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Comparative Evaluation of Baicalein from Oroxylum indicum by using Conventional and Non-Conventional Extraction Methodology


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1 Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad - 431001, Maharashtra, India
     

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Baicalein (5, 6, 7-trihydroxyflavone) is flavonoid isolated from Oroxylum indicum belonging to family Bignoniaceae. Baicalein was extracted from O. indicum leaves by conventional and non-conventional extraction methodologies viz. Maceration, Soxhlet assisted (SAE), Ultra-sonic assisted (UAE) and accelerated solvent extraction (ASE). Ethanol was optimized among the various organic solvents used for the maceration process. All the extracts were compared with respect to the percentage yield obtained and the concentration of the baicalein in the different extracts. Among all the extraction methodologies Accelerated solvent extraction method proved the best in terms of percentage yield and concentration of baicalein. This method showed a maximum percent yield about 8.21% of the total extracts and high concentration of about 1.481 ng of baicalein in the extracts respectively, using pre-optimized ethanol as an extraction solvent. The accelerated solvent extraction method was found to be more efficient for the extraction of baicalein from leaves of O. indicum.

Keywords

Baicalein, Maceration, Soxhlet Assisted Extraction, Ultrasonic Assisted Extraction, Accelerated Solvent Extraction, HPLC.
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  • Comparative Evaluation of Baicalein from Oroxylum indicum by using Conventional and Non-Conventional Extraction Methodology

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Authors

Sachin Bhusari
Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad - 431001, Maharashtra, India
Supriya Morey
Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad - 431001, Maharashtra, India
Kanchan Nikam
Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad - 431001, Maharashtra, India
Pravin Wakte
Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad - 431001, Maharashtra, India

Abstract


Baicalein (5, 6, 7-trihydroxyflavone) is flavonoid isolated from Oroxylum indicum belonging to family Bignoniaceae. Baicalein was extracted from O. indicum leaves by conventional and non-conventional extraction methodologies viz. Maceration, Soxhlet assisted (SAE), Ultra-sonic assisted (UAE) and accelerated solvent extraction (ASE). Ethanol was optimized among the various organic solvents used for the maceration process. All the extracts were compared with respect to the percentage yield obtained and the concentration of the baicalein in the different extracts. Among all the extraction methodologies Accelerated solvent extraction method proved the best in terms of percentage yield and concentration of baicalein. This method showed a maximum percent yield about 8.21% of the total extracts and high concentration of about 1.481 ng of baicalein in the extracts respectively, using pre-optimized ethanol as an extraction solvent. The accelerated solvent extraction method was found to be more efficient for the extraction of baicalein from leaves of O. indicum.

Keywords


Baicalein, Maceration, Soxhlet Assisted Extraction, Ultrasonic Assisted Extraction, Accelerated Solvent Extraction, HPLC.

References