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The Efficacy of Human Dental Pulp Stem Cells in regenerating Submandibular Gland Defects in Diabetic Wistar Rats (Rattus novergicus)


Affiliations
1 Universitas Airlangga, Surabaya, Indonesia
2 Orthodontics Department, Universitas Airlangga, Surabaya, Indonesia
3 Oral Medicine Department, Universitas Airlangga, Surabaya, Indonesia
4 Periodontics Department, Universitas Airlangga, Surabaya, Indonesia
5 Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, Indonesia
6 Virology and Immunology Laboratory, Microbiology Department, Universitas Airlangga, Surabaya, Indonesia
     

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Chronic hyperglicemia in Diabetes Mellitus caused microangiopathy in salivary gland. Human Dental Pulp Stem Cells (HDPSCs) suspected can regenerate the defect. The aim of this study was to analyze the efficacy of HDPSCs in stimulating angiogenesis, acinar cell numbers and Transforming Growth Factor-β (TGF-β) serum to regenerate submandibular gland defects in diabetic Wistar rats. Twenty-four male Wistar (250-350 g) rats 3-months-old were used. Rats were divided into 4 groups (n=6 each: a positive control group on Day 7 (DM) (C+7), a positive control group on Day 14 (DM) (C+14), a treatment group on Day 7 (DM+5.105 HDPSCs transplantation intraglandular) (T7) and a treatment group on Day 14 (DM+5.105 HDPSCs transplantation intraglandular) (T14). Wistar Rats were administered with 30 mg of Streptozotocin per kg of bodyweight to induce Diabetes Mellitus (DM). Histopathological examination with HE staining was performed to analyse neovascularization and acinar cell numbers. ELISA was performed to measure TGF-β serum. Statistical analysis used: A Tukey HSD or Bonferroni test after ANOVA or Kruskal Wallis test was performed (p<0.05) based on a Saphiro Wilk and Levene’s test (p>0.05). The highest acinar cell number was found in the T7 group [513.167±136.17] with no significant difference [p=0.136, p<0.05]. The highest capillaries were found in T14 [10.667±4.54] and TGF-β serum level [168.87±37.38] with significant difference [p=0.006; p<0.05] and [p=0.008, p<0.05]. HDPSCs can regenerate submandibular gland defects in Diabetic Wistar rats by stimulating angiogenesis, acinar cells number and TGF – β serum.

Keywords

Human Dental Pulp Stem Cells, Submandibular Gland Defect, Diabetes Mellitus, Acinar Cells, Angiogenesis, Transforming Growth Factor-β.
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  • The Efficacy of Human Dental Pulp Stem Cells in regenerating Submandibular Gland Defects in Diabetic Wistar Rats (Rattus novergicus)

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Authors

Septiana P. Suciadi
Universitas Airlangga, Surabaya, Indonesia
Alexander P. Nugraha
Orthodontics Department, Universitas Airlangga, Surabaya, Indonesia
Diah S. Ernawati
Oral Medicine Department, Universitas Airlangga, Surabaya, Indonesia
Nurina F. Ayuningtyas
Oral Medicine Department, Universitas Airlangga, Surabaya, Indonesia
Ida B. Narmada
Orthodontics Department, Universitas Airlangga, Surabaya, Indonesia
Chiquita Prahasanti
Periodontics Department, Universitas Airlangga, Surabaya, Indonesia
Aristika Dinaryanti
Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, Indonesia
Igo Syaiful Ihsan
Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, Indonesia
Eryk Hendrinto
Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, Indonesia
Helen Susilowati
Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, Indonesia
Fedik Abdul Rantam
Virology and Immunology Laboratory, Microbiology Department, Universitas Airlangga, Surabaya, Indonesia

Abstract


Chronic hyperglicemia in Diabetes Mellitus caused microangiopathy in salivary gland. Human Dental Pulp Stem Cells (HDPSCs) suspected can regenerate the defect. The aim of this study was to analyze the efficacy of HDPSCs in stimulating angiogenesis, acinar cell numbers and Transforming Growth Factor-β (TGF-β) serum to regenerate submandibular gland defects in diabetic Wistar rats. Twenty-four male Wistar (250-350 g) rats 3-months-old were used. Rats were divided into 4 groups (n=6 each: a positive control group on Day 7 (DM) (C+7), a positive control group on Day 14 (DM) (C+14), a treatment group on Day 7 (DM+5.105 HDPSCs transplantation intraglandular) (T7) and a treatment group on Day 14 (DM+5.105 HDPSCs transplantation intraglandular) (T14). Wistar Rats were administered with 30 mg of Streptozotocin per kg of bodyweight to induce Diabetes Mellitus (DM). Histopathological examination with HE staining was performed to analyse neovascularization and acinar cell numbers. ELISA was performed to measure TGF-β serum. Statistical analysis used: A Tukey HSD or Bonferroni test after ANOVA or Kruskal Wallis test was performed (p<0.05) based on a Saphiro Wilk and Levene’s test (p>0.05). The highest acinar cell number was found in the T7 group [513.167±136.17] with no significant difference [p=0.136, p<0.05]. The highest capillaries were found in T14 [10.667±4.54] and TGF-β serum level [168.87±37.38] with significant difference [p=0.006; p<0.05] and [p=0.008, p<0.05]. HDPSCs can regenerate submandibular gland defects in Diabetic Wistar rats by stimulating angiogenesis, acinar cells number and TGF – β serum.

Keywords


Human Dental Pulp Stem Cells, Submandibular Gland Defect, Diabetes Mellitus, Acinar Cells, Angiogenesis, Transforming Growth Factor-β.

References