Research Journal of Pharmacy and Technology

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A Comparative Study on the Biological Activities of Medicinal Plants Leaf Extracts Belongs to the Family Fabaceae


Affiliations
1 School of Bioscience and Technology, Vellore Institute of Technology, Vellore-632014, Tamil Nadu, India
     

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The present study aims to analyze the phytochemicals, antioxidant, antimicrobial and in vitro anti-inflammatory activity of three different plant leaf extracts such as Pithecellobium dulce, Sesbania grandiflora and Lablab purpureus belongs to the family fabaceae. Qualitative phytochemical screening of different plants leaf powder confirmed the presence of tannins, alkaloids, phenols, terpenoids, flavonoids and anthraquinone glycosides. Methanolic leaf extract of Sesbania grandiflora plant possess highest total phenolic content of about 14.21 ± 0.011mg of GAE/g of extract whereas, Petroleum ether leaf extract of Lablab purpureus plant exhibits highest total phenolic content of about 11.45 ± 0.002 mg of GAE/g of extract. In DPPH assay, methanol leaf crude extract demonstrated 86±1.77% antioxidant activity in Sesbania grandiflora at 150μg/ml concentration. Whereas petroleum ether leaf crude extract demonstrated 82±1.66% antioxidant activity in Lablab purpureus at 150μg/ml concentration and in reducing power assay, both the methanol and petroleum ether leaf crude extract demonstrated 85±1.73% and 88±1.92% antioxidant activity in Sesbania grandiflora at the same concentration 150μg/ml. In egg albumin denaturation assay, both the methanol and petroleum ether leaf crude extract exhibited 78±1.38% and 81±1.79% anti-inflammatory activity in Lablab purpureus at the same 300μg/ml concentration. When compared to all plants extracts in antibacterial activity methanol leaf extract of Sesbania grandiflora demonstrated clear zone of inhibition against test microorganisms against five studied pathogens. When compared to all plants extracts in antifungal activity methanol leaf extract of Sesbania grandiflora demonstrated clear zone of inhibition against test pathogen Aspergillus niger. Results of this study conclude that the three different medicinal plant leaf extracts contains significant potential for pharmaceutical applications.

Keywords

Medicinal Plants, Phytochemicals, Antioxidant Activity, Antibacterial Activity, Antifungal Activity, In vitro Anti-Inflammatory Activity.
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  • Saleh Hosseinzadeh et al. The application of medicinal plants in traditional and modern medicine : A Review of Thymus vulgaris. International Journal of Clinical Medicine.6; 2015: 635–642.

  • Bassam Abdul Rasool Hassan. Pharmaceutica medicinal plants (Importance and Uses). Pharmaceutica Analytica Acta.3(10); 2012:

  • Baisakhi Bandyopadhyay and Santra S.C. Morphological,biochemical and cytological investigations of different plant species under tribe phaseolae of indian fabaceae. Indian Journal of Agricultural Research.43(3); 2009:187–193.

  • Mahbubur Rahman A.H.M and Ismot Ara Parvin M. Study of medicinal uses on fabaceae family at rajasthani, bangladesh. Research in Plant Sciences.2(1); 2016: 6–8.

  • Marimuthu Govindarajan et al. Adulticidal activity of Pithecellobium dulce (roxb.)Benth and Delonix elata ( L.)Gamble (family :Fabaceae) against the malaria vector anopheles stephensi (liston) (diptera : culicidae). International Journal of Pure and Applied Zoology.3(3); 2015: 274–278.

  • Suman kumari. Evaluation of phytochemical analysis and antioxidant and antifungal activity of Pithecellobium dulce leaves extract.Asian Journal of Pharmaceutical and Clinical Research.10(1); 2017: 370–375.

  • Anil Bobade. Methanolic extraction and isolation of bioactive chemicals from Pithecellobium dulce leaves by columnchromatography and GC-MS studies. Research Journal of Chemical Sciences.7(1); 2017: 49–52.

  • Zarena A.S, Shubha Gopal and Vineeth R. Antioxidant , antibacterial and cytoprotective activity of agathi leaf protein. Journal of Analytical Methods in Chemistry. 2014:1–8.

  • Ghanshyam Panigrahi, Chhayakanta Panda and Arjun Patra. Extract of Sesbania grandiflora ameliorateshyperglycemia in high fat diet-streptozotocin induced experimental diabetes mellitus. Scientifica.2016:1–10.

  • More A.D and Jagtap S.S. Induction of morphological leaf mutations in Lablab purpureus ( L.) sweet through chemical and physical mutagens. International Journal of Current Microbiology and Applied Sciences.5(10); 2016: 592–597.

  • Soetan K. O. Comparative evaluation of phytochemicals in the raw and aqueous crude extracts from seeds of three Lablab purpureus varieties. African Journal of Plant Science.6(15); 2012: 410––415.

  • Bhuvaneswari S et al. A comparative study on antibacterial activity of Common weeds. International Journal of Pharma and Bio Sciences. 2(1); 2011: 677-683.

  • Yosie Andriani et al. Phytochemical analysis, antioxidant, antibacterial and cytotoxicity properties of keys and cores part of Pandanus tectorius fruits. Arabian Journal of Chemistry. 2015:

  • Ranjithadhevi V. Sundar et al. Bioactivity of Phoenix dactylifera seed and its phytochemical analysis. International Journal of Green Pharmacy. 11 (2); 2017:

  • Padmanabhan P and Jangle S N. Evaluation of in-vitro antiinflammatory activity of herbal preparation, a combination of four medicinal plants.International Journal of Basic and Applied Medical Sciences. 2(1); 2011: 109 -116.

  • Tahiya Hilal Ali Alabri et al. Comparative study of phytochemical screening, antioxidant and antimicrobial capacities of fresh and dry leaves crude plant extracts of Datura metel L. Journal of King Saud University- Science. 26; 2014: 237–243.

  • Menaga D, Rajakumar S and Ayyasamy P M. Free radical scavenging activity of methanolic extract of Pleurotus florida mushroom. International journal of pharmacy and pharmaceutical sciences.5(4); 2013: 601-606.

  • Arif Ullah H M et al. Evaluation of antinociceptive, in-vivo & invitro anti-inflammatory activity of ethanolic extract of Curcuma zedoaria rhizome. BMC Complementary and Alternative Medicine.2014:1-12.

  • Pankaj Goyal et al. In vitro evaluation of crude extracts of Catharanthus roseus for potential antibacterial activity. International Journal of Green Pharmacy.2008: 176-181.

  • Ambikapathy V, Gomathi S and Panneerselvam A. Effect of antifungal activity of some medicinal plants against Pythium debaryanum (Hesse). Asian Journal of Plant Science and Research. 1(3); 2011: 131-134.

  • Mahmoud Rafieian-kopaei. Medicinal plants and the human needs. Journal of HerbMed Pharmacology.1(1); 2012: 1–2.

  • Ahmad F, Anwar F and Hira S. Review on medicinal importance of fabaceae family pharmacologyonline.3; 2016: 151–156.

  • Mukesh Kumar and Kiran Nehra. Antimicrobial activity of crude extracts of Pithecellobium dulce bark against various human pathogenic microbes. World journal of pharmacy and pharmaceutical sciences.3(5); 2014:1244–1260.

  • Sarasu Packiyalakshmi P, Premalatha R and Saranya A. Invitro Antimicrobial Activity of Leaf extracts from Sesbania grandiflora. International Journal of Current Microbiology and Applied Sciences.5(4); 2016: 21–27.

  • Shyamala gowri S and Vasantha K. Antioxidant activity of Sesbania grandiflora (pink variety) L. Pers. International Journal of Engineering Science and Technology. 2(9); 2010: 4350-4356.

  • Mahmood Reza Moein, Soheila Moein and Saeid Ahmadizadeh. Radical Scavenging and Reducing Power of Salvia mirzayaniiSubfractions.Molecules.13; 2008: 2804-2813.

  • Perumal Siddhuraju et al. Antioxidant capacity and total phenolic content of aqueous acetone and ethanol extract of edible parts of Moringa oleifera and Sesbania grandiflora. International Journal of Nutrition and Food Engineering. 8(9); 2014:1090-1098.

  • Pradeepa S, Subramanian S and Kaviyarasan V. Evaluation of antimicrobial activity of Pithecellobium dulce pod pulp extract. Asian Journal of Pharmaceutical and Clinical Research.7; 2011:1– 6.

  • Vineela Chadalavada et al. Study of antimicrobial activity of Dolicus lablab leaf extract. American Journal of Pharmtech Research.5(3); 2015: 288–296.


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  • A Comparative Study on the Biological Activities of Medicinal Plants Leaf Extracts Belongs to the Family Fabaceae

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Authors

Saranya Shankar
School of Bioscience and Technology, Vellore Institute of Technology, Vellore-632014, Tamil Nadu, India
Mythili Sathiavelu
School of Bioscience and Technology, Vellore Institute of Technology, Vellore-632014, Tamil Nadu, India

Abstract


The present study aims to analyze the phytochemicals, antioxidant, antimicrobial and in vitro anti-inflammatory activity of three different plant leaf extracts such as Pithecellobium dulce, Sesbania grandiflora and Lablab purpureus belongs to the family fabaceae. Qualitative phytochemical screening of different plants leaf powder confirmed the presence of tannins, alkaloids, phenols, terpenoids, flavonoids and anthraquinone glycosides. Methanolic leaf extract of Sesbania grandiflora plant possess highest total phenolic content of about 14.21 ± 0.011mg of GAE/g of extract whereas, Petroleum ether leaf extract of Lablab purpureus plant exhibits highest total phenolic content of about 11.45 ± 0.002 mg of GAE/g of extract. In DPPH assay, methanol leaf crude extract demonstrated 86±1.77% antioxidant activity in Sesbania grandiflora at 150μg/ml concentration. Whereas petroleum ether leaf crude extract demonstrated 82±1.66% antioxidant activity in Lablab purpureus at 150μg/ml concentration and in reducing power assay, both the methanol and petroleum ether leaf crude extract demonstrated 85±1.73% and 88±1.92% antioxidant activity in Sesbania grandiflora at the same concentration 150μg/ml. In egg albumin denaturation assay, both the methanol and petroleum ether leaf crude extract exhibited 78±1.38% and 81±1.79% anti-inflammatory activity in Lablab purpureus at the same 300μg/ml concentration. When compared to all plants extracts in antibacterial activity methanol leaf extract of Sesbania grandiflora demonstrated clear zone of inhibition against test microorganisms against five studied pathogens. When compared to all plants extracts in antifungal activity methanol leaf extract of Sesbania grandiflora demonstrated clear zone of inhibition against test pathogen Aspergillus niger. Results of this study conclude that the three different medicinal plant leaf extracts contains significant potential for pharmaceutical applications.

Keywords


Medicinal Plants, Phytochemicals, Antioxidant Activity, Antibacterial Activity, Antifungal Activity, In vitro Anti-Inflammatory Activity.

References