Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Multiple Shoot Regeneration in Seed-derived Immature Leaflet Explants of Red Dragon Fruit (Hylocereus costaricensis)


Affiliations
1 Department of Biotechnology, KLEF, Green Fields, Vaddeswaram, Guntur, India
2 Department of Biotechnology, Gandhi Institute of Technology and Management, Visakhapatnam, Andhra Pradesh, India
3 Prof. Jaya Shankar Telangana State Agricultural University, Hyderabad, Telangana, India
4 Rasi Seeds Pvt. Ltd. Tamilnadu, India
     

   Subscribe/Renew Journal


Dragon fruit which belongs to Cacteceae family was once widely grown as ornamental plant and is now considered as fruit crop. Though there were many reports on propagating dragon fruit inadequate information is available on obtaining best quality pant material. Hence the present work is carried out to find the effect of phyto harmones, 6-Benzylaminopurine (BAP) and Naphthalene acetic acid (NAA) on callus formation, shoot and ischolar_main regenerations from dragon fruit in vitro using stem cuttings as explants. Stem explants in Murashige Skoog (MS) medium containing 3mg/l BAP and 0.5mg/l NAA exhibited highest in vitro response for callus formation. Further, maximum number of shoots (13.2) was regenerated from explants when MS basal medium was supplemented with 3mg/l BAP and 0.5mg/l NAA. Within four weeks somatic shoots were raised from explants. It took 70 days for shoots to grow 2.0cm long. Subsequently regenerated shoots were transferred to ischolar_maining medium supplemented with 3mg/l BAP and 0.5mg/l NAA which proved to be effective concentrations for the growth and development of ischolar_mains (4.8) in vitro.

Keywords

Callus, Dragon Fruit, MS Basal Medium, NAA, BAP, Somatic Shoots.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Esquivel P., Stintzing F.C., Carle R. Comparison of morphological and chemical fruit traits from different pitaya genotypes (Hylocereus sp.) grown in Costa Rica. J. Appl. Bot. Food Qual. 2007a; 81: 7–14.

  • Le Bellec F. Pollinisation et fécondation d’ Hylocereus undatus et d’H. costaricensis à l’île de la Réunion. Fruits. 2004; 59: 411–422.

  • Rebecca O.P.S., Bovce A.N., Chandran S. Pigment identification and antioxidant properties of red dragon fruit (Hylocereus polyrhizus). Afr. J. Biotechnol. 2010; 9: 1450-1454.

  • Chang F.R., Yen C.R. Flowering and fruit growth in pitaya (Hylocereus undatus). J. Chinese Soc. Hort. Sci. 197; 43: 314- 321.

  • Choffe K.L., Victor J.M.R., Murch S.J., Saxena P.K. In vitro regeneration of Echinacea purpurea L.: Direct somatic embryogenesis and indirect shoot organogenesis in petiole culture. In Vitro Cell. Dev. Biol. 2000; 36: 30–36.

  • Dahanayake N., Ranawake A.L. Regeneration of dragon fruit (Hylocereus undatus) plantlets from leaf and stem explants. Trop. Agric. Res. Ext. 2011; 14: 85-89.

  • Lichtenzveig J., Abbo S., Nerd A., Tel-Zur N., Mizrahi Y. Cytology and mating systems in the climbing cacti Hylocereus and Selenicereus. American J. Bot. 2000; 87: 1058–1065.

  • Castillo R.M., Livera M.M., Alicia E., Brechú F., Márquez-Guzmán J. Compatibilidad sexual entre dos tipos de Hylocereus (Cactaceae). Rev. Bio. Trop. 2003; 51: 699–706.

  • Hartman H.T., Kester D.E., Davies Jr F.T., Geneve R.L. Plant Propagation: Principles and Practices. Prentice-Hall. London. 2002; 7: 886.

  • Thinesh A., Seran T.H. In vitro callogenesis from bud and stem explants of dragon fruit (Hylocereus undatus). Asian J Pharma Sci Tech. 2015; 5(4): 253-256.

  • Choffe K.L., Victor J.M., Murch S.J., Saxena P.K. In vitro regeneration of Echinacea purpurea L.: direct somatic embryogenesis and indirect shoot organogenesis in petiole culture. In Vitro Cell Develop Biol Plant. 2000; 36(1): 30-36.

  • Malathi R., Melchias G., Chandrasekar S., Cholarajan A. Role of Aluminium and Phosphorus on the Secretion and Behaviour of Acid Phosphatase in Callus Culture of Brassica juncea. Research J Engineering Tech. 2012; 3(4): 259-261.

  • Dahanayake N., Xiao-Lu Chen., Fu-Cheng Zhao., Yue-Sheng Yang., Hong Wu. An Efficient In Vitro Propagation System for Purple Cone-Flower (Echinacea Purpurea L). J of Tropical Agric Res and Extension. 2010; 13(2): 29-32.

  • Ahmad I., Hussan T., Ashraf I., Nafees M., Maryam, Rafay M, Iqbal M. Lethal effects of secondary metabolites. Am. Eurasian J. Agric. Environ. Sci. 2013; 13: 539-547.

  • Meenashree B., Kathiravan G., Srinivasan K., Rajangam B. Effect of Plant Hormones and Media Composition on Browning and Growth of Bacopa monnieri Callus Cultures. Research J Pharma Tech. 2017; 10(2): 497-500.

  • Dahanayake N., Ranawake A. L. Regeneration of Dragon fruit (Hylecereus undatus) plantlets from leaf and stem explants. Tropical Agric Res and Extension. 2011; 14(4): 85-89.

  • Sauve J.R., Mmbaga T.M., Zhou S. In vitro regeneration of the Tennessee coneflower (Echinacea tennesseensis). In Vitro Cell. Dev. Biol. Plant. 2004; 40: 325-327.

  • Rathnan Rahna K., Francis Jose., Irene Pius., Lamia Sathar., Nihil Jimmy., Shwetha Sasidharan., Ambili Mechoor. Comparative Study of Antimicrobial Activity of Leaves and Callus Extract of Ipomea turpethum against Antibiotic Resistance Pathogenic Microorganisms. Research J Pharma Tech. 2012; 5(6): 805-808

  • Yassen M.Y. Micropropagation of pitaya (Hylocereus undatus Britton & Rose) In Vitro Cell. Dev. Biol.Plant. 2002; 38: 427-429.

  • N Tel-Zur., S Abbo., D Bar-Zvi., Y Mizrahi ci. Clone Identification and genetic relationship among vine cacti from the genera Hylocereus and Selenicereus based on RAPD analysis. Scientia Hortic. 2004; 100: 279–289.


Abstract Views: 271

PDF Views: 0




  • Multiple Shoot Regeneration in Seed-derived Immature Leaflet Explants of Red Dragon Fruit (Hylocereus costaricensis)

Abstract Views: 271  |  PDF Views: 0

Authors

D. Pedda Kasim
Department of Biotechnology, KLEF, Green Fields, Vaddeswaram, Guntur, India
N. Sai Kishore
Department of Biotechnology, Gandhi Institute of Technology and Management, Visakhapatnam, Andhra Pradesh, India
P. Suneetha
Prof. Jaya Shankar Telangana State Agricultural University, Hyderabad, Telangana, India
K. Bramareswara Rao
Rasi Seeds Pvt. Ltd. Tamilnadu, India
M. Naresh Kumar
Department of Biotechnology, KLEF, Green Fields, Vaddeswaram, Guntur, India
M. S. R. Krishna
Department of Biotechnology, KLEF, Green Fields, Vaddeswaram, Guntur, India

Abstract


Dragon fruit which belongs to Cacteceae family was once widely grown as ornamental plant and is now considered as fruit crop. Though there were many reports on propagating dragon fruit inadequate information is available on obtaining best quality pant material. Hence the present work is carried out to find the effect of phyto harmones, 6-Benzylaminopurine (BAP) and Naphthalene acetic acid (NAA) on callus formation, shoot and ischolar_main regenerations from dragon fruit in vitro using stem cuttings as explants. Stem explants in Murashige Skoog (MS) medium containing 3mg/l BAP and 0.5mg/l NAA exhibited highest in vitro response for callus formation. Further, maximum number of shoots (13.2) was regenerated from explants when MS basal medium was supplemented with 3mg/l BAP and 0.5mg/l NAA. Within four weeks somatic shoots were raised from explants. It took 70 days for shoots to grow 2.0cm long. Subsequently regenerated shoots were transferred to ischolar_maining medium supplemented with 3mg/l BAP and 0.5mg/l NAA which proved to be effective concentrations for the growth and development of ischolar_mains (4.8) in vitro.

Keywords


Callus, Dragon Fruit, MS Basal Medium, NAA, BAP, Somatic Shoots.

References