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Evaluation of Engineering Properties of Problematic Soils using Bacillus pasteurii


Affiliations
1 Department of Civil Engineering, Kongu Engineering College, Perundurai, Erode (dist.), T. N– 638060, India
2 Department of Civil Engineering, Bannari Amman Institute of Technology, Sathyamangalam, Erode (Dist.), T.N– 638401, India
3 Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode (Dist.), T.N-638401, India
4 Department of Civil Engineering, Kongu Engineering College, Perundurai, Erode (Dist.), T.N– 638 060, India
     

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Soil improvement method using enzymatic activity from bacteria Bacillus pasteurii that able to catalyse urea and calcium chloride enabling cementation solution has become an emerging approach to increase the shear strength of sand. The production of urease enzyme of B. pasteurii will be increased using specific medium. Determination of the volume of bacteria needed is based on the soil porosity is studied and analysed. The preliminary result of B. pasteurii that use specific medium could increase the urease enzyme activity and already verified using urease measurement kit. The treatment period required for the initiation of production of cementing material (calcite) has been reduced from one month to 3 days, while using this specific medium for enzyme urease production. This leads to the rapid filling of the soil pores that result in the hardening of soil. The bonding between the soil particles also increases that leads to increase in the density of soil. The final results of shear improvement are obtained by conducting Direct shear test and Unconfined compression test on sandy and silty soils respectively. Finally the cohesion between the soil particles was identified through the permeability test.

Keywords

B. pasteurii, Direct Shear Test, Permeability, Soil Pores, Unconfined Compression Test.
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  • Apichit Kampala and Suksun Horpibulsuk. Engineering Properties of Silty Clay Stabilized with Calcium Carbide Residue. Asce journal of materials in civil engineering. May 2013; 25(5): 632-644.

  • Achal V., Pan X. Characterization of Urease and Carbonic Anhydrase Producing Bacteria and Their Role in Calcite Precipitation. Springer Science + Business Media. LLC.2010.

  • Pham et.al,. Evaluating Strategies to Improve Process Efficiency of Denitrification -Based micp. Asce journal of geotechnical & geoenvironmental engineering. 2018; 144(8): 04018049. ISSN 1090-0241.

  • Soon et.al,. Factors Affecting Improvement in Engineering Properties of Residual Soil through Microbial-Induced Calcite Precipitation. Asce Journal Of Geotechnical & Geoenvironmental Engineering. March- 2014; 140(5): 04014006.

  • Sun et.al,. Improvement of Microbial-Induced Calcium Carbonate Precipitation Technology for Sand Solidification. ASCE Journal Of Materials In Civil Engineering. Aug-2018; 30(11): 04018301.

  • Wang et.al,. Surficial Soil Stabilization against Water-Induced Erosion Using Polymer-Modified Microbially Induced Carbonate Precipitation. Asce Journal Of Materials In Civil Engineering. 2018; 30(10): 04018267.

  • Ivanov V., Chu J. Applications of microorganisms to geotechnical engineering for bio-clogging and bio-cementation of soil in situ. Springer Science+Business Media B.V. 2008.

  • Guzmán M, Jean GD, Stephan G; Synthesis of silver nano-particles by chemical reduction method and their antibacterial activity. International Journal of Chemical and Biomolecular Engineering 2009; 2: pg-104-111.

  • S. Karthick Raja Namasivayam1, S. Nivash Kumar, Chandra Mohan. S, V. Gayathri. Biocompatible formulation of Probiotic bacteriaBacillus subtilis with agriculture based Products for the effective acid Tolerance Properties and Synergistic activity with Antibiotics. Research J. Pharm. and Tech. 2018; 11(1): 73-78

  • Salwa M. Al-Thawadi. High strength Instiu biocementation of soil by calcite precipitating locally isolated ureolytic bacteria.Ph.d. thesis book. 2008.

  • Soon et.al,. Improvements in engineering properties of soils through microbial-induced calcite precipitation. KSCE Journal of Civil Engineering. July- 2013; 17(4):718-728.

  • Thullner M., et al. Modeling of a microbial growth experiment with bioclogging in a two-dimensional saturated porous media flow field. 2003.

  • Tittelboom et al. Use of bacteria to repair cracks in concrete. Elsevier. 2009.

  • Angayarkanni T., Anitha Subash, Tamilselvi V., Kamalakannan A. Antimicrobial Compounds Production by Pseudomonas fluorescens and Bacillus subtilis. Asian J. Research Chem. January 2012;5(1): 61-64

  • Prerana H. Salodkar, Nikhil A. Maske, Dipali H. Chaudhari. Effect of Nylon Fiber, Fly Ash and Lime utilization for different Soil stabilization conditions: A Case Study. Research J. Science and Tech. Oct. – Dec. 2015;7(4):223-229.

  • Advait N. Bhange, Nikhil A. Maske, P. Salodkar. Utilization of Fly Ash, Lime and Synthetic Bag Fiber for Soil Stabilization. Research J. Engineering and Tech. 2014; 5(4): 195-203.

  • Govindasamy Vinodhini, et.al,. Assessment of Antimicrobial Property of a Secondary Metabolite Produced by an Enriched Bacterial Culture Isolated from Soil. Research J. Pharm. and Tech. Jan. 2015; 8(1): 51-53.

  • vanPaassen L.A. et al. Potential Soil Reinforcement by Biological Denitrification. http://dx.doi.org/10.15242/IICBE.C514577 25.2009.

  • Arya C F, et.al,. Microbial geotechnology: Evaluation of strength and structural properties of microbial stabilized mud block (MSMB). International Journal of Scientific & Engg Research. Jan.2016; Vol-7, issue-1. ISSN 2229-5518.

  • Deblina D, et.al,. Evaluation of Biosurfactant producing activity of Bacillus thuringiensis-VITNDUVNB04 isolated from oil contaminated soil by various methods. Research J. Pharm. and Tech 2016; 9(12):2186-2190.

  • K. Natarajan, et.al,.. A Study on Tri Calcium Phosphate Solubilisation Efficiency of Soil Microbes. Asian J. Research Chem. 3(4): Oct. - Dec. 2010; Page 1054-1057.

  • Abhijit Ray. Isolation and screening of antibiotic producing Actinomyces species from soil sample. Research J. Engineering and Tech. 3(3): July-Sept. 2012 page 199-201.


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  • Evaluation of Engineering Properties of Problematic Soils using Bacillus pasteurii

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Authors

P. Kulanthaivel
Department of Civil Engineering, Kongu Engineering College, Perundurai, Erode (dist.), T. N– 638060, India
B. Soundara
Department of Civil Engineering, Bannari Amman Institute of Technology, Sathyamangalam, Erode (Dist.), T.N– 638401, India
Arunava Das
Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode (Dist.), T.N-638401, India
V. S. Kayalvizhi
Department of Civil Engineering, Kongu Engineering College, Perundurai, Erode (Dist.), T.N– 638 060, India
J. Bindhu
Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode (Dist.), T.N-638401, India

Abstract


Soil improvement method using enzymatic activity from bacteria Bacillus pasteurii that able to catalyse urea and calcium chloride enabling cementation solution has become an emerging approach to increase the shear strength of sand. The production of urease enzyme of B. pasteurii will be increased using specific medium. Determination of the volume of bacteria needed is based on the soil porosity is studied and analysed. The preliminary result of B. pasteurii that use specific medium could increase the urease enzyme activity and already verified using urease measurement kit. The treatment period required for the initiation of production of cementing material (calcite) has been reduced from one month to 3 days, while using this specific medium for enzyme urease production. This leads to the rapid filling of the soil pores that result in the hardening of soil. The bonding between the soil particles also increases that leads to increase in the density of soil. The final results of shear improvement are obtained by conducting Direct shear test and Unconfined compression test on sandy and silty soils respectively. Finally the cohesion between the soil particles was identified through the permeability test.

Keywords


B. pasteurii, Direct Shear Test, Permeability, Soil Pores, Unconfined Compression Test.

References