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Isolation and Characterization of Microbial Diversity of Soil of Dhanbad Coal Mines using Molecular Approach


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1 School of Bioengineering and Biosciences, Lovely Professional University, Punjab-144411, India
     

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Soil is the richest ecosystem there is, as far as the diversity and populations of microorganisms is concerned. It is known that there could be over ten billion microbes per gram of soil, making soil the ideal ecosystem for mining different genes, ORFs etc. Microbial Diversity forms the lifeline of an ecosystem, interfering, regulating and participating in the biogeochemical activities therein. The present study was conducted to identify key extremophilic species residing in the coal mines of Dhanbad plateau in Bihar, India. Dhanbad plateau soil presents a unique niche, rich in coal and minerals. Soil from the coal mines was analysed through metagenomic as well as culture-dependent approach to enlist the micro-organisms native to the place. The culturable fraction lead to the identification of Klebsiella spp, Enterobacteriaceae, Serratia marcescens, and Providencia spp.

Keywords

Microbial Diversity, Molecular techniques, 16S rRNA genes, Dhanbad Plateau, Metagenomics.
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  • Isolation and Characterization of Microbial Diversity of Soil of Dhanbad Coal Mines using Molecular Approach

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Authors

Prince Anand
School of Bioengineering and Biosciences, Lovely Professional University, Punjab-144411, India
Reena Singh Chopra
School of Bioengineering and Biosciences, Lovely Professional University, Punjab-144411, India
Daljeet Singh Dhanjal
School of Bioengineering and Biosciences, Lovely Professional University, Punjab-144411, India
Chirag Chopra
School of Bioengineering and Biosciences, Lovely Professional University, Punjab-144411, India

Abstract


Soil is the richest ecosystem there is, as far as the diversity and populations of microorganisms is concerned. It is known that there could be over ten billion microbes per gram of soil, making soil the ideal ecosystem for mining different genes, ORFs etc. Microbial Diversity forms the lifeline of an ecosystem, interfering, regulating and participating in the biogeochemical activities therein. The present study was conducted to identify key extremophilic species residing in the coal mines of Dhanbad plateau in Bihar, India. Dhanbad plateau soil presents a unique niche, rich in coal and minerals. Soil from the coal mines was analysed through metagenomic as well as culture-dependent approach to enlist the micro-organisms native to the place. The culturable fraction lead to the identification of Klebsiella spp, Enterobacteriaceae, Serratia marcescens, and Providencia spp.

Keywords


Microbial Diversity, Molecular techniques, 16S rRNA genes, Dhanbad Plateau, Metagenomics.

References