Research Journal of Pharmacy and Technology

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Development of Validated Q-absorbance Ratio, Derivative Spectrophotometric and Stability Indicating RP-HPLC Methods for the Simultaneous estimation of Losartan Potassium & Hydrochlorothiazide in Pharmaceutical Dosage Form and Comparison of the Results by ANOVA


Affiliations
1 Maharajah’s College of Pharmacy, Phool Baugh, Vizianagaram, Andhra Pradesh, India
     

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Hypertension is estimated to cause 7.5 million deaths, leading to 12.8 % of the total of all deaths worldwide. In the recent years, the combination of Losartan Potassium and Hydrochlorothiazide has attained significant popularity in reducing the risk of heart diseases particularly in the patients with hypertension and ventricular hypertrophy. The literature survey revealed that there were several chromatographic and spectroscopic methods for the simultaneous estimation of the selected drugs but it was noted that there were no reported comparative studies of the developed methods. Hence the authors attempted to develop new validated Q- absorbance ratio, Derivative spectrophotometric and Stability indicating RP-HPLC methods for the simultaneous estimation of Losartan Potassium and Hydrochlorothiazide in pharmaceutical dosage form. The authors also propose to compare the results obtained by ANOVA. The two Spectrophotometric methods were carried out using an Agilent Cary 60 UV-Visible double beam Spectrophotometer. In Q-absorbance ratio method, samples were measured at two wavelengths, 230 nm (isoabsorptive point) and 225.5 nm (λmax of Hydrochlorothiazide). In Derivative spectroscopy, analysis was performed by derivatizing the zero order spectrum to the corresponding second order spectrum. Stability indicating RP-HPLC method was performed using Eclipse XDB C18 column where the samples were analyzed using a mixture of Acetonitrile and Water in the ratio of 70:30 % v/v as mobile phase at a flow rate of 1 ml/min. The drugs were monitored at 230 nm. The retention times for Losartan Potassium and Hydrochlorothiazide were found to be 1.010 minutes and 1.387 minutes respectively. The proposed methods were validated as per ICH guidelines. The results obtained from the three developed methods were then compared by ANOVA which indicated that the proposed methods can successfully be used for the analysis of Losartan Potassium and Hydrochlorothiazide in bulk or in combined dosage forms.

Keywords

Losartan Potassium, Hydrochlorothiazide, Q-Absorbance Ratio Method, Derivative Spectrophotometric Method, Stability Indicating RP-HPLC, ANOVA.
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  • Development of Validated Q-absorbance Ratio, Derivative Spectrophotometric and Stability Indicating RP-HPLC Methods for the Simultaneous estimation of Losartan Potassium & Hydrochlorothiazide in Pharmaceutical Dosage Form and Comparison of the Results by ANOVA

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Authors

V. V. S. Ashritha
Maharajah’s College of Pharmacy, Phool Baugh, Vizianagaram, Andhra Pradesh, India
N. Usharani
Maharajah’s College of Pharmacy, Phool Baugh, Vizianagaram, Andhra Pradesh, India
Soudamini Alekhacharan
Maharajah’s College of Pharmacy, Phool Baugh, Vizianagaram, Andhra Pradesh, India
B. Alekhya
Maharajah’s College of Pharmacy, Phool Baugh, Vizianagaram, Andhra Pradesh, India
R. Venkatesh
Maharajah’s College of Pharmacy, Phool Baugh, Vizianagaram, Andhra Pradesh, India

Abstract


Hypertension is estimated to cause 7.5 million deaths, leading to 12.8 % of the total of all deaths worldwide. In the recent years, the combination of Losartan Potassium and Hydrochlorothiazide has attained significant popularity in reducing the risk of heart diseases particularly in the patients with hypertension and ventricular hypertrophy. The literature survey revealed that there were several chromatographic and spectroscopic methods for the simultaneous estimation of the selected drugs but it was noted that there were no reported comparative studies of the developed methods. Hence the authors attempted to develop new validated Q- absorbance ratio, Derivative spectrophotometric and Stability indicating RP-HPLC methods for the simultaneous estimation of Losartan Potassium and Hydrochlorothiazide in pharmaceutical dosage form. The authors also propose to compare the results obtained by ANOVA. The two Spectrophotometric methods were carried out using an Agilent Cary 60 UV-Visible double beam Spectrophotometer. In Q-absorbance ratio method, samples were measured at two wavelengths, 230 nm (isoabsorptive point) and 225.5 nm (λmax of Hydrochlorothiazide). In Derivative spectroscopy, analysis was performed by derivatizing the zero order spectrum to the corresponding second order spectrum. Stability indicating RP-HPLC method was performed using Eclipse XDB C18 column where the samples were analyzed using a mixture of Acetonitrile and Water in the ratio of 70:30 % v/v as mobile phase at a flow rate of 1 ml/min. The drugs were monitored at 230 nm. The retention times for Losartan Potassium and Hydrochlorothiazide were found to be 1.010 minutes and 1.387 minutes respectively. The proposed methods were validated as per ICH guidelines. The results obtained from the three developed methods were then compared by ANOVA which indicated that the proposed methods can successfully be used for the analysis of Losartan Potassium and Hydrochlorothiazide in bulk or in combined dosage forms.

Keywords


Losartan Potassium, Hydrochlorothiazide, Q-Absorbance Ratio Method, Derivative Spectrophotometric Method, Stability Indicating RP-HPLC, ANOVA.

References