Research Journal of Pharmacy and Technology

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A Preliminary Study on Phytochemical Screening, Proximate Analysis and Anti-Bacterial Activities of Andrographis paniculata Seed Extract


Affiliations
1 Department of Biochemistry, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Chennai, Tamil Nadu, India
     

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A preliminary investigation was done to screen the phytochemical contents and to find out the proximate composition and antibacterial activity of Andrographis paniculata seeds. Standard analytical procedures were followed and four different solvent extractions were done. Qualitative phytochemical screening of Andrographis paniculata seeds showed the presence of important phytochemical compounds in methanol seed extract viz., Flavonoids, Saponins, Tannins, Phenolic compounds, Terpenoids and Glycosides. Chloroform, ethyl acetate and methanol extracts reported the presence of tannins, Phenols and terpenoids. Flavonoids were present in chloroform and methanol extracts. The hexane extract did not show positive for any of the tests. Alkaloids was found to be absent in all the solvent extracts. Further, tannin concentration was found to be in high in methanol extract in comparison to other solvent extracts. Proximate composition recorded methanol extractive value of about 57.75% and was higher than water extractive value (46.23%). The moisture content of the crude drug was found to be 21.40% and foaming index was < 100%. Antibacterial activity was studied by assessing the minimum inhibitory concentration of methanol extract for bacterial strains. The MIC values of Salmonella typhi 59, Vibrio cholera and Shigellaboydii 8 was found to be 100 μg/ml and for Bacillus licheniformis and Staphylococcus aureus, MIC was found to be 10μg/ml. The preliminary investigation suggests that Andrographis paniculata seed extract have a rich profile of phytochemicals with significant proximate composition and good antibacterial activity. The findings can be taken as an initiative step for finding out the promising agents responsible for its phytochemical and antibacterial activities. This could further pave way to the research with respect to antioxidant studies of Andrographispaniculata seeds.

Keywords

Andrographis paniculata Seed Extract, Phytochemicals, Antibacterial, Antioxidant, MIC (Minimum Inhibitory Concentration).
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  • Shahid Akbar. Andrographis paniculata paniculata: A Review of Pharmacological activities and clinical effects. Alternative medicine review: A Journal of clinical therapeutic. 2011; 16(1): 66-77.

  • Archana P Raina, Kumar A, Pareek SK. Growth Inhibitor activity against several fungal and bacterial strains have been reported and found to possess antimalarial activity. Indian Journal of Pharmaceutical Research.2007;69(3):473-475.

  • Chao WW, Lin BF. Isolation and Identification of bioactive compounds in Androgarphis. Chinese Med. 2010; 5 (17): 1-15.

  • Edwin ES, Vasantha-Srinivasan P, Senthilnathan, Thanigaivel A, Ponsankar A, Pradeepa V, et al., Anti-dengue efficacy of bioactive andrographolide from Andrographispaniculata (Lamiales: Acanthaceae) against the primary dengue vector Aedesaegypti.

  • Soma Roy, Kiranmayee Rao, Bhuvaneswari C, Mangamoori Lakshmi Narasu, ArchanaGiri. Phytochemical analysis of Andrographis paniculata extract and its antimicrobial activity. World Journal of Microbiology and Biotechnology. 2009;26(1):85-91.

  • Samy RP, Thwin MM, Gopalakrishnakone P, Ignacimuthu S. Ethnobotanical survey of folk plants for the treatment of snakebites in Southern part of Tamilnadu, India. J Ethnopharmacol. 2008; 115: 302-312.

  • Kanokwan J and Nobuo N. Pharmacological aspects of Andrographispaniculata. Journal of Health Science .2008;54:370-381.

  • Radha R, Sermakkani M, Thangapandian V. Evaluation of phytochemical and antimicrobial activity of Andrographis paniculate Nees (Acanthaceae) aerial parts. International journal of Pharmacy and life sciences .2011;2(2):562-567.

  • Kokate CK. Practical Pharmacognosy. 2000; 218:50.

  • Silvia Siliani, Alissa Mattei, Luca Benevieri Innocenti, BrunoZanoni. Bitter taste and phenolic compounds in extra Virgin Olive oil: An empirical relationship. Journal of Food Quality. 2006;29(4):431-441.

  • Showket AD, Bashir AR, Abdul RW, Mushtaq AG. Resistance against Insect pests by plant phenolics and their derivative compounds. Chemical Science Review and Letters. 2017; 6(23):1941-1949.

  • Abubacker MN, Vasantha S. Antibacterial activity of ethanolic leaf extract of Andrographis paniculata Nees (Acanthaceae) and its bioactive compound Andrographolide. Drug Invention Today. 2009;2(10):440-442.

  • Basri DF, Saidi N, Mahari H, Saari S, Santhanam J. Preliminary screening for antimicrobial activity of the pulp of Canariumodontophyllum Miq. (Dabai) fruit. Global Journal of Pharmacology. 2014;8(2):213-220.

  • Vidita VB, Ajay KS, Kishor KD. Detection of Heavy metal contents and Proximate analysis of ischolar_mains of Anogeissuslatifolia. Journal of Pharmacognosy and Phytochemistry. 2013;1(6):61-65.

  • Renu S. Useful metabolites from plant tissue cultures. Biotechnology. 2005;4(2):79-93.

  • Yanishlieva NV, Marinova EM. Stabilisation of edible oils with natural antioxidants. European Journal of Lipid Science and Technology. 2001; 103:752-767.

  • Edreva A, Velikova V, Tsonev T, Dagnon S, Gürel AL, Aktas L. Stress-protective role of secondary metabolites: Diversity of functionsand mechanisms. Gen. Appl. Plant. Physiol. 2008; 34:67–78.

  • Ali Ghasemzadeh, Neda Ghasemzadeh. Flavonoids and phenolic acids: Role and biochemical activity in plants and human. Journal of medicinal plant research. 2011;5(31): 6697-6703.

  • Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010; 15:7313-7352.

  • Yu-Tang T, Hsiao-Ling C, Hsin-Chung T, Shang-Husn Y, Yi-Chun, Chuan-Mu C. Therapeutic potential of andrographolide isolated from the leaves of Andrographis paniculata Nees for treating Lung Adenocarcinomas. Evid Based Complementary and Alternative Medicine .2013. 305898.

  • Dudareva N, Pichersky E, Gershenzon J. Biochemistry of plant volatiles. Plant Physiology. 2004; 135:1893–1902.


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  • A Preliminary Study on Phytochemical Screening, Proximate Analysis and Anti-Bacterial Activities of Andrographis paniculata Seed Extract

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Authors

S. Geetha
Department of Biochemistry, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Chennai, Tamil Nadu, India
S. Rajeswari
Department of Biochemistry, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Chennai, Tamil Nadu, India

Abstract


A preliminary investigation was done to screen the phytochemical contents and to find out the proximate composition and antibacterial activity of Andrographis paniculata seeds. Standard analytical procedures were followed and four different solvent extractions were done. Qualitative phytochemical screening of Andrographis paniculata seeds showed the presence of important phytochemical compounds in methanol seed extract viz., Flavonoids, Saponins, Tannins, Phenolic compounds, Terpenoids and Glycosides. Chloroform, ethyl acetate and methanol extracts reported the presence of tannins, Phenols and terpenoids. Flavonoids were present in chloroform and methanol extracts. The hexane extract did not show positive for any of the tests. Alkaloids was found to be absent in all the solvent extracts. Further, tannin concentration was found to be in high in methanol extract in comparison to other solvent extracts. Proximate composition recorded methanol extractive value of about 57.75% and was higher than water extractive value (46.23%). The moisture content of the crude drug was found to be 21.40% and foaming index was < 100%. Antibacterial activity was studied by assessing the minimum inhibitory concentration of methanol extract for bacterial strains. The MIC values of Salmonella typhi 59, Vibrio cholera and Shigellaboydii 8 was found to be 100 μg/ml and for Bacillus licheniformis and Staphylococcus aureus, MIC was found to be 10μg/ml. The preliminary investigation suggests that Andrographis paniculata seed extract have a rich profile of phytochemicals with significant proximate composition and good antibacterial activity. The findings can be taken as an initiative step for finding out the promising agents responsible for its phytochemical and antibacterial activities. This could further pave way to the research with respect to antioxidant studies of Andrographispaniculata seeds.

Keywords


Andrographis paniculata Seed Extract, Phytochemicals, Antibacterial, Antioxidant, MIC (Minimum Inhibitory Concentration).

References