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Diagnosis of Human Papilloma virus in Colorectal Cancer and Inhibition by Gamma Radiation


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1 Department of Laboratory Investigation, Kufa University, Najaf, Iraq
     

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Human Papilloma virus was isolated from different age groups from 40 upto 71 years for both gender. The samples were diagnosed by RT-PCR. Positive cases of human Papilloma virus were forty represent (63.49%) and twenty two represent (36.50%) negative cases. Isolation process of Human Papillomavirus was successfully performed on chicken embryo fibroblast cell culture for 4th passage for suspected samples obtained from (colon organ) as well as isolate from chick embryo specific pathogen free (SPF). It was found that at the tissue culture infective dosage (TCID50/0.1 ml) was (107.8) of virus. The gamma irradiation that dosage used for inactivating of the purified virus which was extracted from allantoic fluid and tissue culture was (6.594) mSv/h. This revision intended to gauge the prevalence of HPV cannon and viral incorporation in colorectal tissues in imperative to estimate its presumed role in colorectal cancer and inhibition viral by gamma radiation.

Keywords

Allantoic Fluid, Ulcerative Colitis, Crohn's Disease.
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  • Diagnosis of Human Papilloma virus in Colorectal Cancer and Inhibition by Gamma Radiation

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Authors

Fadyia Mahdi Muslim Alameedy
Department of Laboratory Investigation, Kufa University, Najaf, Iraq

Abstract


Human Papilloma virus was isolated from different age groups from 40 upto 71 years for both gender. The samples were diagnosed by RT-PCR. Positive cases of human Papilloma virus were forty represent (63.49%) and twenty two represent (36.50%) negative cases. Isolation process of Human Papillomavirus was successfully performed on chicken embryo fibroblast cell culture for 4th passage for suspected samples obtained from (colon organ) as well as isolate from chick embryo specific pathogen free (SPF). It was found that at the tissue culture infective dosage (TCID50/0.1 ml) was (107.8) of virus. The gamma irradiation that dosage used for inactivating of the purified virus which was extracted from allantoic fluid and tissue culture was (6.594) mSv/h. This revision intended to gauge the prevalence of HPV cannon and viral incorporation in colorectal tissues in imperative to estimate its presumed role in colorectal cancer and inhibition viral by gamma radiation.

Keywords


Allantoic Fluid, Ulcerative Colitis, Crohn's Disease.

References