidil) was observed with optimum hair growth while the Group I was observed with least hair growth after the final evaluation and comparison of all the results. A significant hair growth effect from the poly herbal formulation oil was noted from the research conducted.

Keywords


Alopecia, Leech Oil, Hair Loss, Baldness, Poly Herbal Hair Oil, Herbal Extract.

References