Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Synergistic Antifungal Activity of Bioactive Phytochemical in Combination with Standard Antifungal Drugs


Affiliations
1 Assam Down Town University, Panikhaiti, Gandhinagar, Guwahati-26 Assam, India
2 Columbia Institute of Pharmacy, Tekari, Raipur C.G., India
     

   Subscribe/Renew Journal


The research work was mainly designed to determine the antifungal activity of curcumin, a potent bioactive phytoconstituent, obtained from Curcuma longa and to explore the possibilities of its use as a combination with commercially available synthetic antifungal drugs for better therapeutic actions. Fluconazole and itraconazole were used as a model drug. Different combinations of curcumin with fluconazole as well as itraconazole was prepared and subjected to an antifungal screening study. The antifungal screening was carried out using Candida albicans fungal strain. The MIC of fluconazole, itraconazole and curcumin was found to be in the range of 32 μg/ml to 64 μg/ml, 8 μg/ml to 32μg/ml and 64 μg/ml to 256 μg/ml respectively. Further, the results of the invitro antifungal study performed based on the comparative zone of inhibition measurement of the prepared combination at a concentration of 10 μg/ml were determined. The result of the study indicates that the presence of curcumin significantly increases the antifungal capacity of both fluconazole and itraconazole. The Fractional inhibitory concentration index was measured, and the data thus obtained, states that the increased antifungal activity may be observed due to synergistic or additive effects. Further, the topical sensitivity of the optimized combinations was determined using rabbit vaginal model and were found to be free from any major sign of sensitivity as per as histopathological study concern.

Keywords

FICI, Natural Antifungal, Candida albican, Curcumin, Antifungal Combination.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Umme H, Hosakote G S, Vaghela R, Osmani RA and Shrivastava A, Candidiasis: A fungal infection- current challenges and progress in prevention and treatment, Infect. Disord.: Drug Targets, 2015, 15, 42-52

  • Choudhury A, Roy A, Bahadur S, and Saha S, Phytoconstituent based mucoadhesive antifungal vaginal formulation: An effective and innovative approach, Biosci. Biotechnol. Res. Commun., 2016, 9(4), 694-701

  • Denning D W and Hope WW, Therapy for fungal diseases: Opportunities and priorities, trends in microbiology.(IN PRESS)

  • Wagner H, and Merzenich G U, Synergy research: Approaching a new generation of phyto pharmaceuticals. J. Nat. Rem.,2009, 9(2), 121 – 141

  • Matlock K, Berlow N, Keller C, and Pal R, Combination therapy design for maximizing sensitivity and minimizing toxicity, BMC Bioinf., 2017, 18(4),17-29.

  • Badiee P and Hashemizadeh Z, Opportunistic invasive fungal infections: Diagnosis and clinical management, Indian J Med Res, 2014, 139,195-204.

  • Mohapatro SK, Dandapat MC and Padhi NC, Toxicity and sideeffects of combination chemohormonal therapy of advanced breast cancer, J Indian Med Assoc., 1992, 90(2):39-42.

  • Chanda S and Rakholiya K, Combination therapy: Synergism between natural plant extracts and antibiotics against infectious diseases. Science against microbial pathogens: communicating current research and technological advances. Formatex, 2011, 520-529

  • Doble M, Hemaiswarya S and Kruthiventi A K, Synergism between natural products and antibiotics against infectious diseases, Phytomedicine, 2008, 15, 639–652

  • Barbier R, Coppo E, Marchese A, Daglia M, Sánchez Eet al., Phytochemicals for human disease: An update on plant-derived compounds antibacterial activity, Microbiol. Res., 2017, 196, 4468

  • Cowan M M, Plant Products as Antimicrobial Agents, Clin. Microbiol. Rev., 1999,12(4), 564–582

  • Choudhury A, Verma A, Sinha D, Shau S and Roy A, Development and characterization of topical phyto-formulation for antifungal activity, J. Appl. Pharm. Res.2014, 1(2), 18-21.

  • Choudhury A, Roy A, Bahadur S, and Saha S, Preparation and evaluation of plant bioactive phytochemical based bioadhesive antifungal vaginal formulation. Indo Am. J. Pharm. Sci., 2016, 3 (12), 1673-1681

  • Septama AW and Pharkphoom P, Synergistic effect of artocarpin on antibacterial activity of some antibiotics against methicillin resistant Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli, Pharm Biol, 2016, 54(4), 686–691

  • Pereira A F, Gomes A S G, Curvelo J A R R and Soares MA, Curcumin acts synergistically with fluconazole to sensitize a clinical isolate of Candida albicans showing a MDR phenotype. Med. Mycol., 2012, 50, 26–32.

  • Fratinia F, Mancinia S, Turchia B, Frisciaa E, Pistellib Let al., A novel interpretation of the Fractional Inhibitory Concentration Index: The case Origanum vulgare L. and Leptospermum scoparium J. R. Forst essential oils against Staphylococcus aureus strains, Microbiol. Res.2017,195, 11–17.

  • Mehta S, Verstraelen H, Peremens K, Villeirs G, and Vermeire S, Vaginal distribution and retention of a multiparticulate drug delivery system assessed by gamma scintigraphy and magnetic resonance imaging. Int. J. Pharm.,2012, 426, 44-53

  • Rabindranath P, Chakraborty M, Rabindra D, and Gupta BK. Invitro In-vivo correlation (IVIVC) study of leflunomide loaded microspheres. Int. J. Pharm. Sci.,2009, 1, 165-170.

  • D’Cruz O J, Waurzyniak B and Uckun F M, Mucosal toxicity studies of a gel formulation of native pokeweed antiviral protein. Toxicol. Pathol.,2004, 32, 212–221

  • TehC H, Nazni WA, Nurulhusna A H, Norazah A and Lee H L, Determination of antibacterial activity and minimum inhibitory concentration of larval extract of fly via resazurin-based turbidometric assay, BMC Microbiol., 2017, 17(36), 1-8


Abstract Views: 237

PDF Views: 0




  • Synergistic Antifungal Activity of Bioactive Phytochemical in Combination with Standard Antifungal Drugs

Abstract Views: 237  |  PDF Views: 0

Authors

Ananta Choudhury
Assam Down Town University, Panikhaiti, Gandhinagar, Guwahati-26 Assam, India
Suman Saha
Columbia Institute of Pharmacy, Tekari, Raipur C.G., India
Sanjib Bahadur
Columbia Institute of Pharmacy, Tekari, Raipur C.G., India
Amit Roy
Columbia Institute of Pharmacy, Tekari, Raipur C.G., India

Abstract


The research work was mainly designed to determine the antifungal activity of curcumin, a potent bioactive phytoconstituent, obtained from Curcuma longa and to explore the possibilities of its use as a combination with commercially available synthetic antifungal drugs for better therapeutic actions. Fluconazole and itraconazole were used as a model drug. Different combinations of curcumin with fluconazole as well as itraconazole was prepared and subjected to an antifungal screening study. The antifungal screening was carried out using Candida albicans fungal strain. The MIC of fluconazole, itraconazole and curcumin was found to be in the range of 32 μg/ml to 64 μg/ml, 8 μg/ml to 32μg/ml and 64 μg/ml to 256 μg/ml respectively. Further, the results of the invitro antifungal study performed based on the comparative zone of inhibition measurement of the prepared combination at a concentration of 10 μg/ml were determined. The result of the study indicates that the presence of curcumin significantly increases the antifungal capacity of both fluconazole and itraconazole. The Fractional inhibitory concentration index was measured, and the data thus obtained, states that the increased antifungal activity may be observed due to synergistic or additive effects. Further, the topical sensitivity of the optimized combinations was determined using rabbit vaginal model and were found to be free from any major sign of sensitivity as per as histopathological study concern.

Keywords


FICI, Natural Antifungal, Candida albican, Curcumin, Antifungal Combination.

References