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Cytomorphometric Study of Exfoliated Cells of Buccal Mucosa in Type II-Diabetic Patient


Affiliations
1 Department of Oral Pathology and Microbiology, JSS Dental College and Hospital A Constituent College of JSSAHER Mysore-570022, India
2 Department of Oral and Maxillofacial Surgery, JSS Dental College and Hospital A Constituent College of JSSAHER Mysore-570022, India
3 Department of Orthodontics JSS Dental College and Hospital A Constituent College of JSSAHER Mysore-570022, India
     

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India is the country with highest prevalence of diabetes mellitus in the world. Other condition like premalignant lesions and condition, oral exfoliative cytology may be more appropriate as the non-invasive techniques for early diagnosis. So, cytology technique may help to diagnose diabetes disease and help to prevent the complications. Aims: The present study is conducted to evaluate the cytological and cytomorphometric changes of exfoliated cells of buccal mucosa in type-II diabetic patients. Methods and Material: The diabetic group comprised of 30 diabetic patients, the age of 30-60 years suffering from diabetes mellitus at least from one year. Control group comprised of 15 healthy volunteers above the age of 30 years. Smears were taken from the buccal mucosa and stained with papanicolaou stain. The stained smears were measured for the cellular diameter (CD) and nuclear diameter (ND) along the longest axis of the cell using software digital camera with axiovision of version 2.0. Collected data were analyzed using ‘Unpaired student t test’. Results: The results showed the mean nuclear diameter of the exfoliated cells of diabetic group was 11.198μm and that of the control group was 9.494μm and the difference showed very highly significance (P<0.001). The mean cell diameter of the exfoliated cells of diabetic group showed 56.52μm and the control group showed 53.148μm and the difference between these group was significant (P<0.01). Conclusions: This study suggests that there were significant cellular and nuclear changes in the diabetic patients. The knowledge of which can be helpful for the cytopathologist in the early recognition of the cellular changes in the diabetic patients.

Keywords

Diabetes Mellitus, Cytomorphometry, Oral Mucosa, Buccal Mucosa, Type II.
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  • Alberti KG, Zimmet PZ. Definition , diagnosis and classification of Diabetes mellitus and its complications. Part 1: Diagnosis and classification of diabetes mellitus Report of a WHO consultation. Geneva: World Health Organisation; 1999. (Department of Noncommunicable Disease Surveillance).

  • Williams G, Pickup JC. Handbook of diabetes. 3rd ed. London: Blackwell Publishing; 2004. pp. 56–62.

  • Alberti S, Spadella CT, Francischone TRCG, Assis GF, Cestari TM, Taveira LAA. Exfoliative cytology of the oral mucosa in type II diabetic patients: morphology and cytomorphometry. J Oral Pathol Med 2003; 32: 538-43.

  • Maeda MYS, Loreto CD, Shirata NK, Shih LWS, Cavaliere MJ, Filho AL et al. Image analysis of Nuclear / Cytoplasmic Ratio in Cervical smears to discriminate three grades of Cervical Intraepithelial Neoplasia. Acta Cytologica 1997; 41: 744- 748.

  • Manisha S, Hema S, Sushruth N, Pramod K. Cytomorphometric analysis of gingival epithelium and buccal mucos cells in type2 diabetes mellitus patients. JOMFP 2017, 21(2):224-228.

  • Jajarm HH, Mohtasham N, Moshaverinia M, Rangiani A. Evaluation of oral mucosa epithelium in type II diabetic patients by an exfoliative cytology method. J Oral Sci. 2008;3:335–40.

  • Jones AC , Pink FE, Sandow PL, Stewart CM, Migliorati CA, Baughman RA et al. The cytobrush plus cell collector in oral cytology. Oral Surg Oral Med Oral Pathol 1994;77:101-4.

  • Dandona P, Thusu K, Cook S, Snyder B, Makowski J, Armstrong D. Oxidative damage to DNA in diabetes mellitus. Lancet 1996; 347:444–5.

  • Seppala B, Sorsa T, Ainamo J. Morphometric analysis of cellular and vascular changes in gingival connective tissue in long-term insulin-dependent diabetes. J Periodontol 1997; 68(12):1237–45.

  • Tsuji S, Kawai N, Tsujii M, Kawano S, Hori M. Review article: inflammation related promotion of gastrointestinal carcinogenesis-a perigenetic pathway. Ailment Pharmacol Ther 2003; 18:82–9.

  • Seril DN, Liao J, Yang GY, Yang CS. Oxidative stress and ulcerative colitis- associated carcinogenesis: studies in humans and animal models. Carcinogenesis 2003; 24(3):353–62.


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  • Cytomorphometric Study of Exfoliated Cells of Buccal Mucosa in Type II-Diabetic Patient

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Authors

Vidya G. Doddawad
Department of Oral Pathology and Microbiology, JSS Dental College and Hospital A Constituent College of JSSAHER Mysore-570022, India
S. Shivananda
Department of Oral and Maxillofacial Surgery, JSS Dental College and Hospital A Constituent College of JSSAHER Mysore-570022, India
Pradeep Subbaiah
Department of Orthodontics JSS Dental College and Hospital A Constituent College of JSSAHER Mysore-570022, India

Abstract


India is the country with highest prevalence of diabetes mellitus in the world. Other condition like premalignant lesions and condition, oral exfoliative cytology may be more appropriate as the non-invasive techniques for early diagnosis. So, cytology technique may help to diagnose diabetes disease and help to prevent the complications. Aims: The present study is conducted to evaluate the cytological and cytomorphometric changes of exfoliated cells of buccal mucosa in type-II diabetic patients. Methods and Material: The diabetic group comprised of 30 diabetic patients, the age of 30-60 years suffering from diabetes mellitus at least from one year. Control group comprised of 15 healthy volunteers above the age of 30 years. Smears were taken from the buccal mucosa and stained with papanicolaou stain. The stained smears were measured for the cellular diameter (CD) and nuclear diameter (ND) along the longest axis of the cell using software digital camera with axiovision of version 2.0. Collected data were analyzed using ‘Unpaired student t test’. Results: The results showed the mean nuclear diameter of the exfoliated cells of diabetic group was 11.198μm and that of the control group was 9.494μm and the difference showed very highly significance (P<0.001). The mean cell diameter of the exfoliated cells of diabetic group showed 56.52μm and the control group showed 53.148μm and the difference between these group was significant (P<0.01). Conclusions: This study suggests that there were significant cellular and nuclear changes in the diabetic patients. The knowledge of which can be helpful for the cytopathologist in the early recognition of the cellular changes in the diabetic patients.

Keywords


Diabetes Mellitus, Cytomorphometry, Oral Mucosa, Buccal Mucosa, Type II.

References