Research Journal of Pharmacy and Technology

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Screening of Enzymes from Actinomycetes and Fungi isolated from Plastic Dumped Soil


Affiliations
1 Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-600117, Tamilnadu, India
     

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Enzymes as catalysts play a major role in day to day lives by catalyzing major biochemical and metabolic interconversions. Microbes are chief source of enzymes and synthesize them for the production of secondary metabolites. These enzymes can be exploited to develop many novel functions. In this study, actinomycetes and fungi were isolated from plastic waste dumped soil. A total of 35 actinomycetes and 15 fungal species were isolated from first level screening. Among them 5 actinomycetes and 3 fungal strains that showed better growth were chosen for further enzyme screening and characterization studies. These strains were screened for four enzymes viz. cellulase, protease, xylanase and glucosidase production. Actinomycetes showed xylanase and cellulase producing ability. The fungal strain in addition to the production of xylanase, protease enzymes also showed cellulase production. One actinomycetes and fungal strain that showed multienzyme production were subjected to further biochemical and morphological characterization. From morphological observation, actinomycetes revealed a creamy white, long spored species with some producing a light yellow pigmentation. The fungal strains showed black spores at the centre with surrounding white filaments. The fungal strain was also confirmed by visualizing spores and hyphae under the microscope. The actinomycetes were subjected to various biochemical tests. The tests revealed the strain isolated was an actinomycete. In future, these fungi and actinomycete can be exploited to produce the enzymes in large scale which can be screened for their ability to degrade plastic.

Keywords

Soil, Plastic, Actinomycetes, Fungi, Protease, Cellulase, Glucosidase, Xylanase.
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  • Warma S, Srinivasan M. Antimicrobial Activity of Actinomycetes Isolated from Different soil samples of Vellore Region. Research Journal of Pharmacy and Technology. 2018; 11(7):3123-7.

  • Pranay J, Pundir RK. Antagonistic activity of soil fungal metabolite against Streptococcus mutans strains. Research Journal of Pharmacy and Technology. 2010; 3(2):417-9.

  • Walia A, Guleria S, Mehta P, Chauhan A, Parkash J. Microbial xylanases and their industrial application in pulp and paper biobleaching: a review. 3 Biotech. 2017; 7(1):11.

  • Kumar D, Kumar SS, Kumar J, Kumar O, Mishra SV, Malyan S, Kumar R. Xylanases and Their Industrial Applications: A Review. Biochemical and Cellular Archives. 2017; 17(1):353-60.

  • Sitoke A, Chopra RS, Kumar PG, Chopra C. Identification and Characterization of an Alkalophilic Protease from Bacillus Mycoides strain isolated from Industrial Soil of Phagwara, India. Research Journal of Pharmacy and Technology. 2017; 10(10):3435-8.

  • Furhan J, Sharma S. Microbial alkaline proteases: Findings and Applications. International Journal of Pharmacy and Pharmaceutical Sciences.. 2014; 4:823-34.

  • Bharathi V, Firdous J, Mona R, Muhamad N. Efficacy of Cellulose Degrading Bacteria from Soil in Production of Cellulase from Corn Waste. Research Journal of Pharmacy and Technology. 2018; 11(9):4024-8

  • Behera BC, Sethi BK, Mishra RR, Dutta SK, Thatoi HN. Microbial cellulases–Diversity & biotechnology with reference to mangrove environment: A review. Journal of Genetic Engineering and Biotechnology. 2017; 15(1):197-210.

  • Ahmed A, Nasim FU, Batool K, Bibi A. Microbial β-glucosidase: sources, production and applications. Journal of Applied & Environmental Microbiology. 2017; 5(1):31-46.

  • Singh G, Verma AK, Kumar V. Catalytic properties, functional attributes and industrial applications of β-glucosidases. 3 Biotech. 2016; 6(1):3.

  • Liu Y, Tortora G, Ryan ME, Lee HM, Golub LM. Potato dextrose agar antifungal susceptibility testing for yeasts and molds: evaluation of phosphate effect on antifungal activity of CMT-3. Antimicrobial agents and chemotherapy. 2002; 46(5):1455-61.

  • Mohseni M, Norouzi H, Hamedi J, Roohi A. Screening of antibacterial producing actinomycetes from sediments of the Caspian Sea. International journal of molecular and cellular medicine. 2013; 2(2):64.

  • Nummi M, Perrin JM, Niku-Paavola ML, Enari TM. Measurement of xylanase activity with insoluble xylan substrate. Biochemical Journal. 1985; 226(2):617-20.

  • Naggie S, Hu YC, Pulliam-Holoman TR, Bentley WE. Substrate (gelatin) gel electrophoretic method for analysis of protease activity in insect (Sf-9) cells. Biotechnology techniques. 1997; 11(5):297-300.

  • Ko IK, Kato K, Iwata H. A thin carboxymethyl cellulose culture substrate for the cellulase-induced harvesting of an endothelial cell sheet. Journal of Biomaterials Science, Polymer Edition. 2005; 16(10):1277-91.

  • Chen H, Yan X, Lin W, Zheng L, Zhang W. A New Method for Screening a-Glucosidase Inhibitors and Application to Marine Microorganisms. Pharmaceutical biology. 2004; 42(6):416-21.

  • Thiruvengadam S, Jeevanantham S, Kamalesh R, Hamsini S, Kamali TB, Karishma S, Jayalakshmi H. Designing and Development of Rice water based crude media and it’s application in fungal isolation and enzyme production. Research Journal of Pharmacy and Technology. 2018; 11(9):1-3

  • Dhananjeyan V, Selvan N, Dhanapal K. Isolation, Characterization, Screening and Antibiotic Sensitivity of Actinomycetes from Locally(Near MCAS) Collected Soil Samples. Journal of Biological Sciences. 2010; 10(6):514-9.

  • Beveridge TJ. Use of the Gram stain in microbiology. Biotechnic & Histochemistry. 2001; 76(3):111-8.

  • Hemraj V, Diksha S, Avneet G. A review on commonly used biochemical test for bacteria. Innovare Journal of Life Sciencesi. 2013; 1(1):1-7.


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  • Screening of Enzymes from Actinomycetes and Fungi isolated from Plastic Dumped Soil

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Authors

Sowmya Hari
Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-600117, Tamilnadu, India

Abstract


Enzymes as catalysts play a major role in day to day lives by catalyzing major biochemical and metabolic interconversions. Microbes are chief source of enzymes and synthesize them for the production of secondary metabolites. These enzymes can be exploited to develop many novel functions. In this study, actinomycetes and fungi were isolated from plastic waste dumped soil. A total of 35 actinomycetes and 15 fungal species were isolated from first level screening. Among them 5 actinomycetes and 3 fungal strains that showed better growth were chosen for further enzyme screening and characterization studies. These strains were screened for four enzymes viz. cellulase, protease, xylanase and glucosidase production. Actinomycetes showed xylanase and cellulase producing ability. The fungal strain in addition to the production of xylanase, protease enzymes also showed cellulase production. One actinomycetes and fungal strain that showed multienzyme production were subjected to further biochemical and morphological characterization. From morphological observation, actinomycetes revealed a creamy white, long spored species with some producing a light yellow pigmentation. The fungal strains showed black spores at the centre with surrounding white filaments. The fungal strain was also confirmed by visualizing spores and hyphae under the microscope. The actinomycetes were subjected to various biochemical tests. The tests revealed the strain isolated was an actinomycete. In future, these fungi and actinomycete can be exploited to produce the enzymes in large scale which can be screened for their ability to degrade plastic.

Keywords


Soil, Plastic, Actinomycetes, Fungi, Protease, Cellulase, Glucosidase, Xylanase.

References