Research Journal of Pharmacy and Technology

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Anti-Cancer Activity of Aloe Vera Ethanolic Leaves Extract against In vitro Cancer Cells


Affiliations
1 Department of Biochemistry, Shrimati Indira Gandhi College, Tiruchirappalli, India
2 Cluster for Integrative Physiology and Molecular Medicine (CIPMM), Universiti Kuala Lumpur Royal College of Medicine Perak, No.3, Jalan Greentown, 30450, Ipoh, Perak, Malaysia
3 Biochemistry Department, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai, India
     

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In the present study, anticancer and cytotoxicity of Aloe vera ethanolic leaves extract was evaluated as the leaves of A.vera are reported to have great medicinal value with potential therapeutic applications. The anticancer activities of the ethanolic leaves extract of A.vera were investigated using 3-(4,5-dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromide assay on three human cancer cell lines HepG2 (liver cancer), HeLa (human cervical carcinoma cell line) and A549 (human lung adenocarcinoma epithelial cell line).In anticancer studies, A.vera ethanolic leaves extract showed potent proliferation inhibitory activity against HepG2, HeLa and A549 cell lines. The results have shown that the A.vera ethanolic leaves extract contain some active ingredients with the potential of being anticancer agents. A.vera ethanolic leaves extract has the potential to fight against cancer cells. Further work should be carried out on the characterization of specific anticancer components of A.vera.

Keywords

Aloe vera, Cancer cell lines, Lipid peroxidation, MTT assay, Phytochemicals.
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  • Ochwang'i DO, Kimwele CN, Oduma JA, Gathumbi PK, Mbaria JM, Kiama SG. Medicinal plants used in treatment and management of cancer in Kakamega County, Kenya. J Ethnopharmacol. 2014;151(3):1040-1055.

  • Evans HS, Møller H, Robinson D, Lewis CM, Bell CMJ, Hodgson SV. The risk of subsequent primary cancers after colorectal cancer in southeast England. Gut. 2002;50(5):647-652.

  • Greenwell M, Rahman P. Medicinal Plants: Their Use in Anticancer Treatment. International journal of pharmaceutical sciences and research. 2015;6(10):4103-4112.

  • Cragg GM, Newman DJ. Medicinals for the millennia: the historical record. Ann N Y Acad Sci. 2001; 953:3-25.

  • Mehmood T, Maryam A, Ghramh HA, Khan M, Ma T. Deoxyelephantopin and Isodeoxyelephantopin as Potential Anticancer Agents with Effects on Multiple Signaling Pathways. Molecules. 2017;22(6).

  • Chandrasekar R, Sivagami B, Niranjan Babu M. A Pharmacoeconomic Focus on Medicinal Plants with Anticancer Activity. Research J. Pharmacognosy and Phytochemistry. 2018; 10(1): 91- 100.

  • Kadam Trupti V, Waghmare Sneha S, Darekar A B, Saudagar R B. An overview: Aloe vera. Res. J. Topical and Cosmetic Sci. 2014; 5(2): 62-67.

  • Akhilesh Gupta, Swati Rawat. Clinical importance of aloe vera: Review. Research J. Topical and Cosmetic Sci. 2017; 8(1):30-39.

  • Vogler BK, Ernst E. Aloe vera: a systematic review of its clinical effectiveness. Br J Gen Pract. 1999;49(447):823-828.

  • Kuchel PW. Schaum's Outline of Theory and Problems of Biochemistry: McGraw-Hill; 1998.

  • Pradeep Sahu, Kiran Sahu, Ravindra Dhar Dubey, Shilpi Chatterjee, Tanushree Chatterjee. Herbal Antioxidants: A Review. Research J. Pharmacognosy and Phytochemistry 2010; 2(6): 427-434.

  • Hu Y, Xu J, Hu Q. Evaluation of antioxidant potential of aloe vera (Aloe barbadensis miller) extracts. J Agric Food Chem. 2003;51(26):7788-7791.

  • Abhijit Ray. Scope of Aloe vera as Medicinal Plant and Skin Care. Res. J. Topical and Cosmetic Sci. 2012; 3(1): 1-3.

  • Elgorashi EE, van Staden J. Pharmacological screening of six Amaryllidaceae species. J Ethnopharmacol. 2004;90(1):27-32.

  • M S Saluja, B Sangameswaran, A Sharma, C Dubey. Phytochemical study and In-Vitro Cytotoxic Activity of Cinnamomum tammala Linn. Against Ehrlich Ascites Carcinoma (EAC) and Dalton’s Ascitic Lymphoma (DAL) Cell Lines. Research J. Pharmacognosy and Phytochemistry 2010; 2(1): 37-40.

  • Shalabi M, Khilo K, Zakaria MM, Elsebaei MG, Abdo W, Awadin W. Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells. Asian Pacific Journal of Tropical Biomedicine. 2015;5(5):375-3181.

  • Lim S-W, Loh H-S, Ting K-N, Bradshaw TD, Allaudin ZN. Reduction of MTT to Purple Formazan by Vitamin E Isomers in the Absence of Cells. Tropical Life Sciences Research. 2015;26(1):111-120.

  • Injac R, Strukelj B. Recent advances in protection against doxorubicin-induced toxicity. Technol Cancer Res Treat. 2008;7(6):497-516.

  • Husain S, Alam MA, Jahan N, Ahmed S, Kauser HS. Sibr (Aloe vera) and its therapeutic efficacy described in Unani Medicine: a review. Journal of scientific and Innovative Research. 2014; 3(5): 545-551.

  • Naveena, K. Bharath B, Selvasubramanian. Antitumor activity of Aloe vera against Ehrlich ascitis carcinoma (EAC) in Swiss albino mice. International Journal of Pharma and Bio Sciences. 2011; 2(2): 400-409.

  • Syed Shafeeq. R, Shekshavali. T, Syed Sulthan Ahamed. S N. A Review on Cassia auriculata. Res. J. Pharmacology and Pharmacodynamics.2018; 10(3): 141-145.

  • López Z, Knauth P, Avalos-Navarro G, Bocanegra-Garcia V, Ramírez de León J, Reynoso-Camacho R, et al. Cytotoxic Effects of Sábila (Aloe Vera): Commercial Products as Food Supplement. Journal of Chemical, Biological and Physical Sciences. 2014; 4(5):47-54.

  • SB Datir, AM Patel, AK Patel, PP Patil, FI Rohit, VD Thorat, DK Bharti, AB Ganjare. Evaluation of Antioxidant Activity of the Aerial Parts of the Abutilon indicum (Linn) Sweet (Malvaceae). Research J. Pharmacology and Pharmacodynamics. 2010; 2(5): 324-327.

  • Md. Omar Faruk, Dibyajyoti Saha, Srikanta Chowdhury, Swati Paul, Md. Golam Kabir. In vitro Antioxidant activity of Methanolic Aerial Part Extract of Mimosa pudica. Research J. Pharmacology and Pharmacodynamics.2012; 4(4): 202-205.

  • Ahmad R, Ahmad N, Naqvi AA, Shehzad A, Al-Ghamdi MS. Role of traditional Islamic and Arabic plants in cancer therapy. Journal of Traditional and Complementary Medicine. 2017;7(2):195-204.

  • Du Plessis LH, Hamman JH. In vitro evaluation of the cytotoxic and apoptogenic properties of aloe whole leaf and gel materials. Drug Chem Toxicol. 2014;37(2):169-177.

  • Salamah N, Ahda M, Bimantara S, Hanar R. Total phenolic content and in vitro evaluation of antioxidant activity of ethanol extract of Ganoderma amboinense. Natl J Physiol Pharm Pharmacol. 2018; 8(1): 97-101.

  • Sadika Banu, Ramakrishnaiah T. N. Screening of Garcinia cambogia for in-Vitro Anti-Cancerous Activity against Colon Adenocarcinoma (Caco-2) Cell Line. Res. J. Pharmacognosy and Phytochem. 2018; 10(4): 272-276.


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  • Anti-Cancer Activity of Aloe Vera Ethanolic Leaves Extract against In vitro Cancer Cells

Abstract Views: 289  |  PDF Views: 0

Authors

T. Karpagam
Department of Biochemistry, Shrimati Indira Gandhi College, Tiruchirappalli, India
Jannathul Firdous
Cluster for Integrative Physiology and Molecular Medicine (CIPMM), Universiti Kuala Lumpur Royal College of Medicine Perak, No.3, Jalan Greentown, 30450, Ipoh, Perak, Malaysia
Revathy
Department of Biochemistry, Shrimati Indira Gandhi College, Tiruchirappalli, India
Shanmuga Priya
Department of Biochemistry, Shrimati Indira Gandhi College, Tiruchirappalli, India
B. Varalakshmi
Department of Biochemistry, Shrimati Indira Gandhi College, Tiruchirappalli, India
S. Gomathi
Department of Biochemistry, Shrimati Indira Gandhi College, Tiruchirappalli, India
S. Geetha
Biochemistry Department, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai, India
Noorzaid Muhamad
Cluster for Integrative Physiology and Molecular Medicine (CIPMM), Universiti Kuala Lumpur Royal College of Medicine Perak, No.3, Jalan Greentown, 30450, Ipoh, Perak, Malaysia

Abstract


In the present study, anticancer and cytotoxicity of Aloe vera ethanolic leaves extract was evaluated as the leaves of A.vera are reported to have great medicinal value with potential therapeutic applications. The anticancer activities of the ethanolic leaves extract of A.vera were investigated using 3-(4,5-dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromide assay on three human cancer cell lines HepG2 (liver cancer), HeLa (human cervical carcinoma cell line) and A549 (human lung adenocarcinoma epithelial cell line).In anticancer studies, A.vera ethanolic leaves extract showed potent proliferation inhibitory activity against HepG2, HeLa and A549 cell lines. The results have shown that the A.vera ethanolic leaves extract contain some active ingredients with the potential of being anticancer agents. A.vera ethanolic leaves extract has the potential to fight against cancer cells. Further work should be carried out on the characterization of specific anticancer components of A.vera.

Keywords


Aloe vera, Cancer cell lines, Lipid peroxidation, MTT assay, Phytochemicals.

References