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The Efficacy of Insecticide Indoxacarb (Avaunt) against Larval Stage of House Fly Musca domestica L.


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1 Institute of Technology-Middle Technical University/Baghdad, Iraq
     

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Indoxacarb is a recently presented oxadiazine insecticide with activity against an extensive range of pests, including house flies. The efficacy of Avaunt (Indoxacarb 15% EC) on newly ecdysed 2nd and 3rd instar Musca domestica L. larvae was evaluated. Chemical concentrations tested fluctuated from 20 to 100 ppm of indoxacarb, that indoxacarb Observations revealed caused quick mortality at doses >50 ppm. At concentrations below 50 ppm, it acquired longer for indoxacarb to cause 100% mortality. In the food treated 100% mortality for the 2nd and 3rd instar larvae caused within 48 to 144 hrs. and 72 to 144 hrs. respectively, as well as in spray treated caused within 48 to 144 hrs. and 96 to 144 hrs. respectively. Indicated that ingesting indoxacarb was highly toxic to the second and third instars of M. domestica. The LC590 values of indoxacarb exceeded the highest concentrations tested 100 ppm. LC50 for 2nd and 3rd larvae exposed to food treated were 19.18, 10.78 and 10.08 and 14.281, 12.40 and 9.876 ppm at 24, 48 and 72 hrs respectively. LC50 for 2nd and 3rd larvae exposed to spray treated were 23.54, 14.73 and 9.52 and 23.564, 9.330 and 7.912 at 24, 48 and 72 hrs. Results of the conducted bioassay showed that 2nd instars were more vulnerable than the 3rd instars as the LC50 and LC90 values.

Keywords

Musca domestica, Indoxacarb, Avaunt, Insecticide, Larva, LC50, LC90, Sodium Channel.
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  • Malik, A; Singh, N. and Satya, S. (2007). House fly (Musca domestica): a review of control strategies for a challenging pest. J Environ Sci Heal B 42:453–469.
  • Taylor, D.B; Moon, R.D. and Mark, D.R. (2012). Economic impact of stable flies (Diptera: Muscidae) on dairy and beef cattle production. J Med Entomol 49:198-209.
  • Alam, M.J. and Zurek, L (2004). Association of Escherichia coli O157:H7 with houseflies on a cattle farm. Appl Environ Microbiol 70:7578-7580.
  • Talley, J.L; Wayadande, A.C; Wasala, L.P; Gerry, A.C, Fletcher, J; DeSilva, U. and Gilliland, S.E. (2009). Association of Escherichia coli O157:H7 with filth flies (Muscidae and Calliphoridae) captured in leafy greens fields and experimental transmission of E. coli O157:H7 to spinach leaves by house flies (Diptera: Muscidae). J Food Protect 72:1547-1552.
  • Machtinger, E.T; Leppla, N.C. and Sanders, C.S. (2012). Pest management perceptions and practices for equine farms in north and central Florida. University of Florida, Institute of Food and Agricultural Sciences, Gainesville, FL. IFAS Publication ENY-2028 http://edis.ifas.ufl.edu/in983.
  • Ferguson, H; Neal, S.O; Walsh, D; Galvin, K; Vásquez, V. and Yost, M. (2014). Fly control news to moo about: survey of pest management practices on Washington dairy farms April 2014. Washington State University Extension, Prosser, WA. http://wastatedairy.com/wpcontent/uploads/2014/04/WSU-Extension-dairy-survey-results-Apr2014forprint.pdf.
  • Kristensen, M. and Jespersen, J.B. (2004). Susceptibility of spinosad in Musca domestica (Diptera: Muscidae) field populations. J Econ Entomol 97:1042–1048.
  • Kaufman, P.E; Nunez, S.C; Mann, R.S; Geden, C.J. and Scharf, M.E. (2010). Nicotinoid and pyrethroid insecticide resistance in houseflies (Diptera: Muscidae) collected from Florida dairies. Pest Manage Sci 66: 290–294.
  • Hinkle, N. C. (2002). Poultry pest management (arthropods). In: D. Pimentel (ed.), Encyclopedia of Pest Management. Marcel Dekker, Inc., New York, Pp. 657-660. Steenberg, T and Jespersen, J. B. (2002). Control of houseflies and ‘filth’ flies, In: D. Pimentel (ed.), Encyclopedia of Pest Management. Marcel Dekker, Inc., New York, Pp. 144-147.
  • Winpisinger, K. A., Frenetic, A. K., Berry, R. L. and Moeschberger, M. L. (2005). Spread of Musca domestica (Diptera: Muscidae), from two caged layer facilities to neighboring residence in rural Ohio. Journal Medical Entomology 42: 732-738.
  • Lee, C. Y., Zairi. J., Yap, H. H., and Chong, N. L. (2003). Urban pest control: A Malaysian perspective. Vector Control Research Unit, University Science Malaysia, Malaysia. Pp. 87-98.
  • Pap, L., and R. Farkas. 1994. Monitoring of resistance of insecticides in house fly (Musca domestica) populations in Hungary. Pestic. Sci. 40: 245-258.
  • Scott, J. G., Alefantis, T. G., Kaufman, P. E., and Rutz, D. A. (2000). Insecticide resistance in house flies from caged layer poultry facilities. Pest Manage. Sci. 56: 147-153.
  • Deacutis, J. M., Leichter, C. A., Gerry, A. C. Rutz, D. A., Watson, W. D., Geden, C. J., and Scott, J. G. (2006). Susceptibility of field collected house flies to spinosad before and after a season of use. J. Agric. Urban Entomol. 23: 105-110.
  • Butler, S. M., Gerry, A. C. and Mullens, B. A. (2007). House fly (Diptera: Muscidae) activity near baits containing (Z)-9-tricosene and efficacy of commercial toxic fly baits on a southern California dairy. J. Econ. Entomol. 100: 1489-1495.
  • Moon, R.; Hinton, J.; O’Rourke, D.; and Schmidt, D. (1994). Nutritional value of fresh and composted poultry manure for house fly (Diptera: Muscidae) larvae. J. Econ. Entomol. 1308–1317.
  • Scott, J.G. (1998). Toxicity of Spinosad to Susceptible and Resistance Strains of House Flies, Musca domestica. Pestic. Sci. 54, 131–133.
  • Keiding, J. (1975). Problems of housefly (Musca domestica) control due to multi-resistance to insecticides. J. Hyg. Epid. Microbiol. Immunol. 19, 340–355.
  • Farnham, A.W. (1977). Genetics of resistance of houseflies (Musca domestica) to pyrethroids I knockdown resistance. Pestic. Sci. 8, 631.
  • Sawicki, R.M.; Farnham, A.W.; Denholm, I.; and O’Dell, K. (1981). Housefly resistance to pyrethroids in the vicinity of Harpenden. Proc. Brit. Crop Protect. Conf. 609–613.
  • Salgado VL. (1990). Mode of action of insecticidal dihydropyrazoles: selective block of impulse generation in sensory nerves. Pestic Sci.; 28:389–411.
  • Salgado VL. (1992). Slow voltage-dependent block of sodium channels in crayfish nerve by dihydropyrazole insecticides. Mol Pharmacol.; 41:120–126.
  • Wing, K.D.; Andaloro, J.T.; McCann, S.F.; and Salgado, V.L. (2005). Indoxacarb and the sodium channel blocker insecticides: chemistry, physiology, and biology in insects. In: Gilbert, LI.; Iatrou, K.; Gill, SS., editors. Comprehensive Molecular Insect Science. Vol. 6. Elsevier; New York: p. 30-53.
  • Lahm, G. P., McCann, S. F., Harrison, C. R., Stevenson, T. M., and Shapiro, R., (2001). Evolution of the sodium channel blocking insecticides: the discovery of indoxacarb, pp. 20- 34. In: Agrochemical discovery insect, weed, and fungal control (BAKER D. R., UMETSU N. K., Eds), Symposium Series 774. American Chemical Society, Washington, DC, USA.
  • Wing, K. D., Sacher, M., Kagaya, Y., Tsurubuchi, Y., Mulderig, l., Connair, M., and Schnee, M., (2000). Bioactivation and mode of action of the oxadiazine indoxacarb in insects. - Crop Protection, 19: 537-545.
  • Dinter A., and Wiles J. A., (2000). Safety of the new DuPont insecticide indoxacarb to beneficial arthropods: an overview. - Bulletin OILB/SROP, 23: 149-156.
  • N’Guessan, R., Corbel, V., Bonnet, J., Yates, A., Asidi, A., Boko, P., Odjo, A., Akogbéto, M., and Rowland, M., (2007). Evaluation of indoxacarb, an oxadiazine insecticide for the control of pyrethroidresistant Anopheles gambiae (Diptera: Culicidae). - Journal of Medical Entomology, 44: 270-276.
  • Habachi, W., Bensafi, H., Adjami, Y., Ouakid, M. L., Farine J. P., and Everaertz C., (2009). Spinosad affects chemical communication in the German cockroach, Blattella germanica (L). - Journal of Chemical Ecology, 35: 1423-1426.
  • Gondhalekar, A. D, Song, C., and Scharf, M. E., (2011). Development of strategies for monitoring indoxacarb and gel bait susceptibility in the German cockroach (Blattodea: Blattellidae). - Pest Management Science, 67: 262-270.
  • Mahmoudvand, M., Abbasipour, H., Garjan, A. S., and Bandani, A. R., (2011). Sublethal effects of indoxacarb on the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Yponomeutidae). - Applied Entomology and Zoology, 46: 75-80.
  • Wing, K. D., M. E. Schnee, M. Sacher, and M. Connair. (1998). A novel oxadiazine insecticide is bio-activated in lepidopteran larvae. Arch. Insect Biochem. Physiol. 37: 91- 103.
  • Sugiyama, S., Y. Tsurubuchi, A. Karasawa, K. Nagata, Y. Kono, and T. Shono. (2001). Insecticidal activity and cuticular penetration of indoxacarb and its N decarbomethoxyllated metabolite in organophosphorus insecticide- resistant and susceptible strains of the housefly, Musca domestica (L.). J. Pestic. Sci. 26: 117-120.
  • Lapied, B., Grolleau, D. B. and Sattelle, B. (2001). Indoxacarb, an oxadiazine insecticide blocks insect neuronal sodium channels. Br. J. Pharmacol. 132: 587-595.
  • Chair, Y., and Lee, C. Y., (2010). Insecticide resistance profiles and synergism in field populations of German cockroach, Blattella germanica (L.) (Dictyoptera: Blattellidae) from Singapore. - Journal of Economic Entomology, 103: 460-471.
  • Khan, H.A.A., Akram, W, and Shad, S.A. (2013). Resistance to conventional insecticides in Pakistani populations of Musca domestica L. (Diptera: Muscidae): a potential ectoparasite of dairy animals. Ecotoxicol. 22(3):522-7.
  • Sumitomo Chem. Co. (1997). Dep. of Pesticide Res. Lab. Rearing methods of Insect Pests. Tokyo, pp.7.
  • McKinley, N. Kijima, S; Cook, G. and Sherrod, D. (2002). avaunt (indoxacarb): a new mode of action insecticide for control of several key orchard pests Proceedings of the 76th Annual Western Orchard Pest & Disease Management Conference 9-11 January 2002, Hilton Hotel, Portland, OR Publ. By Washington State Univ., Pullman, Washington Chemical Control/New Products.
  • Kaufman, P.E, Gerry, A.C, Rutz. D.A, and Scott, J.G. (2006) Monitoring susceptibility of house flies (Musca domestica L.) in the United States to imidacloprid. J Agric Urban Entomol 23:195–200.
  • Scott, J.G. (1998). Toxicity of Spinosad to Susceptible and Resistance Strains of House Flies, Musca domestica. Pestic. Sci., 54, 131–133.
  • Finney, D.J (1971). Probit analysis. Cambridge University Press, Cambridge, pp 3–1971.
  • Gamil, W.E. Mariy, F.M. Youssef, L.A. and Abdel Halim, S.M. (2011). Effect of Indoxacarb on some biological and biochemical aspects of Spodoptera littoralis (Boisd.) larvae. Annals of Agricultural Science 56(2), 121–126.
  • Sabri, M. A., Aslam, M. S., Hussain, D. and Saleem, M. (2016). Evaluation of lethal response of biorational insecticides against Spodoptera litura (Lepidoptera: Noctuidae) Journal of Entomology and Zoology Studies; 4(4): 270-274.
  • Alves, S.N.; Serra'o, J.E.; Mocelin, G., and De Melo, A.L. (2004). Effect of ivermectin on the life cycle and larval fat body of Culex quinquefasciatus. Brazilian archives of biology and technology. 47(3):439-443.
  • Halperm, M.; Gasith, A.; Bresler, V.M. And Broza, M. (2002). The protective nature of Chironomus luridus larval tubes against copper sulphate. J. of Insect Science, 2:8.
  • Saini, R.K, Kumar, S, Sharma, S.S. (2005). Evaluation of insecticides against Spodoptera litura (Fab.) attacking cotton. J Cotton Res. Dev.; 19(2):273-276.
  • Andaloro, J. T., Wing, K. D. J. H. and Green, E. Lang, B. (2000). Steward™ dispersion and cotton leaf interactions: impact on cotton insect pests and safety to beneficial arthropods, pp. 939-940 In Proc. 2000 Beltwide Cotton Conf., National Cotton Council, Memphis, TN.
  • Asid, A.N., Al-Ghamdi, K. M., Mangoud A.A.H, Al Asiry, K. Alkenani, N.A. and Anwar, Y. (2017). Synergistic effect of insecticides on the larvae and adults of housefly, Musca domestica L. Journal of Entomology and Zoology Studies; 5(4): 899-905.
  • Vishal, M., Rahman, S.M.A., Kuldeep, S., Nayak, S.K., (2005). Antifeedant effects of sub-lethal concentrations of some novel insecticides against tobacco caterpillar, Spodoptera litura (Fab.). J. Plant Protect. Environ. 2 (2), 118–122.
  • Misbah-ul-haq1, M., Khan, I. A., Farid, A., Ullah, M., Gouge, D. H. Paul B. Baker, P. B. (2016). Efficacy of indoxacarb and chlorfenapyr against Subterranean termite Heterotermes indicola (Wasmann) (Isoptera: Rhinotermitidae) in the laboratory Türk. Entomol. derg.40 (3): 227-241.
  • Ahmed, S., Rasool, M. R., Anullah, I. and Rauf, I. (2004). Comparative Efficacy of Some Insecticides Against Helicoverpa armigera Hub. and Spodoptera spp. on Tobacco International Journal of Agriculture &Biology 6 (1) 150-158.
  • EPA. (2000). Pesticide Fact Sheet: Name of Chemical: Indoxacarb. Issued October 30, 2000. Environmental Protection Agency, Washington, D.C.
  • Salgado, V.L. Hayashi, J.H. (2007). Metaflumizone is a novel sodium channel blocker insecticide, Vet. Parasitol. 150:182–189.
  • Song, W., Silver, K.S., Du, Y., Liu, Z., Dong, K., (2011). Analysis of the action of lidocaine on insect sodium channels. Insect Biochem. Mol. Biol. 41 (1), 36–41.
  • Anonymous. (1998). Avaunt, insect control agent, pp. 107. Technical Bulletin H-79164. DuPont, Wilmington, DE.
  • Harder, H. H., Riley, S. L. McCann, S. F. and Irving, S. N. (1996). DPX-M, p. 062: A novel broad-spectrum, environmentally soft, insect control compound. In Proceedings, 1996 Brighton Conference, Brighton, UK.
  • Davidson, EW. (1979). Ultra-structure of mid-gut events in the pathologenesis of Bacillus Sphaericus Strains SSII-I Infections of Cu pipiens quinquefasciatus larvae. Can. J. Microbiol. 25(2): 178-184.
  • Guessan, R. N., Corbel, V., Bonnet, J., Yates, A., Asidi, A., Boko, P., Odjo, A., Akogbe´ to, M. and Rowland, M. (2007). Evaluation of Indoxacarb, an Oxadiazine Insecticide for the Control of Pyrethroid Resistant Anopheles gambiae (Diptera: Culicidae). J. Med. Entomol. 44(2): 270-276.

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  • The Efficacy of Insecticide Indoxacarb (Avaunt) against Larval Stage of House Fly Musca domestica L.

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Authors

Nisreen Sabti Mohammed Ali
Institute of Technology-Middle Technical University/Baghdad, Iraq

Abstract


Indoxacarb is a recently presented oxadiazine insecticide with activity against an extensive range of pests, including house flies. The efficacy of Avaunt (Indoxacarb 15% EC) on newly ecdysed 2nd and 3rd instar Musca domestica L. larvae was evaluated. Chemical concentrations tested fluctuated from 20 to 100 ppm of indoxacarb, that indoxacarb Observations revealed caused quick mortality at doses >50 ppm. At concentrations below 50 ppm, it acquired longer for indoxacarb to cause 100% mortality. In the food treated 100% mortality for the 2nd and 3rd instar larvae caused within 48 to 144 hrs. and 72 to 144 hrs. respectively, as well as in spray treated caused within 48 to 144 hrs. and 96 to 144 hrs. respectively. Indicated that ingesting indoxacarb was highly toxic to the second and third instars of M. domestica. The LC590 values of indoxacarb exceeded the highest concentrations tested 100 ppm. LC50 for 2nd and 3rd larvae exposed to food treated were 19.18, 10.78 and 10.08 and 14.281, 12.40 and 9.876 ppm at 24, 48 and 72 hrs respectively. LC50 for 2nd and 3rd larvae exposed to spray treated were 23.54, 14.73 and 9.52 and 23.564, 9.330 and 7.912 at 24, 48 and 72 hrs. Results of the conducted bioassay showed that 2nd instars were more vulnerable than the 3rd instars as the LC50 and LC90 values.

Keywords


Musca domestica, Indoxacarb, Avaunt, Insecticide, Larva, LC50, LC90, Sodium Channel.

References