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Identification of Phytochemical Constituents within the Rhizome Extract of Costusspeciosus (koen) using Gas Chromatography-Mass Spectrometry (GC-MS) Analysis and In vitro Anti Diabetic Activity


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1 Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai-20, India
     

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Costusspeciosus(koen) is widely used in traditional medicine for treatment of cancer and diabetic activity. Both its leaves were reported to possess antioxidant, antibacterial and anti-inflammatory activities. The rhizomes of Costusspeciosus(koen) were extracted with petroleum ether, chloroform, ethyl acetate and methanol by cold maceration extraction procedure and the resultant extract was subjected to phytochemical analyses using GC-MS technique. Three compounds with known biological activities were detected and identified based on GC-MS data as Costunolide. The findings of the study support the usage of Costusspeciosus(koen) in traditional medicinal as most of the identified compounds are known to possess biological activities. The various kinds of phytochemicals were detected and then in vitro antidiabetic activity of Costusspeciosus(koen) methanol extract was detected by using alpha amylase and alpha glucosidase enzyme in an in vitro model. The study reveals the presence of phytochemicals such as carbohydrate, tannin, flavonoids, phenols etc., Among the various extracts the methanol extract which contains the large number of phytoconstituents. The Costusspeciosus(koen) methanol extract has been successfully inhibited both the enzymes in an in vitro model. The methanol extract of Costusspeciosus(koen) inhibited the alpha amylase and alpha glucosidase enzymes as 65.34% and 58.48% respectively. From the result obtained in the current studies, the Costusspeciosus(koen) extract have a prominent antidiabetic property in an in vitro model.

Keywords

Costusspeciosus(koen), Methanol Extract, Phytochemical Constituents, GC-MS, Compound Identification, in vitro Anti Diabetic Activity.
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  • Identification of Phytochemical Constituents within the Rhizome Extract of Costusspeciosus (koen) using Gas Chromatography-Mass Spectrometry (GC-MS) Analysis and In vitro Anti Diabetic Activity

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Authors

S. Jebaseelan
Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai-20, India

Abstract


Costusspeciosus(koen) is widely used in traditional medicine for treatment of cancer and diabetic activity. Both its leaves were reported to possess antioxidant, antibacterial and anti-inflammatory activities. The rhizomes of Costusspeciosus(koen) were extracted with petroleum ether, chloroform, ethyl acetate and methanol by cold maceration extraction procedure and the resultant extract was subjected to phytochemical analyses using GC-MS technique. Three compounds with known biological activities were detected and identified based on GC-MS data as Costunolide. The findings of the study support the usage of Costusspeciosus(koen) in traditional medicinal as most of the identified compounds are known to possess biological activities. The various kinds of phytochemicals were detected and then in vitro antidiabetic activity of Costusspeciosus(koen) methanol extract was detected by using alpha amylase and alpha glucosidase enzyme in an in vitro model. The study reveals the presence of phytochemicals such as carbohydrate, tannin, flavonoids, phenols etc., Among the various extracts the methanol extract which contains the large number of phytoconstituents. The Costusspeciosus(koen) methanol extract has been successfully inhibited both the enzymes in an in vitro model. The methanol extract of Costusspeciosus(koen) inhibited the alpha amylase and alpha glucosidase enzymes as 65.34% and 58.48% respectively. From the result obtained in the current studies, the Costusspeciosus(koen) extract have a prominent antidiabetic property in an in vitro model.

Keywords


Costusspeciosus(koen), Methanol Extract, Phytochemical Constituents, GC-MS, Compound Identification, in vitro Anti Diabetic Activity.

References