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A Comparative Histomorphological and Histochemical Study of the Goblet Cells and Brunner's Glands in the Duodenum of Rabbits and Rats


Affiliations
1 Department of Physiology, Biochemical and Pharmacology/College of Veterinary Medicine/AL-Muthanna University, Iraq
2 Department of Anat. and Hist./College of Veterinary Medicine/AL-Muthanna University, Iraq
3 Dep. of Anatomy/College of Nursing/University of Al-Basra, Iraq
     

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The purpose of this study was to describe, comparison the histological structures, histochemical features and distribution of the goblet cells and Brunner's glands in the duodenum of the rabbits and rats. The study was carried out on Ten samples of duodenum of each of rabbits and rats, immediately after slaughtering. The specimens were divided into cranial, descending and ascending parts. Five specimens was taken from each part of duodenum of each of the rabbits and rats and fixed in 10% formalin for 24 hours and treating by routine histological technique. Sections were stained by Hematoxylin and Eosin, Picric acid stain and Alcian blue PH 2.5 stains. Mean length of crypts of Lieberkuhn and number of each crypts, goblet cells and alveoli of Brunner's glands in duodenum of rabbits were more than that in the duodenum of rats. The mean length and number of crypts of Lieberkuhn was increase toward the last parts of the duodenum in each of the rabbits and rats. Goblet cells were globular cells between the columnar cells in the epithelium lined each of the villi and crypts of Lieberkuhn in the tunica mucosa of the duodenum in rabbits and rats and take positive reaction each of PAS and Alcian blue pH 2.5 stains. The mean number of goblet cells in crypts of Lieberkuhn in duodenum each rabbits and rats were increased toward the jejunum. Brunner’s glands were branched tubuloalveolar glands in the submucosa of each duodenal parts in rabbits, while absent in ascending part of rats duodenum, and these glands in rabbits duodenum were composed of two types of the cells (serous and mucous), while in rats duodenum only mucous cells, and stained with PAS stain only in duodenum of each of the rabbits and rats. The mean number of the alveoli of the Brunner's glands in the cranial part were more than that in other parts and decreased toward ascending part in rabbits duodenum, while absent in ascending part of rats duodenum.

Keywords

Slaughtering, Lieberkuhn, Tubuloalveolar.
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  • Davies, R. R. and Davies, J.A.E. (2003). Rabbit gastrointestinal physiology.Vet Clin Exot Anim 6:139–153.

  • Malley, O. B. (2005): Clinical Anatomy and Physiology of Exotic Species: Structure and function of mammals, birds, reptiles and amphibians. London, UK: Elsevier Saunders.Pp:163-187.

  • Kotze, S.H.; Merwe, E.L. and Ortain, M. J. (2006). The topography and gross anatomy of the gastrointestinal tract of the Cape Dune Mole-rat (Bathyergus suillus). Anatomia Histologia Embryologia. 35 (4): 259-264.

  • Brewer, N.R. (2006). Biology of the rabbit, Journal of the American Association for Laboratory Animal Science 45(1):8-24.

  • Abidu-Figueiredo, M.; Xavier-Silva, B.; Cardinot, T.; Babinski, M.and Chagas, M. (2008). Celiac artery in New Zealand rabbit: anatomical study of its origin and arrangement for experimental research and surgical practice. Pesquisa Vet. Brazil 28(5):237-240.

  • Elnagy, T.M. and Osman, I. (2010). Anatomical study of the postnatal development of the gastrointestinal tract in rabbits, Uni. of Khartoum. J. Vet. Med. and Anim. Prod. 1(2):174‐183.

  • Dellmann, H. D. and Eurell, J. A. (1998): Textbook of veterinary histology. 5th ed. Awoters Kluwer company Philadelphi, Pp: 187 191.

  • Ahlman, H. and Nilsson, O. (2001). The gut as the largest endocrine organ in the body. Ann. Oncol., 12: 63-68.

  • Taylor, A.B. and Anderson, J. H. (1972). Scanning electron microscope observations of mammalian intestinal villi, intervilli floor and crypt tubules, Micron, 3: 43-53.

  • Coutinho, H.B.; Robalinho, T.I.; Coutinho, V.B.; Amorin, A.M.; Almeida, J.R.; Filho, J.T.; Walker, E.; King, G.; Sewell, H.F. and Wakelin, D. (1996). Immunocytochemical demonstration that human duodenal Brunner’s glands may participate in intestinal defence. J. Anat., 189 (1): 193-197.

  • Farkas, I.E. and Gero, G. (1989). The role of Brunner’s glands in the mucosal protection of the proximal part of duodenum. Acta Physiol. Hung., 73(2-3):257-260.

  • Perrin, M.R. and Curtis, B. A. (1980). Comparative morphology of the digestive system of (19) species of Southern African myomorph rodents in relation to diet and evolution. South African Journal of Zoology, 15(1):22-33.

  • Krause, W. J. (2000). Brunner’s glands: a structural, histochemical and pathological profile. Prog. Histochem. Cytochem., 35(4):259367.

  • Moore, B. A.; Kim, D. and Vanner, S. (2000). Neural pathways regulating Brunner’s glands secretion in guinea pig duodenum in vitro. Am. J. Physiol. Gastrointest. Liver Physiol. 279(5): 910-917.

  • Morikawa, Y.; Miyamoto, M. and Okada, T. (1993). Prenatal development of Brunner’s glands in the rat: morphometrical study. Biol. Neonate, 63(4):258-267.

  • Crescenzi, A.; Biscotti, P.; Anemona, L. and Marinozzi, V. (1988). Carbohydrate histochemistry of human Brunner`s glands. Histochemistry, 90: 47-49.

  • Emel, E.; Leven, E.; Aseman, O. and Aytul, K. (2010). Histomorphology of the Brunner gland in the Angora rabbit. Journal of Animal and Veterinary Advances, 9(5): 887-891.

  • Rashmi, A. N.and Prasad, R. (2016). Histological and histochemical observations on Brunner’s Glands of guinea pig, Journal of Dental and Medical Sciences, 15(8): 100-106.

  • Takehana, K. and Abe, M. (1986). Histochemistry of complex carbohydrates in the duodenal. Journal of the College of Dairying, 11: 371-380.

  • Luna, I. G. (1968): Manual of histology staining methods of the armed force institute of pathology.3rd ed. McGraw. Hill book company. New York. Pp: 33, 76 -168.

  • Al-Rawi, K.M.and Kalaf-Allah, I.S. (1980). Design and Analysis Agriculture Experiments. Dar-Al Kutub-Mosul, Iraq. Pp: 65, 95-107.

  • Ahmed, Y. A.; El-hafez, A. A. E. and Zayed, A. E. (2009). Hitological and histochemical studies on the esophagus, stomach and small intestine of varanus niloticus. 2(1):35-48.

  • Sababi, M.; Nilsson, E. and Holm, L. (1995). Mucus and alkali secretion in the rat duodenum: Effects of indomethacin and luminal acid. Gastroenterology, 109: 1526-1534.

  • Ainsworth, M.A.; Koss, M.A.; Hogan, D.L. and Isenberg. J.I. (1995). Higher proximal duodenal mucosal bicarbonate secretion is independent of Brunner`s glands in rats and rabbits. Gastroenterology 109 :1160- 1166.

  • Poddar, S.and Jakob, S. (1979). Mucosubstance histochemistry of Brunner’s glands, pyloric glands and duodenal goblet cells in the ferret. Histochemistry and cell biol. 65: 67–81.

  • Goyal, M. and Pandher, K. (2017). Postnatal development of Brunner glands in rabbit, Int. J. Adv. Res.5 (3): 868-876.

  • Krause, W.J. (1981). Morphological and histochemical observations on the duodenal glands of eight wild ungulate species native to North America. J Anat, 162:167–181.

  • Krause W.J. (1975). The duodenal glands of fourteen primate species, Acta anatomica, 93:580-589.

  • Treasure T (1978). The ducts of Brunner’s glands. Journal of Anatomy, 127: 299-304.

  • Mohammadpour, A. A. (2011). Morphological and histochemical study of guinea pig duodenal submucosal glands, Bulgarian Journal of Veterinary Medicine, 14(4): 201−208.

  • Schumacher, U.; Duker, M.; Katoh, M.; Jorns, J.and Krause, W.J. (2004). Histochemical similarities of mucins produced by Brunner’s glands and pyloric glands : A comparative study. J Anat Rec Part A; 278(A): 540-550.

  • Bohe, H.; Bohe, M.; Lindstrom, C. and Ohlsson, K. (1995). Pancreatic secretory trypsin inhibitor in human Brunner’s glands. J.Gastroenterol., 30(1):90-95.

  • Kirkegaard, P.; Olsen, P. S.; Nexo, E.; Holst, J. J. and Poulsen, S. S. (1984). Effect of vasoactive intestinal polypeptide and somatostatin on secretion of epidermal growth factor and bicarbonate from Brunner’s glands. Gut., 25(11):1225-1229.

  • Obuoforibo, A. A. (1975). Mucosubstances in Brunner's glands of the mouse J. Anat., 119 ( 2): 287-294.


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  • A Comparative Histomorphological and Histochemical Study of the Goblet Cells and Brunner's Glands in the Duodenum of Rabbits and Rats

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Authors

Israa Jawad
Department of Physiology, Biochemical and Pharmacology/College of Veterinary Medicine/AL-Muthanna University, Iraq
Khalid Hadi Kadhim
Department of Physiology, Biochemical and Pharmacology/College of Veterinary Medicine/AL-Muthanna University, Iraq
Diyar M. H. Kadhim
Department of Anat. and Hist./College of Veterinary Medicine/AL-Muthanna University, Iraq
Dina Hamid Sadiq
Dep. of Anatomy/College of Nursing/University of Al-Basra, Iraq

Abstract


The purpose of this study was to describe, comparison the histological structures, histochemical features and distribution of the goblet cells and Brunner's glands in the duodenum of the rabbits and rats. The study was carried out on Ten samples of duodenum of each of rabbits and rats, immediately after slaughtering. The specimens were divided into cranial, descending and ascending parts. Five specimens was taken from each part of duodenum of each of the rabbits and rats and fixed in 10% formalin for 24 hours and treating by routine histological technique. Sections were stained by Hematoxylin and Eosin, Picric acid stain and Alcian blue PH 2.5 stains. Mean length of crypts of Lieberkuhn and number of each crypts, goblet cells and alveoli of Brunner's glands in duodenum of rabbits were more than that in the duodenum of rats. The mean length and number of crypts of Lieberkuhn was increase toward the last parts of the duodenum in each of the rabbits and rats. Goblet cells were globular cells between the columnar cells in the epithelium lined each of the villi and crypts of Lieberkuhn in the tunica mucosa of the duodenum in rabbits and rats and take positive reaction each of PAS and Alcian blue pH 2.5 stains. The mean number of goblet cells in crypts of Lieberkuhn in duodenum each rabbits and rats were increased toward the jejunum. Brunner’s glands were branched tubuloalveolar glands in the submucosa of each duodenal parts in rabbits, while absent in ascending part of rats duodenum, and these glands in rabbits duodenum were composed of two types of the cells (serous and mucous), while in rats duodenum only mucous cells, and stained with PAS stain only in duodenum of each of the rabbits and rats. The mean number of the alveoli of the Brunner's glands in the cranial part were more than that in other parts and decreased toward ascending part in rabbits duodenum, while absent in ascending part of rats duodenum.

Keywords


Slaughtering, Lieberkuhn, Tubuloalveolar.

References