Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Production and Optimization of Extra-Cellular Protease from Ganoderma sp.


Affiliations
1 Department of Microbiology, Ethiraj College for Women, Chennai - 600008, India
     

   Subscribe/Renew Journal


Protease is an enzyme that belongs to the enzyme group, hydrolases whose catalytic function is to hydrolyse peptide bonds of proteins. Proteolytic enzymes find a wide application in various fields which includes medical therapy, especially cancer therapy, industrial processes, textile industries, genetic engineering, etc. Ganoderma is a Basidiomycete. It is one of the most common and significant wood decaying or rotting fungi. Besides being wood rotting fungi it is a medicinal mushroom. The scope of this study is to screen and to optimize various parameters for the production of protease from Ganoderma sp. Four different fungal strains were screened for the production of protease enzyme. Ganoderma sp was found to be the best strain among the four tested fungal strains for the production of protease. The growth medium for Ganoderma sp was screened. The production of protease enzyme by Ganoderma sp was experimented in the Czapekdox agar. Few parameters (pH, different substrate concentration) were optimized for the production of protease by Ganoderma sp. in Czapekdox agar medium. Various parameters (pH, different substrate concentrations, different substrates, different carbon sources, different nitrogen sources, different minerals) were optimized for the production of protease enzyme by Ganoderma sp. in Czapekdox broth. The protease enzyme was produced in large amount within three days in Czapekdox broth which had all the optimized parameters and was concentrated.

Keywords

Ganoderma, Protease.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Abhijit Ray. (2012). Protease Enzyme- Potential Industrial Scope. Int. J. Tech. 2(1):01-05.

  • Anja I. Pekkarinen., Berne L. Jones and Marja Leena Niku Paavola.(2002).Purification and properties of an alkaline proteinase of Fusarium culmorum. Eur J Biochem. 269:798-807.

  • Barett, A. J., Rawlings, N.D. and Woessner, J.F.(1998) Handbook of proteloytic Enzymes. Academic, New York.13-24.

  • Bhaskara Rao, K.V Veena S, Pooja S, Shriya Y, Shivram R.(2017). Novel Actinomycetales bacterium-PV7 isolated from Kanyakumari Marine Sediments: A Prospective Source for Industrial and Pharmaceutical Enzyme Production. Research Journal of Pharmacy and Technology. 10(5): 1471-1476.

  • Bradford, M.M. (1976). A rapid and semsitive method for the qualification of protein utilizing the principle of protein dye binding. Anal Biochem.72:248-254.

  • Chandra shekharaiah, K. S, H P Shweta, Rajiv Bharadwaj, N G Raju, N. Ramachandra Swamy. (2018). Characterization of Protease inhibitors from the seeds of Senna alata. Asian J. Research Chem. 11(2):351-354.

  • Dhar S.C. and Sreenivasulu S. (1984.).Studies on the use of dehairing enzyme for its suitability in the preparation of improved animal feed. Leather Sci.31:261-267.

  • Indranil Chanda, Smriti Rekha Chanda, Sadhan Kr Dutta. (2009). Anti-inflammatory Activity of a Protease Extracted from the Fruit Stem Latex of the Plant Artocarpus heterophyllus Lam. Research J. Pharmacology and Pharmacodynamics. 1(2): 70-72.

  • Jenitta XJ, Priya SE, Gnanadoss JJ. (2015). Optimization of Culture Conditions and Inducers for Improved Protease Production by Penicillium griseofulvum LCJ231 under Submerged Fermentation. International Journal of Advanced Biotechnology and Research. 6(2):152-60.

  • Krishna Kondragunta. V, Karuppuraj. V, Perumal. K. (2016). Antioxidant activity and Folic acid content in indigenous isolates of Ganoderma lucidum. Asian Journal of Pharmaceutical Analysis. 6(4)213-215.

  • Kunamani Adinarayan, Poluri Ellaiah and Davuluri Siva Prasad.(2003). Purification and partial characterization of thermostable serine alkaline protrease from a newly isolated Bacillus subtilis PE – 11. AAPS Pharm Sci tech. 4(4) Article 56.

  • Mala B.Rao., Tanksale, A.M., Ghatge, M.S and Deshpande, V.V.(1998). Molecular And biotechnological aspects of microbial proteases. Microbiology and Molecular biology Reviews. 62:597-635.

  • Madhusudhanan, J, M. Indumathi, S. Ammu. (2013). A Novel System for Drug Delivery Using Nanoparticle. Asian J. Pharm. Tech. Vol. 3: Issue 4, Pg 137-141.

  • Olugbenga, Orole and Osuyi, Uyi and Gbadeyan, Femi. (2016). Activity of Ganoderma lucidum on Serum Marker Enzymes in Ochratoxin a treated albino rat models. Research Journal of Pharmacology and Pharmacodynamics. 8(4). 151-156.

  • Ramachandran, T and T. Karthik. (2004). Application of genetic engineering and enzymes in textiles. IE (1)Journal-TX. 84:32-36.

  • Santhi R.(2014). Extracellular protease production by solid state fermentation using Punica granatum peel waste. Indo American Journal of Pharmaceutical Research. 4(6):2706-12.

  • Shanmugapriya, E, V. Ravichandiran, M. Vijey Aanandhi. (2016). Molecular docking studies on naturally occurring selected flavones against protease enzyme of Dengue virus. 9(7): 929-932

  • Vinyas Mayasa, Vijay Kumar Rasal, Banappa S Unger, Kartikeyan Subbarayan.(2016). Tolerability assessment and anti cancer activity of the partially purified protease inhibitors from Soyabean (Glycine max) orally ministered to rats. Research J. Pharm. and Tech. 9(7):925-928.

  • K. Viswanathan, L. Jeyanthi Rebecca. (2018).Screening of Marine Actinomycetes for Fibrinolytic Enzymes. Research J. Pharm. and Tech. 11(10): 4365-4369.

  • Usama Beshay. (2003). Production of alkaline protease from Teredinobacter tumirae cells immobilized on Ca-alginate beads. African Journal of Biotechnology. 2(3):60-65.


Abstract Views: 178

PDF Views: 0




  • Production and Optimization of Extra-Cellular Protease from Ganoderma sp.

Abstract Views: 178  |  PDF Views: 0

Authors

C. V. Vidhya
Department of Microbiology, Ethiraj College for Women, Chennai - 600008, India

Abstract


Protease is an enzyme that belongs to the enzyme group, hydrolases whose catalytic function is to hydrolyse peptide bonds of proteins. Proteolytic enzymes find a wide application in various fields which includes medical therapy, especially cancer therapy, industrial processes, textile industries, genetic engineering, etc. Ganoderma is a Basidiomycete. It is one of the most common and significant wood decaying or rotting fungi. Besides being wood rotting fungi it is a medicinal mushroom. The scope of this study is to screen and to optimize various parameters for the production of protease from Ganoderma sp. Four different fungal strains were screened for the production of protease enzyme. Ganoderma sp was found to be the best strain among the four tested fungal strains for the production of protease. The growth medium for Ganoderma sp was screened. The production of protease enzyme by Ganoderma sp was experimented in the Czapekdox agar. Few parameters (pH, different substrate concentration) were optimized for the production of protease by Ganoderma sp. in Czapekdox agar medium. Various parameters (pH, different substrate concentrations, different substrates, different carbon sources, different nitrogen sources, different minerals) were optimized for the production of protease enzyme by Ganoderma sp. in Czapekdox broth. The protease enzyme was produced in large amount within three days in Czapekdox broth which had all the optimized parameters and was concentrated.

Keywords


Ganoderma, Protease.

References