Research Journal of Pharmacy and Technology

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Investigation of Antioxidant and Cytotoxic Activities of Kaempferia galanga L.


Affiliations
1 Department of Pharmacy, BRAC University, Dhaka, Bangladesh
2 Department of Pharmacy, Southeast University, Dhaka, Bangladesh
     

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This research was performed to determine the antioxidant, total phenolic content and cytotoxicity activities of methanol and acetone extracts of Kaempferia galanga Linn (Zingiberaceae) leaves. In this particular study, In vitro antioxidant activity of the extracts of Kaempferia galanga L. leaves was determined by using DPPH (2, 2-diphenyl-1-picrylhydraziyl) free radical scavenging assay. Here, both Standards butyl-1-hydroxyl toluene (BHT) and ascorbic acid (ASA) showed IC50 value of 28.30 μg/mL and 5.90 μg/mL. Compared to the standard crude methanol extract (CME) and crude acetone extract (CAE) showed IC50 value of 611.82 μg/mL and 702.79 μg/mL. In total phenolic content test, CME showed 1.012 mg of gallic acid equivalents/gm and CAE showed -0.066 mg of gallic acid equivalents/gm. Moreover, brine shrimp lethality bioassay was performed to determine the cytotoxic effect and the LC50 value of the CME and CAE were 4.78 μg/mL and 0.11 μg/mL. Whereas, LC50 value of the vincristine sulphate was 0.45 μg/mL. In conclusion, this research confirmed that Kaempferia galanga L. leaves comprised with antioxidant and cytotoxicity activity which can be used for various medicinal purposes.

Keywords

Kaempferia galanga L., Total Phenol Content, Antioxidant, DPPH Free Radical Scavenging Assay, Cytotoxicity.
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  • Sass C, Iles WJ, Barrett CF, Smith SY, Specht CD. Revisiting the Zingiberales: using multiplexed exon capture to resolve ancient and recent phylogenetic splits in a charismatic plant lineage. Peer J, 2016; 4: e1584, PMID 26819846.

  • Voogelbreinder S. Garden of Eden: The Shamanic Use of Psychoactive Flora and Fauna, and the Study of Consciousness. 2009.

  • Ratsch C. The Encyclopedia of Psychoactive Plants: Ethnopharmacology and its Applications. Rochester: Park Street Press. 1998.

  • Hofmann A, Ratsch C and Schultes R. Plants of the Gods: Their Sacred, Healing, and Hallucinogenic Powers. Rochester: Healing Arts Press. 1992.

  • Kirtikar KR and Basu BD. Indian Medicinal Plants, volume IV. In: Blatter E, Caius JF, Mahaskar KS (eds.), Lalit Mohan Basu, Allahabad, India.1996.

  • Perry M, Metzger J. Medical Plants of East and Southeast Asia. Attributed Properties and Uses. Cambridge MIT Press, 1980; p. 442.

  • Kaushita B, Lekhya Priya C, Bhaskara Rao KV. HPLC analysis and antioxidant activities of hydroethanolic leaf extract of Kaempferia galanga Linn. International Journal of Pharm Tech Research 2015; 7(2):422-431.

  • Sirirugsa P. Thai Zingiberaceae: Species diversity and their uses. Pure &Appl Chemistry, 1997; 70(11): 4-5.

  • Spearing JK and Mahanty HK. The relationship of the African species of Kaempferia to those found in Asia.Abs.Xth International Botanical Congress, Edinburgh. 1964.

  • Umar MI, Asmawi MZB, Sadikun A, Altaf R, Iqbal MA. Phytochemistry and medicinal properties of Kaempferia galanga L. (Zingiberaceae) extracts. Afr. J. Pharm. Pharmacol, 2011; 5(14): 1638-1647.

  • Ahn YJ, Kim NJ, Byun SG, Cho JE, Chung K. Larvicidal activity of Kaempferia galanga rhizome phenylpropanoids towards three mosquito species. Pest Management Science 2008; 64(8):857–862.

  • Choochote W, Kanjanapothi D, Panthanga A, Taesotikul T, Jitpakdi A, Chaithong U. Larvicidal and repellent effects of Kaempferia galanga. South east Asian J Trap Med Public Health 1999; 30:470-476.

  • Umar MI, Asmawi, MZ, Sadikun A, Atangwho IJ, Yam MF, Altaf R, Ahmed A. Bioactivity-Guided Isolation of Ethyl-p-methoxycinnamate, an Anti-inflammatory Constituent, from Kaempferia galanga. Extracts Molecules, 2012; 17: 8720-8734.

  • Ridtitid W, Sae-wong C, Reanmongkol W, Wongnawa M. Antinociceptive activity of the methanolic extract of Kaempferia galanga Linn. In experimental animals.Journal of Ethnopharmacology, 2008; 118(2): 225-230.

  • Ahmed A, Husain A, Mujeeb M, Khan SA, Aldrami HAB, Bhandari A. Quantification of total phenol, flavonoid content and pharmacognostical evaluation including HPTLC fingerprinting for the standardization of Piper nigrum Linn fruits. Asian Pacific Journal of Tropical Biomedicine, 2015; 5(2): 101-107.

  • Olowa LF and Nuneza OM. Brine Shrimp Lethality Assay of the Ethanolic Extracts of Three Selected Species of Medicinal Plants from Iligan City, Philippines.2013.

  • Omar MN, Rahman SMMA, Ichwan SJA, Hasali NHM, Rasid FA, Halim FA. Cytotoxicity Effects of Extracts and Essential Oil of Kaempferia galanga on Cervical Cancer C33A Cell Line. Oriental Journal of Chemistry, 2017; 33(04), 1659–1664.


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  • Investigation of Antioxidant and Cytotoxic Activities of Kaempferia galanga L.

Abstract Views: 241  |  PDF Views: 0

Authors

Imon Rahman
Department of Pharmacy, BRAC University, Dhaka, Bangladesh
Md. Tanvir Kabir
Department of Pharmacy, BRAC University, Dhaka, Bangladesh
Md. Nasiful Islam
Department of Pharmacy, BRAC University, Dhaka, Bangladesh
Mushfiqa Muqaddim
Department of Pharmacy, BRAC University, Dhaka, Bangladesh
Shahana Sharmin
Department of Pharmacy, BRAC University, Dhaka, Bangladesh
Mohammed Sami Ullah
Department of Pharmacy, BRAC University, Dhaka, Bangladesh
Md. Sahab Uddin
Department of Pharmacy, Southeast University, Dhaka, Bangladesh

Abstract


This research was performed to determine the antioxidant, total phenolic content and cytotoxicity activities of methanol and acetone extracts of Kaempferia galanga Linn (Zingiberaceae) leaves. In this particular study, In vitro antioxidant activity of the extracts of Kaempferia galanga L. leaves was determined by using DPPH (2, 2-diphenyl-1-picrylhydraziyl) free radical scavenging assay. Here, both Standards butyl-1-hydroxyl toluene (BHT) and ascorbic acid (ASA) showed IC50 value of 28.30 μg/mL and 5.90 μg/mL. Compared to the standard crude methanol extract (CME) and crude acetone extract (CAE) showed IC50 value of 611.82 μg/mL and 702.79 μg/mL. In total phenolic content test, CME showed 1.012 mg of gallic acid equivalents/gm and CAE showed -0.066 mg of gallic acid equivalents/gm. Moreover, brine shrimp lethality bioassay was performed to determine the cytotoxic effect and the LC50 value of the CME and CAE were 4.78 μg/mL and 0.11 μg/mL. Whereas, LC50 value of the vincristine sulphate was 0.45 μg/mL. In conclusion, this research confirmed that Kaempferia galanga L. leaves comprised with antioxidant and cytotoxicity activity which can be used for various medicinal purposes.

Keywords


Kaempferia galanga L., Total Phenol Content, Antioxidant, DPPH Free Radical Scavenging Assay, Cytotoxicity.

References