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In-vitro Cell Line Models and Assay Methods to Study the Anti-Diabetic Activity
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Cases of Diabetes mellitus around the world are increasing day by day. It has been predicted that India, China and United States will be having the largest number of diabetic patients by 2030. Therefore, the anti-diabetic study is attracted a huge number of scientists and various in-vitro and in-vivo models has been developed for anti-diabetic study. In drug development, the in-vitro techniques are an important method of confirming a particular biological activity of a compound before animal experiments. In vitro cell lines are alternative to animal models to study the particular activity of drugs. To produce more reproducible results, the in-vitro cell lines from clonal cells, cell lines are used. Characteristics of cell lines are constantly changing because of continuous growth of cells without any limits being its disadvantage. This review attempts to bring together all reported in-vitro cell line models to study the anti-diabetic activity. These cell lines may prove essential for the development of new drugs for the treatment of diabetes mellitus. Study of anti-diabetics activity of various agents in cell line models is important for improving the knowledge and in providing a clear understanding of pathogenesis leading to the discovery of new therapies for the disease. Many cell lines have been discovered in past few decades for the in-vitro studies involving diabetes mellitus. The generation of cell lines may bedecreased the number of animals used for in-vivo studies. Using cell lines for anti-diabetic studies also put aside the painful techniques in the in-vivo studies.
Keywords
Diabetes Mellitus, In-vitro Models, Anti-Diabetic Activity, Cell Lines, Assays
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- Allen DD, Caviedes R, Cárdenas AM, Shimahara T, Segura-Aguilar J, Caviedes PA. Cell lines as in-vitro models for drug screening and toxicity studies. Drug DevIndPharm.2005 ;318:757-68.
- Rorsman P, Braun M. Regulation of insulin secretion in human pancreatic islets. Annu Rev Physiol2013 ;75 :155-79.
- Mitic T, Emanueli C. Diabetes mellitus-induced epigenetic signature in vascular cells. Endocr Metab Immune Disord Drug Targets (Formerly Current Drug Targets-Immune, Endocrine and Metabolic Disorders) 2012 ;12:107-17.
- Jyoti Bhardwaj, Rashmi Choudhary, Poonam Sharma. A comparative study on quality of life among type 1 and type 2 diabetes mellitus clients at selected hospitals of district Mohali, Punjab. Asian J. Nursing Education and Research. 2018; 8(1): 152-158.
- Gupta A. , Chaturvedi P., Shrivastava S. K., Dubey P.K. Glitazones for the Treatment of Diabetes Type-2. Asian J. Research Chem. 5(2): February 2012; Page 164-170.
- Anita p Mandare, Neelamdeokar, Smita v patil3, Gaikwad Pandurangb. study of total cholesterol, triacylglycerols, high-density lipoprotein cholesterol in type 2 diabetes mellitus. Asian J Pharm Clin Res 2007 ;10 :116-118.
- DeFronzo RA. From the triumvirate to the ominous octet: a new paradigm for the treatment of type 2 diabetes mellitus. DM2009 ;
- 58:773-95.
- P. Maheshwari, T.S. Shanmugarajan. Gestational Diabetes mellitus effects on Macrosomia and risk of Developing Type 2 Diabetes in Tertiary care hospital. Research J. Pharm. and Tech. 2017, 10(6): 1589-1991.
- Arul Mozhi. D, Vijaya Manickam. R, Mangala Gowri. Gestational Diabetes Mellitus –Prevention by Life Style Modification. Int. J. Adv. Nur. Management 2(3): July-Sept.,2014; Page 177-179.
- Combs-Orme T. Epigenetics and the social work imperative. acronyms2012 ;58:23-30.
- Babaso V. Udugade, Shivaji P. Gawade. Synthesis and type- II anti-diabetic activity of Pyrrolidine-2-carbonitrile derived Ligands in SHR-STZ Animal model. Asian J. Research Chem. 2018; 11(1):159-165.
- Nwauche Kelechi T, Monago Comfort C, Onwuka Franscis. Management of Diabetic with Combined Therapy of Reducdyn and Metformin in Streptozotocin induced Diabetic Male Rats. Research J. Pharmacognosy and Phytochemistry 2013 5(5): 224-235.
- P.M. Patil, P.D. Chaudhari, N.J. Duragkar, P.P. Katolkar. Formulation and Evaluation its Anti-diabetic Activity of Liquid Oral Preparation of Gymnema sylvestre and Stevia rebaudiana and their Combination in Alloxan Diabetic Rats. Research J. Pharm. and Tech.3 (4): Oct.-Dec.2010; Page 1200-1204.
- Rupesh Gautam, S.C. Sharma. Effect of Punica granatum Linn. (Peel) on Blood Glucose Level in Normal and Alloxan- Induced Diabetic Rats. Research J. Pharm. and Tech. 5(2): Feb. 2012; Page 226-227.
- Kumari Anjali Jain. A Mechanistic Approach to Determination of Anti-diabetic activity of Calystegia sepium R.Br. Flowering plants in normal and Streptozotocin induced rats. Asian J. Res. Pharm. Sci. 4(2): April-June 2014; Page 55-70.
- RB Goswami, Neelima Goswami, P Khare, S Choudhary, AK Pathak. Evaluation of Anti-Diabetic Activity of Leaves and Fruits of Ficus religiosa Linn. Research J. Pharmacognosy and Phytochemistry 2010; 2(1): 61-63.
- Kodama HA, Amagai Y, Sudo H, Kasai S, Yamamoto S. Establishment of a clonal osteogenic cell line from newborn mouse calvaria. J Oral Biosci1981 ;23:899-901.
- Skelin M, Rupnik M, Cencic A. Pancreatic beta cell lines and their applications in diabetes mellitusmellitus research. ALTEX 2010; 27 :105-13.
- Fröde TS, Medeiros YS. Animal models to test drugs with potential Anti-Diabetic activity. J Ethnopharmacol 2008 ;115:173-83.
- Robertson RP, Zhang HJ, Pyzdrowski KL, Walseth TF. Preservation of insulin mRNA levels and insulin secretion in HIT cells by avoidance of chronic exposure to high glucose concentrations. Eur J Clin Invest 1992; 90:320.
- Gulati V, Gulati P, Harding IH, Palombo EA. Exploring the anti-diabetic potential of Australian Aboriginal and Indian Ayurvedic plant extracts using cell-based assays. BMC Complement AlternMed 2015; 15:8.
- Cui Z, Chen X, Lu B, Park SK, Xu T, Xie Z, Xue P, Hou J, Hang H, Yates JR, Yang F et al Preliminary quantitative profile of differential protein expression between rat L6 myoblasts and myotubes by stable isotope labeling with amino acids in cell culture. Proteomics. 2009 :1274-92.
- Rojo LE, Ribnicky D, Logendra S, Poulev A, Rojas-Silva P, Kuhn P, Dorn R, Grace MH, Lila MA, Raskin I. In-vitro and in vivo anti-diabetic effects of anthocyanins from Maqui Berry (Aristoteliachilensis). Food Chem2012; 131 :387-96.
- Kozma L, Baltensperger K, Klarlund J, Porras A, Santos E, Czech MP. The Rassignaling pathway mimics insulin action on glucose transporter translocation. Proc. Natl. Acad. Sci. U.S.A 1993 ;90:4460-4.
- Moerman EJ, Teng K, Lipschitz DA, Lecka‐Czernik B. Aging activates adipogenic and suppresses osteogenic programs in mesenchymal marrow stroma/stem cells: the role of PPAR‐γ2 transcription factor and TGF‐β/BMP signaling pathways. Aging cell 2004 ;3:379-89.
- Cheng JH, She H, Han YP, Wang J, Xiong S, Asahina K, Tsukamoto H. Wnt antagonism inhibits hepatic stellate cell activation and liver fibrosis.Am. J. Physiol. Gastrointest. Liver Physiol2008 ;294: 39-49.
- Castilho-Fernandes A, de Almeida DC, Fontes AM, Melo FU, Picanço-Castro V, Freitas MC, Orellana MD, Palma PV, Hackett PB, Friedman SL, Covas DT. Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells. Exp Mol Pathol. 2011 ;91:664-72.
- Vishnu Priya J, Srinivasa Rao A. Evaluation of anticancer activity of Tridax procumbens leaf extracts on A549 and hep g2 cell lines. Asian J Pharm Clin Res.2015;8:129-132.
- Kelly KA, Tanaka S, Baron R, Gimble JM. Murine bone marrow stromally derived BMS2 adipocytes support differentiation and function of osteoclast-like cells in vitro. Endocrinology. 1998 ;139:92-101.
- Begum MS, Aruna A, Sivakumar T, Premanand C, Sribhuaneswari MC. Invitro Cytotoxic Activity On Ethanolic Extracts Of Leaves Of Ipomoea Pes-Tigridis (Convolulaceae) Against Liver Hepg2 Cellline 2015;3: 1778–1784.
- Qiu GH, Xie X, Xu F, Shi X, Wang Y, Deng L. Distinctive pharmacological differences between liver cancer cell lines HepG2 and Hep3B. Cytotechnology. 2015;67:1-2.
- Dilger JP, Liu Y. Desensitization of acetylcholine receptors in BC3H-1 cells. Pflugers Arch. 1992 ;420:479-85.
- Taubman MB, Smith CW, Izumo S, Grant JW, Endo T, Andreadis A, Nadal-Ginard B. The expression of sarcomeric muscle-specific contractile protein genes in BC3H1 cells: BC3H1 cells resemble skeletal myoblasts that are defective for commitment to terminal differentiation. J. Cell Biol. 1989; 108:1799-806.
- Balasubramanian R, Robaye B, Boeynaems JM, Jacobson KA. Enhancement of glucose uptake in mouse skeletal muscle cells and adipocytes by P2Y6 receptor agonists. PloS one. 2014; 9:116-203.
- Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods1986 ;2:271-7.
- Lafontan M. Advances in adipose tissue metabolism. Int J Obes. 2008 ;32: 39-51.
- Zelles L. Fatty acid patterns of phospholipids and lipopolysaccharides in the characterization of microbial communities in soil: a review. Biol Fertil Soils. 1999; 29:111-29.
- Shirazi-Beechey SP, Davies AG, Tebbutt K, Dyer J, Ellis A, Taylor CJ, Fairclough P, Beechey RB. Preparation and properties of brush-border membrane vesicles from human small intestine. Gastroenterology. 1990; 3:676-85.
- Lau CH, Chan CM, Chan YW, Lau KM, Lau TW, Lam FC, Che CT, Leung PC, Fung KP, Ho YY, Lau CB et al. In-vitro Anti-Diabetic activities of five medicinal herbs used in Chinese medicinal formulae. Phytother Res Title(s)2008; 22:1384-8.
- Fujimura H, Murakami N, Miwa S, Aruga C, Toriumi W. The suitability of rat hepatoma cell line H4IIE for evaluating the potentials of compounds to induce CYP3A23 expression. Exp Toxicol Patho. 2012 ;64: 527-33.
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