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Vegetable Waste as an Alternate Plant Tissue Culture Media for Laboratory and Industry


Affiliations
1 Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation (Deemed to be University) Paiyanoor, Chennai, India
2 Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal – 637215 Tamil Nadu, India
3 Department of Biotechnology, Satyabhama Institute of Science and Technology, Jeppiaar Nagar, Chennai – 600 119, India
4 Department of Biotechnology, Vivekanandha College of Engineering For Women, Elayampalayam, Tiruchengode, India
     

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The clean production concept shows a good utilization potential for solid vegetable waste. It could achieve a reduction of investment and raw materials and can contribute to waste minimised plant production. Thus rendering cheap and environmentally safe alternative to commercial media. At present study was aimed at finding the potential of vegetable waste as a culture media. Composition of the media and the efficiency of the media to support plant growth were tested. Vegetable waste of Cabbage, Beetischolar_main and Onion were collected and dried in the sun light for 2 weeks. Dried vegetable waste was grinded into fine powder. These vegetable waste media (alternative plant tissue culture media) was prepared and coconut water was added for natural hormone (cytokinin). The alternative media pH was 5.6 to 5.7 before sterilization. The vegetable waste powder was analysed for Macronutrients which include potassium (268 mg/kg), sodium (520 mg/kg), calcium (1591 mg/kg), while the micronutrients were analysed copper (5.421 mg/kg), iron (1101 mg/kg) and zinc. The growth was comparable to that obtained on routine commercial media.

Keywords

Vegetable Waste, BCO, Plant Tissue Culture Media, Cost Effective.
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  • Vegetable Waste as an Alternate Plant Tissue Culture Media for Laboratory and Industry

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Authors

R. Subbaiya
Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation (Deemed to be University) Paiyanoor, Chennai, India
B. Aakash
Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation (Deemed to be University) Paiyanoor, Chennai, India
A. Shanmugaraja
Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal – 637215 Tamil Nadu, India
R. Devika
Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation (Deemed to be University) Paiyanoor, Chennai, India
S. Chozhavendhan
Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation (Deemed to be University) Paiyanoor, Chennai, India
S. Vinoth
Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation (Deemed to be University) Paiyanoor, Chennai, India
G. Karthiga Devi
Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation (Deemed to be University) Paiyanoor, Chennai, India
M. Masilamani Selvam
Department of Biotechnology, Satyabhama Institute of Science and Technology, Jeppiaar Nagar, Chennai – 600 119, India
S. Chozhavendhan
Department of Biotechnology, Vivekanandha College of Engineering For Women, Elayampalayam, Tiruchengode, India

Abstract


The clean production concept shows a good utilization potential for solid vegetable waste. It could achieve a reduction of investment and raw materials and can contribute to waste minimised plant production. Thus rendering cheap and environmentally safe alternative to commercial media. At present study was aimed at finding the potential of vegetable waste as a culture media. Composition of the media and the efficiency of the media to support plant growth were tested. Vegetable waste of Cabbage, Beetischolar_main and Onion were collected and dried in the sun light for 2 weeks. Dried vegetable waste was grinded into fine powder. These vegetable waste media (alternative plant tissue culture media) was prepared and coconut water was added for natural hormone (cytokinin). The alternative media pH was 5.6 to 5.7 before sterilization. The vegetable waste powder was analysed for Macronutrients which include potassium (268 mg/kg), sodium (520 mg/kg), calcium (1591 mg/kg), while the micronutrients were analysed copper (5.421 mg/kg), iron (1101 mg/kg) and zinc. The growth was comparable to that obtained on routine commercial media.

Keywords


Vegetable Waste, BCO, Plant Tissue Culture Media, Cost Effective.

References