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Isolation and Identification of Avian Influenza Virus Sub type H9N2 in Poultry from Karbala City, Iraq


Affiliations
1 Department of Pathology and Poultry Disease, College of Veterinary Medicine, University of Kerbala, Karbala, Iraq
2 Department of Pathology and Poultry Disease, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
     

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Avian Influenza (AI) disease is an infectious and highly contagious viral disease infected poultry and cause respiratory, digestive distress and sudden death combined with high mortality may arrived to (90%) in infected field. In the last years, poultry industry in Iraq suffered from high mortality arrived to (20-90%) in some farms or more than with severe respiratory distress and sudden death. In addition, grossly characterized by tracheitis, lung congestion, hemorrhagic Payer΄s patches and cecal tonsils that led to high economic losses in poultry industry. This study was designed to isolate and identificate the causative agent by using HA-HI test and RT-PCR assays. A total of 20 samples (lung, trachea, spleen and intestine) were collected from organized farms from Karbala city suspected with AIV infection. All samples were inoculated into the allantoic cavity at (9-11) days old embryonated eggs with cultivation for six days. After that, the allantoic fluid was collected and stored in deep freeze. The HA-HI test was conducted to identify AI virus by using specific antisera. Results revealed that six samples were infected with AIV with HA-HI test and all samples were confirmed by using molecular methods (RT-PCR) test. Nucleotide sequences of H9N2 subtypes of AIV was obtained from NCBI websites and alignment with other researchers concerned in this topic. In conclusion: our results were obtained that all six farms infected with H9N2 subtypes.


Keywords

AIV, RT-PCR, HA-HI Test.
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  • 1-Spackman, E.; Pedersen, J.C.; Mckinley, E. T. and Gelb, J. (2013). Optimal specimen collection and transport methods for the detection of avian influenza and Newcastle disease virus. BMC Vet. Res., 9, 35.

  • Alexander D, Capua I, 2009, Avian Influenza and Newcastle Disease, Milan: Springer-Verlag Italia, ISBN 978-88-470-0825-0

  • Adams MJ, King AMQ, Carstens EB. Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2013). Archives of Virology 2013;158: 2023–2030. DOI:10.1007/s00705- 013-1688-5.

  • Monne I, Fusaro A, Nelson MI, et al. Emergence of a highly pathogenic avian influenza virus from a low-pathogenic progenitor. Journal of Virology 2014; 88: 4375–4388. DOI:10.1128/jvi.03181-13.

  • Modrow, S., D. Falke, U. Truyen, and H. Sch¨atzl (2010). Molekulare Virologie (3. ed.). Spektrum Akademischer Verlag GmbH Heidelberg-Berlin.

  • Bouvier, N. M. and P. Palese (2008). The biology of influenza viruses. Vaccine 26 Suppl 4, D 49–53.

  • Tong S, Zhu X, Li Y, et al. New world bats harbor diverse influenza A viruses. PLoS Pathogens 2013; 9: e1003657. DOI:10.1371/ journal. ppat.1003657.

  • Olson SH, Parmley J, Soos C, et al. Sampling strategies and biodiversity of influenza A subtypes in wild birds. PLoS ONE 2014; 9: e90826. DOI:10.1371/journal. pone.0090826.

  • OIE (2012). Newcastle disease. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Chapter 2.3.14. http://www.oie.int/ international-standard-setting/terrestrial-manual/access-online.

  • Alexander, D.J., 2009. Ecology and epidemiology of Newcastle disease. In: Avian influenza and Newcastle disease, a field and laboratory manual. In: I. Capua and D.J. Alexander, (eds.), foreword by J. Domenech and B. Vallat, Springer-Verlag, Italia. Via Decembrio, 28, I- 20137 Milan, pp: 19-26.

  • Schat KA, Bingham J, Butler JM, et al. Role of position 627 of PB2 and the multibasic cleavage site of the hemagglutinin in the virulence of H5N1 avian influenza virus in chickens and ducks. PLoS ONE 2012; 7: e30960. DOI:10.1371/journal. pone.0030960.

  • Gohrbandt S, Veits J, Hundt J, et al. Amino acids adjacent to the haemagglutinin cleavage site are relevant for virulence of avian influenza viruses of subtype H5. Journal of General Virology 2011; 92: 51–59. DOI:10.1099/vir.0.023887-0.

  • Al-Nasrawi, H. A Al-H. (2002) study around human infection avian influenza virus, master theses. Veterinary college /Baghdad university.

  • World animal health information database (2013). Detailed country(ies) disease incidence. http://www.oie.int/wahis_2/public/wahid.php/Disease information/ status detail, accessed: 28 August, 2013.

  • Suarez, D. L. (2008). Influenza A Virus. In S. D. E. (Ed.), Avian Influenza (pp. 3-22). Ames, Iowa USA: Blackwell Publishing.


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  • Isolation and Identification of Avian Influenza Virus Sub type H9N2 in Poultry from Karbala City, Iraq

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Authors

Mohammed A. Hussein
Department of Pathology and Poultry Disease, College of Veterinary Medicine, University of Kerbala, Karbala, Iraq
Emad J. Khammas
Department of Pathology and Poultry Disease, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq

Abstract


Avian Influenza (AI) disease is an infectious and highly contagious viral disease infected poultry and cause respiratory, digestive distress and sudden death combined with high mortality may arrived to (90%) in infected field. In the last years, poultry industry in Iraq suffered from high mortality arrived to (20-90%) in some farms or more than with severe respiratory distress and sudden death. In addition, grossly characterized by tracheitis, lung congestion, hemorrhagic Payer΄s patches and cecal tonsils that led to high economic losses in poultry industry. This study was designed to isolate and identificate the causative agent by using HA-HI test and RT-PCR assays. A total of 20 samples (lung, trachea, spleen and intestine) were collected from organized farms from Karbala city suspected with AIV infection. All samples were inoculated into the allantoic cavity at (9-11) days old embryonated eggs with cultivation for six days. After that, the allantoic fluid was collected and stored in deep freeze. The HA-HI test was conducted to identify AI virus by using specific antisera. Results revealed that six samples were infected with AIV with HA-HI test and all samples were confirmed by using molecular methods (RT-PCR) test. Nucleotide sequences of H9N2 subtypes of AIV was obtained from NCBI websites and alignment with other researchers concerned in this topic. In conclusion: our results were obtained that all six farms infected with H9N2 subtypes.


Keywords


AIV, RT-PCR, HA-HI Test.

References