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Evaluation of In vitro Anti-Diabetic and Anti-Oxidant activities and Preliminary Phytochemical Screening of Gel, Epidermis and Flower Extract of Aloe vera
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Aloe vera (Aloe barbadensis Miller) is a perennial succulent plant belonging to the Aloeaceae family and has been used for many centuries for its medicinal properties. Among the Aloe vera plant parts, gel is being widely studied for their biological properties. However, epidermis and flowers are neglected and are not probed for their importance. In this study, the methanolic extracts of Aloe vera gel, leaf and flower were evaluated for their anti-oxidant and anti-diabetic potential in vitro and other phytochemical constituents. We observed that the Aloe vera gel, leaf and flowers have shown significant anti-oxidant potential. When compared between leaf, gel and flower of Aloe vera, the leaf showed better antioxidant activity in ABTS, DPPH, H2O2 radical scavenging activities, Metal chelating activity and reducing power. However, the gel extract showed better anti-diabetic efficacy compared to the flower extract with respect to α-amylase, α-glucosidase, DPP-IV inhibition and anti-glycation activities. This study suggests that different parts of Aloe vera exhibit great potential for antioxidant and anti-diabetic activity and may be useful in new health-care food supplements or nutraceutical.
Keywords
?Aloe vera, Gel, Epidermis, Flower, Phenolic Compounds, Antioxidant, Antidiabetic.
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- Gupta VK, Sharma SK. Plants as natural antioxidants. Indian Journal of Natural Products and Resources. 2014;5(4): 326-334.
- Debnath T, Ghosh M, Lee YM, Nath NCD, Lee KG and Lim BO. Identification of phenolic constituents and antioxidant activity of Aloe barbadensis flower extracts. Food and Agricultural Immunology. 2017;29(1):27-38.
- Karpagam GN, Gayathri. R, Vishnupriya. V. Bioactivity analysis of lemongrass oil. Research Journal of Pharmacy and Technology.2016;9(7):903-906.
- Rajendran R, Saleem Basha N, Ruby S. Evaluation of in vitro Antioxidant Activity of Stem barks and Stem wood of Premna serratifolia Linn., (Verbenaceae). Research Journal of Pharmacognosy and Phytochemistry. 2009;1(1):11-14.
- Hullatti K, Telagari M. In-vitro α-amylase and α-glucosidase inhibitory activity of Adiantum caudatum Linn. and Celosia argentea Linn. extracts and fractions. Indian Journal of Pharmacology. 2015;47(4):425-429.
- Pattabiraman K, Muthukumaran P. Antidiabetic and Antioxidant Activity of Morinda tinctoria roxb Fruits Extract in Streptozotocin-Induced Diabetic Rats. Asian Journal of Pharmacy and Technology. 2011;1(2):34-39.
- Hamman JH. Composition and applications of Aloe vera leaf gel. Molecules. 2008;13(8):1599-616.
- Boudreau MD, Beland FA. An evaluation of the biological and toxicological properties of Aloe barbadensis (Miller), Aloe vera. Journal of environmental science and health. Part C, Environmental carcinogenesis and ecotoxicology reviews. 2006;24(1):103-154.
- Patel DS, Shah PB, Managoli NB. Evaluation of In-vitro Anti-oxidant and Free Radical Scavenging activities of Withania somnifera and Aloe vera. Asian Journal of Pharmacy and Technology. 2012;2(4):143-147.
- Cock IE. The genus aloe: Phytochemistry and therapeutic uses including treatments for gastrointestinal conditions and chronic inflammation. Progress in drug research. 2015; 70:179-235.
- Noor A, Gunasekaran S, Soosai Manickam A, Vijayalakshmi MA. Antidiabetic activity of Aloe vera and histology of organs in streptozotocin-induced diabetic rats. Current Science. 2008;94(8):1070-1076.
- Elias J, Rajesh M, Anish N, Jayan N. In Vitro Antioxidant Activity of the Methanolic Extract of Simaruba glauca DC. Asian Journal of Research in Chemistry. 2010;3(2):312-315.
- Soares L, Silva L, Pezzini B. Spectrophotometric determination of the total flavonoid content in Ocimum basilicum L. (Lamiaceae) leaves. Pharmacognosy Magazine. 2015;11(41):96-101.
- Raja C, Venkataraman K. Aloe vera phytochemicals inhibits dipeptidyl peptdiase IV (DPP-IV), an anti-diabetic target. International Journal of Pharma and Bio sciences. 2016;7(3):120-128.
- Sathyanarayanan S, Chandran R, Thankarajan S, Abrahamse H and Thangaraj P. Phytochemical composition, antioxidant and anti-bacterial activity of Syzygium calophyllifolium Walp. fruit. Journal of Food Science and Technology. 2018;55(1):341-350.
- Pavithra S, Banu N. Free Radical Scavenging Activity and Total Antioxidant Capacity of Tin Chlorophyllin from Morinda citrifolia L. Research Journal of Pharmacy and Technology. 2017;10(2):453-455.
- Nikhat F, Satyanarayana D, Evs S. Isolation, Charectrisation and Screening of Antioxidant Activity of the Roots of Syzygiumcuminii (L) Skeel. Asian Journal of Research in Chemistry. 2009;2(2):218-221.
- Menaka, Venkatasubramanian C. Free radical scavenging activity of Lyophilised Syzygium cumini (L) Skeels fruit pulp (Jamun). Research Journal of Pharmacy and Technology. 2017;10(4):986-990.
- Mukhopadhyay D, Dasgupta P, Sinha Roy D, Palchoudhuri S, Chatterjee I, Ali S and Dastidar SG. A Sensitive In vitro Spectrophotometric Hydrogen Peroxide Scavenging Assay using 1,10-Phenanthroline. Free Radicals and Antioxidants. 2016;6(1):124-132.
- Gülçin I, Elmastat M, Aboul-Enein HY. Determination of Antioxidant and Radical Scavenging Activity of Basil (Ocimum basilicum L. Family Lamiaceae) Assayed by. Phytotherapy Research. 2007;21(4):354–361.
- Kumar A, Chattopadhyay S. DNA damage protecting activity and antioxidant potential of pudina extract. Food Chemistry. 2007;100(4):1377-1384.
- Pawar PS, Patil JT, Bendre RS. Synthesis, Characterization and In-Vitro Antidiabetic Studies of Vanadium Complexes derived from N2O2 donor Ligands. Asian Journalof Research in Chemistry. 2018;11(1):8-14.
- Khamis M, Talib F, Rosli NS, Mohd KS, Dharmaraj S,Latif ZA,Utharkar MRS andSrenivasan S.In vitro α-amylase and α-glucosidase inhibition and increased glucose uptake of Morinda citrifolia fruit and scopoletin. Research Journal of Pharmacy and Technology. 2015;8(2):189-193.
- Kazeem MI, Akanji MA, Hafizur RM, Choudhary MI. Antiglycation, antioxidant and toxicological potential of polyphenol extracts of alligator pepper, ginger and nutmeg from Nigeria. Asian Pacific Journal of Tropical Biomedicine. 2012;2(9):727-732.
- Tapas A, Sakarkar D, Kakde R. Flavonoids as Nutraceuticals: A Review. Tropical Journal of Pharmaceutical Research. 2008;7(3):1089-1099.
- López A, De Tangil MS, Vega-Orellana O, Ramírez AS and Rico M. Phenolic constituents, antioxidant and preliminary antimycoplasmic activities of leaf skin and flowers of Aloe vera (L.) Burm. f. (syn. A. barbadensis Mill.) from the Canary Islands (Spain). Molecules. 2013;18(5):4942-4954.
- Kaithwas G, Singh P, Bhatia D. Evaluation of in vitro and in vivo antioxidant potential of polysaccharides from Aloe vera (Aloe barbadensis Miller) gel. Drug and chemical toxicology. 2014;37(2): 135–143.
- Shukla S, Mehta A, Bajpai VK, Shukla S. In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. Food and Chemical Toxicology. 2009;47(9):2338-43.
- Kim J-S, Kwon C-S, Son KH. Inhibition of Alpha-glucosidase and Amylase by Luteolin, a Flavonoid. Bioscience, Biotechnology and Biochemistry. 2000;64(11):2458-61.
- Yagi A, Hegazy S, Kabbash A, Wahab EA-E. Possible hypoglycemic effect of Aloe vera L. high molecular weight fractions on type 2 diabetic patients. Saudi Pharmaceutical Journal. 2009;17(3):209-215.
- Misawa E, Tanaka M, Nomaguchi K, Yamada M, Toida T, Takase M, Iwatsuki K and Kawada T. Administration of phytosterols isolated from Aloe vera gel reduce visceral fat mass and improve hyperglycemia in Zucker diabetic fatty (ZDF) rats. Obesity Research and Clinical Practice. 2008;2(4):239-245.
- Gupta A, Jacobson GA, Burgess JR, Jelinek HF, Nichols DS, Narkowicz CK and Al-Aubaidy HA. Citrus bioflavonoids dipeptidyl peptidase-4 inhibition compared with gliptin antidiabetic medications. Biochemical and Biophysical Research Communications. 2018;503(1):21-25.
- Nicolle E, Souard F, Faure P, Boumendjel A. Flavonoids as Promising Lead Compounds in Type 2 Diabetes Mellitus: Molecules of Interest and Structure-Activity Relationship. Current Medicinal Chemistry. 2011;18(17):2661-72.
- Kaewnarin K, Niamsup H, Shank L, Rakariyatham N. Antioxidant and antiglycation activities of some edible and medicinal plants. Chiang Mai Journal of Science. 2014;41(1):105-116.
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