Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Studies on Antimicrobial Activity of a Metabolite Produced by Streptomyces malaysiensis Isolated from Termite Mound Soil


Affiliations
1 Pharmaceutical Biotechnology Department, Narasaraopet, Guntur, A.P-522601, India
2 A.U. College of Pharmaceutical Sciences, Visakhapatnam, India
     

   Subscribe/Renew Journal


To discover novel secondary metabolites, our approach is to investigate unexplored regions of the world with the aim of isolating bioactive actinomycetes from the termite mounds soil sample. Actinomycetes are soil microorganisms. They are a type of bacteria. They are the same as each microorganism and fungi, and have characteristics linking them to both groups Actinomycetes square measure typically believed to be the missing biological process link between microorganism and fungi, however they need more characteristics in common with microorganism than they are doing fungi.13 actinomycetes and 3 fungal isolates were screened from the soil sample and out of them 6 actinomycetes showed anti microbial activity by agar-overlay method, Cross streaking method and Cup plate method. By the Agar overlay method we can assure that the isolate TMS 1A is having anti bacterial activity and anti fungal activity by measuring the inhibition zone diameter as 40mm and 35mm respectively. Cross streaking method of the active compound obtained from isolate TMS 1A shows that it is having antimicrobial activity against Gram +ve, Gram -ve bacteria and fungi. Cup plate assay of the active compound obtained from isolate TMS 1A shows that it is having anti bacterial activity. Results from anti microbial activity revealed that our isolate TMS 1 08 09 2010 1A was found to have broad spectrum of anti microbial activity on both (Gram + ve and Gram – ve) bacteria and fungi. Further investigation is required to extract the Bioactive metalbolites from Streptomyces malaysiensis.

Keywords

Actinomycetes, Anti Microbial Activity, Microorganism, Termite, Isolate.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Anderson, AS; Wellington, Elizabeth (2001). "The taxonomy of Streptomyces and related genera". International Journal of Systematic and Evolutionary Microbiology 51 (3): 797–814. doi:10.1099/00207713-51-3-797.

  • Atta, 2010H.M. Atta Production, purification, physico-chemical characteristics and biological activities of antifungal antibiotic produced by Streptomyces species AZ-Z710 American-Eurasian J. Sci. Res., 5 (1) (2010) |

  • Atta et al., 2009H.M. Atta, A.T. Abul-Hamd, H.G. Radwan Production of Destomycin – a antibiotic by Streptomyces sp. using rice straw as fermented substrate. Commun. Appl. Biol. Sci., Ghent University, 74 (3) (2009),

  • Atta et al., 2011H.M. Atta, M.H. El-Sehrawi, A.S. Bahobail Antifungal macrodiode production by Streptomyces albidoflavus-143: fermentation purification and biological activities J. Am. Sci., 7 (3) (2011), pp. 13–22

  • Akagawa, H.; Okanishi, M.; Umezawa, H. (1975). "A Plasmid Involved in Chloramphenicol Production in Streptomyces venezuelae: Evidence from Genetic Mapping". Journal of General Microbiology 90 (2): 336–46. doi:10.1099/00221287-90-2-336. PMID 1194895.

  • Berdy, 1974 J. Berd : Recent developments of antibiotic research and classification of antibiotics according to chemical structure Adv. Appl. Microbiol., 14 (1974), pp. 309–406

  • Berdy, 1980a J. Berdy Recent advances in and prospects of antibiotics research Process Biochem., 15 (1980), pp. 28–35

  • Berdy, 1980 Berdy, J., 1980b. CRC Handbook of Antibiotic Compounds, first ed., vol. 1. CRC Press, Boca Raton (ISBN-13: 9780849334511).

  • Berdy, 1980c Berdy, J., 1980c. CRC Handbook of Antibiotic Compounds, first ed., vol. 2. CRC Press, Boca Raton (ISBN-13: 978-0849334528).

  • Bibb, 2005 M.J. Bibb Regulation of secondary metabolism in streptomycetes Curr. Opin. Microbiol., 8 (2005), pp. 208–215

  • Buchanan and Gibbons, 1974 R.E. Buchanan, N.E. Gibbons Bergey’s Manual of Determinative Bacteriology (eighth ed) The Williams & Wilkins Company, Baltimore (1974)

  • Bentley, S. D.; Chater, K. H.; Kieser, T.; Larke, L.; Murphy, L.; Oliver, K.; O'Neil, S.; Rabbinowitsch, E.; Rajandream, M.-A. et al. (2002). "Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)". Nature417 (6885): 141–7. Bibcode:2002Natur.417..141B. doi:10.1038/417141a.PMID 12000953.

  • Brawner, Mary; Poste, George; Rosenberg, Martin; Westpheling, Janet (1991). "Streptomyces: A host for heterologous gene expression". Current Opinion in Biotechnology 2 (5): 674–81. doi:10.1016/0958-1669(91)90033-2. PMID 1367716.


Abstract Views: 246

PDF Views: 0




  • Studies on Antimicrobial Activity of a Metabolite Produced by Streptomyces malaysiensis Isolated from Termite Mound Soil

Abstract Views: 246  |  PDF Views: 0

Authors

Uma Maheswari Kolipaka
Pharmaceutical Biotechnology Department, Narasaraopet, Guntur, A.P-522601, India
G. Girija Sankar
A.U. College of Pharmaceutical Sciences, Visakhapatnam, India

Abstract


To discover novel secondary metabolites, our approach is to investigate unexplored regions of the world with the aim of isolating bioactive actinomycetes from the termite mounds soil sample. Actinomycetes are soil microorganisms. They are a type of bacteria. They are the same as each microorganism and fungi, and have characteristics linking them to both groups Actinomycetes square measure typically believed to be the missing biological process link between microorganism and fungi, however they need more characteristics in common with microorganism than they are doing fungi.13 actinomycetes and 3 fungal isolates were screened from the soil sample and out of them 6 actinomycetes showed anti microbial activity by agar-overlay method, Cross streaking method and Cup plate method. By the Agar overlay method we can assure that the isolate TMS 1A is having anti bacterial activity and anti fungal activity by measuring the inhibition zone diameter as 40mm and 35mm respectively. Cross streaking method of the active compound obtained from isolate TMS 1A shows that it is having antimicrobial activity against Gram +ve, Gram -ve bacteria and fungi. Cup plate assay of the active compound obtained from isolate TMS 1A shows that it is having anti bacterial activity. Results from anti microbial activity revealed that our isolate TMS 1 08 09 2010 1A was found to have broad spectrum of anti microbial activity on both (Gram + ve and Gram – ve) bacteria and fungi. Further investigation is required to extract the Bioactive metalbolites from Streptomyces malaysiensis.

Keywords


Actinomycetes, Anti Microbial Activity, Microorganism, Termite, Isolate.

References