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Identification of Phytochemical Constituents within the Rhizome Extract of Costusspeciosus (koen) using Gas Chromatography-Mass Spectrometry (GC-MS) Analysis and In vitro Anti Diabetic Activity


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1 Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai-20, India
     

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Costusspeciosus(koen) is widely used in traditional medicine for treatment of cancer and diabetic activity. Both its leaves were reported to possess antioxidant, antibacterial and anti-inflammatory activities. The rhizomes of Costusspeciosus(koen) were extracted with petroleum ether, chloroform, ethyl acetate and methanol by cold maceration extraction procedure and the resultant extract was subjected to phytochemical analyses using GC-MS technique. Three compounds with known biological activities were detected and identified based on GC-MS data as Costunolide. The findings of the study support the usage of Costusspeciosus(koen) in traditional medicinal as most of the identified compounds are known to possess biological activities. The various kinds of phytochemicals were detected and then in vitro antidiabetic activity of Costusspeciosus(koen) methanol extract was detected by using alpha amylase and alpha glucosidase enzyme in an in vitro model. The study reveals the presence of phytochemicals such as carbohydrate, tannin, flavonoids, phenols etc., Among the various extracts the methanol extract which contains the large number of phytoconstituents. The Costusspeciosus(koen) methanol extract has been successfully inhibited both the enzymes in an in vitro model. The methanol extract of Costusspeciosus(koen) inhibited the alpha amylase and alpha glucosidase enzymes as 65.34% and 58.48% respectively. From the result obtained in the current studies, the Costusspeciosus(koen) extract have a prominent antidiabetic property in an in vitro model.

Keywords

Costusspeciosus(koen), Methanol Extract, Phytochemical Constituents, GC-MS, Compound Identification, in vitro Anti Diabetic Activity.
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  • Anonymous. The Wealth of India. Second supplement series (Raw materials). Vol. 2. New Delhi: NISCAIR, CSIR; 2007. p. 211-3.

  • Gupta RK. Medicinal and Aromatic Plants. 1st ed. New Delhi: CBS Publishers and Distributors; 2010. p. 234, 499.

  • Deni B. Encyclopedia of Herbs. London: The Royal Horticulture Society, Dorling Kindersley; 2008. p. 181.

  • Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants. New Delhi: NISCAIR Press; 2006. p. 79.

  • Khare CP. Indian Medicinal Plants. India: Springer (India) Private Limited; 2007. p. 181-2.

  • Nadkarni KM, Nadkarni AK. Indian MateriaMedica. Vol. 1. Mumbai: Bombay Popular Prakashan Pvt. Ltd.; 2007. p. 385-6.

  • Shunmuga jothi, R., Uthayakumari, F., Bharathy, V. Biosci. Discovery 2015, 6, 106-111

  • Oyugi, D.A., Ayorinde, F.O., Gugssa, A., Allen, A., Izevbigie, E.B., Eribo, B., Anderson, W.A. J. Biosci. Tech. 2011, 2, 287-304.

  • Malviya N, Jain S, Malviya S. Antidiabetic potential of medicinal plants. Acta Poloniae Pharm-Drug Res 2010; 67(2):113-118.

  • Shrestha S, Yadav S, Mandal F, Amatya M, Islam MN. Prevalence of diabetes mellitus on male and female patient of different age group on hospital based data of chitwan medical college teaching hospital. J Biomed Pharm Res 2013; 2(4):66-70.

  • Evans JL, Goldfine DI, Maddux AB, and Grodsky MD. Oxidative stress and stress- activated signaling pathways: A unifying hypothesis of type 2 diabetes. Endocrine Reviews 2002; 23(5):599-622.

  • Maritim AC, Sanders RA, Watkins JB. Diabetes, oxidative stress, and antioxidants: A Review. J Biochem Mol Toxicol 2003; 17(1):24-38.

  • Jung M, Park M, Lee HC, Kang YH, Kang ES, Kim SK. Antidiabetic agents from medicinal plants. Curr Med Chem 2006; 13(1):1203–1218.

  • Saxena A, Vikram NK. Role of selected Indian plants in management of type 2 diabetes: a review. J. Alt. Comple Med 2006; 10(1):369-378.

  • Bolen S, Feldman L, Vassy J, Wilson L, Yeh HC, Marinopoulos S. Systematic review: comparative effectiveness and safety of oral medications for type 2 diabetes mellitus. Ann Intern Med 2007; 14(7):386-99.

  • Harborne, Phytochemical Methods, Edition –III, Pub: Springer (India) Private Limited, 2005, p. 1-32.

  • Felipe AMM, Rincao VP, Benati FJ, Linhares REC, Galina KJ, de Toledo CEM, et al. Antiviral effect of Gauzuma ulmifolia and Stryphnodendron adstringens on poliovirus and bovine herpes virus. Biol Pharm Bull. 2006;29(6):1092-5.

  • Srinivasan K, Kumaravel S. Unraveling the Potential Phytochemical Compounds of Gymnema sylvestre through GC-MS Study. Int J Pharm Pharm Sci. 2016;8(1):1-4.

  • Nair SS, Kavrekar V Mishra A. In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts. European Journal of Experimental Biology 2013; 3(1): 128-132.

  • Sudha P, Zinjarde SS, Bhargava SY, Kumar AR. Potent α- amylase inhibitory activity of Indian Ayurvedic medicinal plants. BMC Complementary and Alternative Medicine 2011; (DOI: 10.1186/1472-6882-11-5).

  • Ecobichon DJ. The basis of toxicology testing. New York: RC press; 1997.

  • Tanko Y, Yaro AH, Erima M, Saleh MI, Mohammed A. Toxicological and hypoglycemic studies on the leaves of Cissampelos Mucronata (Menispermaceae) on blood glucose levels of streptozocin –induced diabetic wistar rats. Journal of medicinal plants research 2007; 1(5):113-116.

  • Latha M, Pari L, Sitasawad S, Bhonde R. Insulin secretagogue activity and cytoprotective role of traditional antidiabetic plant Scoparia dulcis. Life Science 2004; 75(1): 203-14.

  • Lefebvre P. Endocrine diseases and diabetes mellitus. Diabetes 2005; 22(1): 25-35.

  • Saxena A. Kishore V. Role of selected medicinal Indian plants in management of type 2 Diabetes: A review. Journal of Alternative and Complementary Medicine 2004; 10(1): 369-378.

  • Sezik E, Aslan M, Yesilada E, Ito S. Hypoglycemic activity of Gentianao livieriand isolation of the active constituent through bioassay-directed fractionation techniques. Life Sci 2005; 76(1):1223–1238.

  • Marles R, Farnsworth R. Hypoglycemic Activity of Methanolic Stem Bark of Adansonnia digitata Extract on Blood Glucose Levels of Streptozocin-Induced Diabetic Wistar Rats. Phytomedicine 1995; 2(1): 137–189.

  • Poongunran J, Perera HK, Fernando WIT, Jayasinghe L, Sivakanesan. α-Glucosidase and α-Amylase Inhibitory Activities of R. Nine Sri Lankan Antidiabetic Plants. British Journal of Pharmaceutical Research 2015; 7(5): 365-374.

  • Shareef MI, Reddy PJM, Gopinath SM, Dayananda KS, Mandal A. In vitro α-amylase inhibitory activity of the leaves of Tinospora cordifolia. International Journal of Innovative Research in Science, Engineering and Technology 2014; 3(3): 10091-10096.

  • Vennila V, Pavithra V. In-Vitro alpha amylase and alpha glucosidase Inhibitory Activity of Various Solvent Extracts of Hybanthus enneaspermus Linn. World Journal of Pharmacy and Pharmaceutical Sciences 2015; 4 (04): 1425-1437.

  • Singh NP, Kar Durga Madhab, Shweta P. Antidiabetic activity and modulation of Antioxidant status by fractions of Argemon mexicana in alloxan induced diabetic rats. International Journal of Green Pharmacy 2012; 6(1): 321-329.

  • Szkudelski T. The mechanism of Alloxan and Streptozotocin action in B cells of the rat pancreas. Physiol Res 2001; 50(5): 37-46.


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  • Identification of Phytochemical Constituents within the Rhizome Extract of Costusspeciosus (koen) using Gas Chromatography-Mass Spectrometry (GC-MS) Analysis and In vitro Anti Diabetic Activity

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Authors

S. Jebaseelan
Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai-20, India

Abstract


Costusspeciosus(koen) is widely used in traditional medicine for treatment of cancer and diabetic activity. Both its leaves were reported to possess antioxidant, antibacterial and anti-inflammatory activities. The rhizomes of Costusspeciosus(koen) were extracted with petroleum ether, chloroform, ethyl acetate and methanol by cold maceration extraction procedure and the resultant extract was subjected to phytochemical analyses using GC-MS technique. Three compounds with known biological activities were detected and identified based on GC-MS data as Costunolide. The findings of the study support the usage of Costusspeciosus(koen) in traditional medicinal as most of the identified compounds are known to possess biological activities. The various kinds of phytochemicals were detected and then in vitro antidiabetic activity of Costusspeciosus(koen) methanol extract was detected by using alpha amylase and alpha glucosidase enzyme in an in vitro model. The study reveals the presence of phytochemicals such as carbohydrate, tannin, flavonoids, phenols etc., Among the various extracts the methanol extract which contains the large number of phytoconstituents. The Costusspeciosus(koen) methanol extract has been successfully inhibited both the enzymes in an in vitro model. The methanol extract of Costusspeciosus(koen) inhibited the alpha amylase and alpha glucosidase enzymes as 65.34% and 58.48% respectively. From the result obtained in the current studies, the Costusspeciosus(koen) extract have a prominent antidiabetic property in an in vitro model.

Keywords


Costusspeciosus(koen), Methanol Extract, Phytochemical Constituents, GC-MS, Compound Identification, in vitro Anti Diabetic Activity.

References