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Formulation and Characterization of Cedrus deodara Oil Emulsion and studies on its activity against representative food and plant pathogens


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1 School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India, 632014, India
     

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Food items and plants are mostly affected by bacterial pathogens that results in spreading of infection among consumers of contaminated foods and also yield loss in agricultural crops respectively. An essential oil such as cedarwood oil extracted from Cedrus deodara has excellent insecticidal, pesticidal and antimicrobial activities. In the present study, surfactants Tween 20 and Span 80 were used for emulsifying cedarwood oil to produce an oil in water emulsion that was found to be stable for the observed period of 120 days. The emulsion was characterized extensively by zeta potential, electrophoretic mobility, conductivity, turbidity, microscopic and FTIR studies with favorable results. The activity of the oil and its emulsion was checked against the food and plant pathogens and good activity was observed against all organisms.

Keywords

Cedrus deodara, Food pathogens, Tween 20, Span 80, Xanthomonas oryzae.
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  • Eller FJ and Taylor SL. Pressurized fluids for extraction of cedarwood oil from Juniperus Virginiana. Journal of Agricultural and Food Chemistry. 52 (8); 2004: 2335-2338.
  • Adams RP. Cedarwood oil - Analyses and properties. Essential oils and waxes. Springer, Berlin, Heidelberg. 1991; pp. 159-173.
  • Prabu seenivasan S, Jayakumar M and Ignacimuthu S. In the vitro antibacterial activity of some plant essential oils. Bio Med Central Complementary and Alternative Medicine. 6 (1); 2006: 39.
  • Hammer KA, Carson CF and Riley TV. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology. 86(6); 1999: 985-990.
  • Singh D and Agarwal SK. Himachalol and β-himachalene: Insecticidal principles of himalayan cedarwood oil. Journal of Chemical Ecology. 14 (4); 1988: 1145-1151.
  • Amer A and Heinz M. Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitology Research.99 (4); 2006: 466-472.
  • Johnston WH, Karchesy JJ, Constantine GH and Craig AM. Antimicrobial activity of some Pacific Northwest woods against anaerobic bacteria and yeast. Phytotherapy Research. 15 (7); 2001: 586-588.
  • Sabine JR. Exposure to an environment containing the aromatic red cedar, Juniperus Virginiana: pro carcinogenic, enzyme-inducing and insecticidal effects. Toxicology. 5 (2); 1975: 221-235.
  • Ramadass M and Thiagarajan P. Importance and Applications of Cedar oil. Research Journal of Pharmacy and Technology 8 (12); 2015: 1-5.
  • Daskalov H. The importance of Aeromonas hydrophila in food safety. Food Control. 17 (6); 2006: 474-483.
  • Tewari A andAbdullah S. Bacillus cereus food poisoning: International and Indian perspective. Journal of Food Science and Technology. 52 (5); 2015: 2500-2511.
  • Chiu CH, Su LH and Chu C. Salmonella enterica serotype Choleraesuis: epidemiology, pathogenesis, clinical disease, and treatment. Clinical Microbiology Reviews. 17; 2004: 311-322.
  • Farber JM and Peterkin PI. Listeria monocytogenes, a food-borne pathogen. Microbiological Reviews. 55 (3); 1991: 476-511.
  • Rabbani GH and Greenough III WB. Food as a vehicle of transmission of cholera. Journal of Diarrhoeal Diseases Research. 1999: 1-9.
  • Armstrong GL, Hollingsworth J and Morris Jr JG. Emerging foodborne pathogens: Escherichia coli O157: H7 as a model of entry of a new pathogen into the food supply of the developed world. Epidemiologic Reviews. 18 (1); 1996: 29-51.
  • Russell NJ and Gould GW, editors. Food preservatives. Springer Science & Business Media, 2003.
  • Davidson PM, Taylor TM and Schmidt SE. Chemical preservatives and natural antimicrobial compounds. Food Microbiology. American Society of Microbiology. 2013: 765-801.
  • Anand SP and Sati N. Artificial preservatives and their harmful effects: looking toward nature for safer alternatives. International Journal of Pharmaceutical Sciences and Research. 4 (7); 2013: 2496.
  • Lewus CB, Kaiser A and Montville TJ. Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bacteria isolated from meat. Applied and Environmental Microbiology. 57 (6); 1991: 1683-1688.
  • Turgis M, Vu KD, Dupont C and Lacroix M. Combined antimicrobial effect of essential oils and bacteriocins against foodborne pathogens and food spoilage bacteria. Food Research International. 48 (2); 2012: 696-702.
  • Moreira MR, Ponce AG, Del Valle CE and Roura SI. Inhibitory parameters of essential oils to reduce a foodborne pathogen. LWT-Food Science and Technology. 38 (5); 2005: 565-570.
  • Tripathi P and Dubey NK. Exploitation of natural products as an alternative strategy to control postharvest fungal rotting of fruit and vegetables. Postharvest Biology and Technology. 32 (3); 2004: 235-245.
  • Colborn T, VomSaal FS and Soto AM. Developmental effects of endocrine-disrupting chemicals in wildlife and humans. Environmental Health Perspectives. 101 (5); 1993: 378.
  • Van Hop D, Hoa PT, Quang ND, Ton PH, Ha TH, Van Hung N, Van NT, Van Hai T, Quy NT, Dao NT and Thom VT. Biological control of Xanthomonas oryzae pv. oryzae causing rice bacterial blight disease by Streptomyces toxytricini VN08-A-12, isolated from soil and leaf-litter samples in Vietnam. Biocontrol Science. 19 (3); 2014: 103-111.
  • Hastuti RD, Lestari Y, Suwanto A and Saraswati R. Endophytic Streptomyces spp. as biocontrol agents of rice bacterial leaf blight pathogen (Xanthomonas oryzae pv. oryzae). Hayati Journal of Biosciences. 19 (4); 2012: 155-162.
  • Velusamy P, Immanuel JE, Gnanamanickam SS and Thomashow L. Biological control of rice bacterial blight by plant-associated bacteria producing 2, 4-diacetyl phloroglucinol. Canadian Journal of Microbiology. 52 (1); 2006: 56-65.
  • Wiacek A and Chibowski E. Zeta Potential, effective diameter and multimodal size distribution in oil/water emulsion. Colloids and Surfaces A: Physiochemical and Engineering Aspects. 159 (2-3); 1999: 253-261.
  • Thanasukarn P, Pongsawatmanit R and McClements DJ. Influence of emulsifier type on freeze-thaw stability of hydrogenated palm oil-in-water emulsions. Food Hydrocolloids. 18; 2004: 1033-1043.
  • Banerjee K, Thiagarajan N and Thiagarajan P. Azadirachta indica A. Juss based emollient cream for potential dermatological applications. Indian Journal of Pharmaceutical Sciences. 78(3); 2016: 320-325.

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  • Formulation and Characterization of Cedrus deodara Oil Emulsion and studies on its activity against representative food and plant pathogens

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Authors

Manjula Ramadass
School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India, 632014, India
Syed Abdul Hakeem
School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India, 632014, India
Aravind Yaswanth Chandran
School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India, 632014, India
Gowtham Vadivelu
School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India, 632014, India
Padma Thiagarajan
School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India, 632014, India

Abstract


Food items and plants are mostly affected by bacterial pathogens that results in spreading of infection among consumers of contaminated foods and also yield loss in agricultural crops respectively. An essential oil such as cedarwood oil extracted from Cedrus deodara has excellent insecticidal, pesticidal and antimicrobial activities. In the present study, surfactants Tween 20 and Span 80 were used for emulsifying cedarwood oil to produce an oil in water emulsion that was found to be stable for the observed period of 120 days. The emulsion was characterized extensively by zeta potential, electrophoretic mobility, conductivity, turbidity, microscopic and FTIR studies with favorable results. The activity of the oil and its emulsion was checked against the food and plant pathogens and good activity was observed against all organisms.

Keywords


Cedrus deodara, Food pathogens, Tween 20, Span 80, Xanthomonas oryzae.

References