Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Influence of Organic acids (Acetic, Citric acid and Blend) In vitro on growth of E. coli O157: H7: in Poultry feed


Affiliations
1 University of Maysan, Basic College of Education, Maysan, Iraq
2 Public Health Department, University of Baghdad, Baghdad, Iraq
     

   Subscribe/Renew Journal


The study carried out at the possibility of employ organic acids with chicken feed to inhibits microorganism porn in feed, that which causes deteriorate and corruption of this feed, as well as the transmission of diseases, which are a threat to public health. Ten samples were prepared from 0.25 g of poultry feed and sterilized with autoclave and then infected with E. coli O157:H7 bacteria then incubated for two hours. Organic acids were added to the poultry feed in different percentage of acetic acid, citric acid and blind (0.5, 1, and 1.5) and incubated for 24 hours. Bacterial count was performed for each sample through 60 min. The influence of the acid concentration was noted when compared between the different groups, the blend acids B3 (1.5%) groups (3.88±0.041) showed high significant effect (P > 0.05) than acetic and citric acid group (A, C) groups at 60 minutes of time. The available growth time of E. coli O157H7 that contaminated chicken feed acetic, citric acid and both (blend) of them (0.5, 1, 1.5) % on the growth of E. coli were showed exhibited tolerance for 120 minutes except the blend group (B3) 1.5%, was showed no growth (0.00) in all group after 180 minutes of incubation.

Keywords

Organic Acid, E. coli O157:H7, Blend, Feed.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Abdaljaleel, R.A., Al-Ajeeli, M., Jameel, Y., Hashim, M.M., Bailey, C.A. (2018). Assessing effects of yeast cell wall supplementation on threonine requirements in broilers as measured by performance and intestinal morphology. 2018 Poultry Science 97:2473–2478 http://dx.doi.org/10.3382/ps/pey095.

  • Ali, Nihad Abdul-Lateef, Salah M.H. AL-Sherify, Alaa K. Alshojiry, Yasser Jameel. (2018). Effect of Adding Different Concentrations of the Mint oil Mentha spicata L. to the Ration on Some Blood Traits of Broiler Chicks Ross 308. Journal of Global Pharmacy Technology. 2018; 10(01):60-64.

  • Al-Kassie, G. A. M., Al-Jumaa, Y. M. F., & Jameel, Y. J. (2008a). Effect of probiotic (Aspergillus niger) and prebiotic (Taraxacum officinale) on blood picture and biochemical properties of broiler chicks. International Journal of Poultry Science, 7(12), 1182-1184.

  • Al-Kassie, G. A. M., Mohammed, M. F., Hamood, M. F., & Jameel, Y. J. (2008b). The effect of anise and rosemary on the microbial balance in gastro intestinal tract for broiler chicks. International Journal of Poultry Science, 7(6), 610-612.

  • Baronofsky, J. J., Schreurs, W. J., & Kashket, E. R. (1984). Uncoupling by acetic acid limits growth of and acetogenesis by Clostridium thermoaceticum. Applied and environmental microbiology, 48(6), 1134-1139.

  • Bennett, H. S., & Luft, J. H. (1959). s-Collidine as a basis for buffering fixatives. The Journal of biophysical and biochemical cytology, 6(1), 113.

  • Biggs, P., & Parsons, C. M. (2007). The effects of several oligosaccharides on true amino acid digestibility and true metabolizable energy in cecectomized and conventional roosters. Poultry science, 86(6), 1161-1165.

  • D Mello, J. P. F. (2004). Microbiology of animal feeds. FAO ANIMAL PRODUCTION AND HEALTH PAPER, 89-106.

  • Diaz-Sanchez, S., D'Souza, D., Biswas, D., & Hanning, I. (2015). Botanical alternatives to antibiotics for use in organic poultry production. Poultry science, 94(6), 1419-1430.

  • Dibner, J. J., & Buttin, P. (2002). Use of organic acids as a model to study the impact of gut microflora on nutrition and metabolism. Journal of Applied Poultry Research, 11(4), 453-463.

  • D'mello, J. P. F. (2002). Microbiology of animal feeds. Formerly of the scottish agricultural college (sac), west mains road, edinburgh eh9 3jg, united kingdom.

  • Humphrey, T. J., & Lanning, D. G. (1988). The vertical transmission of salmonellas and formic acid treatment of chicken feed: a possible strategy for control. Epidemiology & Infection, 100(1), 43-49.

  • Hunter PA, Dawson S, French GL, et al. Antimicrobial-resistant pathogens in animals and man: prescribing, practices and policies. J Antimicrob Chemother. 2010;65(Suppl 1):3‒17.

  • Islam, M. Z., Khandaker, Z. H., Chowdhury, S. D., & Islam, K. M. S. (2008). Effect of citric acid and acetic acid on the performance of broilers. J. Bangladesh Agri. Univ, 2, 315-320.

  • Jameel, Y. J. (2017). Evaluation of probiotic, prebiotic and synbiotic on starter broilers performance subjected to bursa vaccine and clostridium perfringens challenge. IJABR, VOL.7 (2) 2017: 373-377.

  • Jameel, Y.J. (2018). Effect of prebiotic plus xylanase enzymes in reduced-energy high wheat Midd diets on broilers subjected to bursa vaccine and Clostridium perfringens challenge under heat stress. Journal of Global Pharmacy Technology. 2018; 10(01):53-59.

  • McFarland, J. (1907). The nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. Journal of the American Medical Association, 49(14), 1176-1178.

  • Nursey, I. 1997. Control of Salmonella. Journal of Veterinary Medicine, 10: 415-22.

  • Theron, M. M., & Lues, J. R. (2010). Organic acids and food preservation. CRC Press.

  • Warth, A. D. (1991). Mechanism of action of benzoic acid on Zygosaccharomyces bailii: effects on glycolytic metabolite levels, energy production, and intracellular pH. Applied and environmental microbiology, 57(12), 3410-3414.

  • Waseem Mirza, M., Rehman, Z. U., & Mukhtar, N. (2016). Use of organic acids as potential feed additives in poultry production. Journal of World's Poultry Research, 6(3), 105-116.

  • Wendel, A. M., Johnson, D. H., Sharapov, U., Grant, J., Archer, J. R., Monson, T., ... & Davis, J. P. (2009). Multistate outbreak of Escherichia coli O157: H7 infection associated with consumption of packaged spinach, August–September 2006: the Wisconsin investigation. Clinical infectious diseases, 48(8), 1079-1086.

  • Young, K. M., & Foegeding, P. M. (1993). Acetic, lactic and citric acids and pH inhibition of Listeria monocytogenes Scott A and the effect on intracellular pH. The Journal of applied bacteriology, 74(5), 515-520.


Abstract Views: 231

PDF Views: 0




  • Influence of Organic acids (Acetic, Citric acid and Blend) In vitro on growth of E. coli O157: H7: in Poultry feed

Abstract Views: 231  |  PDF Views: 0

Authors

Taha H. Al-Yasiri
University of Maysan, Basic College of Education, Maysan, Iraq
Meyada F. Mohammed
Public Health Department, University of Baghdad, Baghdad, Iraq

Abstract


The study carried out at the possibility of employ organic acids with chicken feed to inhibits microorganism porn in feed, that which causes deteriorate and corruption of this feed, as well as the transmission of diseases, which are a threat to public health. Ten samples were prepared from 0.25 g of poultry feed and sterilized with autoclave and then infected with E. coli O157:H7 bacteria then incubated for two hours. Organic acids were added to the poultry feed in different percentage of acetic acid, citric acid and blind (0.5, 1, and 1.5) and incubated for 24 hours. Bacterial count was performed for each sample through 60 min. The influence of the acid concentration was noted when compared between the different groups, the blend acids B3 (1.5%) groups (3.88±0.041) showed high significant effect (P > 0.05) than acetic and citric acid group (A, C) groups at 60 minutes of time. The available growth time of E. coli O157H7 that contaminated chicken feed acetic, citric acid and both (blend) of them (0.5, 1, 1.5) % on the growth of E. coli were showed exhibited tolerance for 120 minutes except the blend group (B3) 1.5%, was showed no growth (0.00) in all group after 180 minutes of incubation.

Keywords


Organic Acid, E. coli O157:H7, Blend, Feed.

References