Research Journal of Pharmacy and Technology

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Phytochemical Analysis, Standardization and Cytotoxic Activity of Curcuma aureginosa Extract in Human Breast Cancer (MCF-7) Cell Line


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1 Department of Pharmacy, STIKES Mandala Waluya Kendari, South-East Sulawesi, Indonesia
     

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Last several years, researches about the plant as an alternative treatment of breast cancer have been developed. Curcuma aureginosa has been applied empirically as a breast cancer treatment and provide a satisfying result. The aim of this study is to examine cytotoxic activity of C.aureginosa extract to MCF-7 breast cancer cells, phytochemical analysis, and standardization of extract. Examination of breast cancer activity conducted by MTT method, extract was examined at concentration of 2000, 1000, 500, 250, 125, 62.5, 32.25, 15.625, and 7.8125 μg/ml, doxorubicin 1 μg/ml is applied as a positive control. Phytochemical Analysis conducted by GC-MS, and standardization of extract conducted based on Indonesia Pharmacopeia. The results reveal that extract has cytotoxic activity toward MCF-7 breast cancer cell. Concentration of 2000, 1000, 500, 250μg/ml has similar activity with doxorubicin 1 μg/ml. IC50 value of the extract is 76.24 μg/ml with 48 hours incubation. Standardization result determines extract profile and completes the standard of Indonesia Pharmacopeia. Phytochemical analysis result reveals Germacra-1(10), 4-diene-12-oic acid 6 alpha hydroxy gamma lactone (25.12%) and Cycloisolongifolene, 8, 9-dehydro-9-formyl- (21.61%) as a major compound. Cycloisolongifolene, 8, 9-dehydro-9-formyl-, Alpha-Humulene, Curcumene, and Curzerene assumed as cytotoxic toward MCF-7 breast cancer cell.

Keywords

Curcuma aureginosa, Breast Cancer, MCF-7, MTT, GC-MS, Standardization.
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  • Phytochemical Analysis, Standardization and Cytotoxic Activity of Curcuma aureginosa Extract in Human Breast Cancer (MCF-7) Cell Line

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Authors

Muhammad Isrul
Department of Pharmacy, STIKES Mandala Waluya Kendari, South-East Sulawesi, Indonesia
Risky Juliansyah
Department of Pharmacy, STIKES Mandala Waluya Kendari, South-East Sulawesi, Indonesia
Ahmad Saleh
Department of Pharmacy, STIKES Mandala Waluya Kendari, South-East Sulawesi, Indonesia
Wa Ode Yuliastri
Department of Pharmacy, STIKES Mandala Waluya Kendari, South-East Sulawesi, Indonesia
Jastria Pusmarani
Department of Pharmacy, STIKES Mandala Waluya Kendari, South-East Sulawesi, Indonesia
Himaniarwati
Department of Pharmacy, STIKES Mandala Waluya Kendari, South-East Sulawesi, Indonesia
Wa Ode Wahyuni Maulidina
Department of Pharmacy, STIKES Mandala Waluya Kendari, South-East Sulawesi, Indonesia

Abstract


Last several years, researches about the plant as an alternative treatment of breast cancer have been developed. Curcuma aureginosa has been applied empirically as a breast cancer treatment and provide a satisfying result. The aim of this study is to examine cytotoxic activity of C.aureginosa extract to MCF-7 breast cancer cells, phytochemical analysis, and standardization of extract. Examination of breast cancer activity conducted by MTT method, extract was examined at concentration of 2000, 1000, 500, 250, 125, 62.5, 32.25, 15.625, and 7.8125 μg/ml, doxorubicin 1 μg/ml is applied as a positive control. Phytochemical Analysis conducted by GC-MS, and standardization of extract conducted based on Indonesia Pharmacopeia. The results reveal that extract has cytotoxic activity toward MCF-7 breast cancer cell. Concentration of 2000, 1000, 500, 250μg/ml has similar activity with doxorubicin 1 μg/ml. IC50 value of the extract is 76.24 μg/ml with 48 hours incubation. Standardization result determines extract profile and completes the standard of Indonesia Pharmacopeia. Phytochemical analysis result reveals Germacra-1(10), 4-diene-12-oic acid 6 alpha hydroxy gamma lactone (25.12%) and Cycloisolongifolene, 8, 9-dehydro-9-formyl- (21.61%) as a major compound. Cycloisolongifolene, 8, 9-dehydro-9-formyl-, Alpha-Humulene, Curcumene, and Curzerene assumed as cytotoxic toward MCF-7 breast cancer cell.

Keywords


Curcuma aureginosa, Breast Cancer, MCF-7, MTT, GC-MS, Standardization.

References