Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Characterization of Phosphate Solubilizing Microorganism Isolated from Soil


Affiliations
1 School of Studies in Life sciences, Pt. Ravishankar Shukla University, Raipur – 492010, Chhattisgarh, India
     

   Subscribe/Renew Journal


Phosphorus (P) is one of the most essential macro-elements required for growth and development of plants (including photosynthesis, energy and sugar production) . A greater part of P is present in the insoluble form and therefore, cannot be taken up by plants. The deficiency of P in turn severely restricts growth and yields in plants. Aspergillus species, a soil isolate had excellent potential to solubilize phosphate in vitro. In present study fungal strains isolated from soil having potential to solubilize phosphate were characterized. Fungal isolates were characterized as Aspergillus Sp. and Penicillium Sp. Both the isolates were identified by cultural and microscopic examination and are capable of phosphate solubilization.

Keywords

Aspergillus, Biofertilizer, Penicillium, Phosphate Solubilizing Fungi and Solubilization.
Subscription Login to verify subscription
User
Notifications
Font Size


  • V. Narsian, H.H. Patel, “Aspergillus aculeatus as rock phosphate solubilizers”, Soil Biol. Biochem. 32, pp. 559-565,2000.
  • Th eodorou ME, Plaxton WC. “Metabolic adaptations of plant respiration to nutritional phosphate deprivation”, Plant Physiol 101: 339-344, 1993.
  • M.S. Reddy, S. Kumar, B. Khosla, “Biosolubilization of poorly soluble rock phosphates by Aspergillus tubingensis and Aspergillus niger”, Bioresour. Technol. 84: pp. 187-189, 2002.
  • L. Khiari, L.E. Parent, “Phosphorus transformations in acid light textured soils treated with dry swine manure”. Can J Soil Sci 85:75-87, 2005.
  • A.K. Halder, A.K. Mishra, and P.K. Chakarbarthy, “Solubilization of inorganic phosphate by Bradyrhizobium”, Ind. J. Exp. Biol. 29: 28-31, 1991.
  • E. Nahas, “Factors determining rock phosphate solubilization by microorganisms isolated from soil” World J Microbiol Biotech 12: 567-572, 1996.
  • M.A. Whitelaw, “Growth promotion of plants inoculated with phosphate solubilizing fungi” Edited by Donald L. Sparks. Advances in Agronomy, Academic press 69 : 99-151, 2000.
  • R.I. Pikovskaya, “Mobilization of phosphorus in soil in connection with vital activity of some microbial species”, Microbiologiya, 17: 362-370, 1948.
  • M.B. Ellis, “Dematiceous hyphomycetes”, Commonwealth Mycological Institute, Kew, Surrey, England,1971.
  • H.L.Barnett, and B.B. Hunter, “Illustrated Genera of Imperfect Fungi”, 4th ed., St. Paul Minnesota, APS Press, 1998.
  • J.C. Gilman, “A manual of soil fungi”. Biotech books, Delhi, India. 2008.
  • J. Tarafdar, and A. “Jungk Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus”, J. Biol. Fert. Soils 3: 199-204,1987.
  • S.V. Mahamuni, P.V. Wani, and A.S. Patil, “Isolation of phosphate solubilizing fungi from rhizosphere of sugarcane and sugar beet using TCP and RP solubilization” Asian Journal of Biochemical and Pharmaceutical Research, 2: 237-244, 2012.
  • V. Deepa, A. Prasanna, B.P. Murthy, and R. Sridhar, “Efficient phosphate solubilization by fungal strains isolated from rice-rhizosphere soils for the phosphorus release”, Research Journal of Agriculture and Biological Sciences, 6: 487-492, 2010.
  • R. Saikia, K. Das, S. Deka, and P. Azad, “Status and Prospects of Soil Microbial Diversity of Dibru- Saikhowa Biosphere Reserve”, Himalayan Biosphere Reserves 6(1-2):61-63, 2004.
  • E.B.G. James, and K.D. Hyde, “Methods for the study of Mangrove Fungi, In: Mangrove Microbiology. Role of Microorganisms in Nutrient Cycling of Mangrove Soils and Waters”, Ed by A.D. Agate, C.V. Subramanian, H. Vannuccie. UNDP 9-27,1998.
  • P. Illmer, and F. Schinner, “Solubilization of inorganic phosphates by microorganisms isolated from forest soils”, Soil Biol Biochem.24: 89-395, 1992.
  • P. Vazquez, G. Holguin, M.E. Puente, A. Lopez Cortes, and Y. Bashan, “Phosphate solubilizing Microorganisms Associated with the Rhizosphere of Mangroves in a Semi-arid coastal Lagoon”, Biol. Fertil. Soils (30): 460-468, 2000.
  • S. Ravikumar, P. Williams, S. Shanthy, N. Anitha, A. Gracelin, S. Babu, and P.S. Parimala, “Effect of heavy metals (Hg and Zn) on the growth and phosphate solubilising activity in halophilic phosphobacteria isolated from Manakudi mangrove”, J. Environ. Biol., 28:109-114, 2007.
  • S. Fleischer, M. Bengtsson, and G. Johansson, “Mechanism of aerobic FE (III) phosphate solubilization at the sediment water interface” Verh. Int. Verein. Limnol., 23:1825-1829, 1988.
  • C. Nopparat, M. Jatupornpipat, and A. Rittiboon, “Isolation of phosphate solubilizing fungi in soil from Kanchanaburi”, Thailand. KMITL Sci. Tech. J. Vol. 7 No. S2 Nov. 2007.
  • S. M., Reddy, S. Kumar, K. Babita, and M.S. Reddy, “Bio solubilization of Poorly Soluble Rock Phosphates by Aspergillus tubingensis and Aspergillus niger”, Bioresource Technology, 84:187 – 189, 2002.
  • S.V. Mahamuni, P.V. Wani, and A.S. Patil, “Isolation of phosphate solubilizing fungi from rhizosphere of sugarcane and sugar TCP and RP solubilization”, Asian J. Biochem. Pharm. Res. 1:237-244, 2012.
  • J.M. Scervino, M.P. Mesa, I.D. Monica, M. Recchi, N.S. Moreno, A. Godeas, “Soil fungi isolates produced different organic acid patterns involved in phosphate salts solubilization”, Biol. Fertil. Soils 46:755- 763, 2010.
  • N. Gupta, J. Sabat, R Parida, D, Kerkatta, “Solubilization of tricalcium phosphate and rock phosphate by microbes isolated from chromite, iron and manganese mines”. Acta Bot. Croat. 66:197-204, 2007.

Abstract Views: 264

PDF Views: 0




  • Characterization of Phosphate Solubilizing Microorganism Isolated from Soil

Abstract Views: 264  |  PDF Views: 0

Authors

Pervez Ahmed Khan
School of Studies in Life sciences, Pt. Ravishankar Shukla University, Raipur – 492010, Chhattisgarh, India
Amia Ekka
School of Studies in Life sciences, Pt. Ravishankar Shukla University, Raipur – 492010, Chhattisgarh, India

Abstract


Phosphorus (P) is one of the most essential macro-elements required for growth and development of plants (including photosynthesis, energy and sugar production) . A greater part of P is present in the insoluble form and therefore, cannot be taken up by plants. The deficiency of P in turn severely restricts growth and yields in plants. Aspergillus species, a soil isolate had excellent potential to solubilize phosphate in vitro. In present study fungal strains isolated from soil having potential to solubilize phosphate were characterized. Fungal isolates were characterized as Aspergillus Sp. and Penicillium Sp. Both the isolates were identified by cultural and microscopic examination and are capable of phosphate solubilization.

Keywords


Aspergillus, Biofertilizer, Penicillium, Phosphate Solubilizing Fungi and Solubilization.

References